Potential antidiabetic and antioxidant activities of a heliangolide sesquiterpene lactone isolated from Helianthus annuus L. leaves
Heliangolide is a naturally occurring sesquiterpene lactone and its derivatives are biologically active compounds present in most medicinal plants. This study evaluated the antioxidant and antidiabetic properties of a heliangolide sesquiterpene lactone isolated from Helianthus annuus L. leaves. The...
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2020-06-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2020-0019 |
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| author | Onoja Samuel O. Nnadi Charles O. Udem Samuel C. Anaga Aruh O. |
| author_facet | Onoja Samuel O. Nnadi Charles O. Udem Samuel C. Anaga Aruh O. |
| author_sort | Onoja Samuel O. |
| collection | DOAJ |
| description | Heliangolide is a naturally occurring sesquiterpene lactone and its derivatives are biologically active compounds present in most medicinal plants. This study evaluated the antioxidant and antidiabetic properties of a heliangolide sesquiterpene lactone isolated from Helianthus annuus L. leaves. The heliangolide sesquiterpene lactone was isolated through a combination of solvent-solvent partitioning, column chromatography, thin layer chromatography and high-performance liquid chromatography techniques. The antioxidant activity of the compound was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide radical scavenging assays while the antidiabetic effects were investigated in alloxan-induced diabetic rats. The heliangolide derivative at the concentration of 954.2 µmol L−1 showed 23.7 % DPPH and 26 % nitric oxide radical inhibitions compared with 96.6 and 50.9 %, resp., displayed by the controls (2,271.2 µmol L−1). It also reduced the fasting blood glucose (FBG) levels in a time-dependent manner. The highest activity was recorded within 6 h post-treatment at 0.2 mmol kg−1 bm. The heliangolide derivative exhibited significant (p < 0.05) antioxidant and antidiabetic properties and provides a basis for further development of constituents of Helianthus annuus leaves for the management of such diseases. |
| format | Article |
| id | doaj-art-457289df60d74e83a95aab0515c21fd5 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2020-06-01 |
| publisher | Sciendo |
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| series | Acta Pharmaceutica |
| spelling | doaj-art-457289df60d74e83a95aab0515c21fd52025-02-02T22:59:43ZengSciendoActa Pharmaceutica1846-95582020-06-0170221522610.2478/acph-2020-0019acph-2020-0019Potential antidiabetic and antioxidant activities of a heliangolide sesquiterpene lactone isolated from Helianthus annuus L. leavesOnoja Samuel O.0Nnadi Charles O.1Udem Samuel C.2Anaga Aruh O.3Department of Veterinary Physiology and Pharmacology, Michael Okpara University of Agriculture Umudike, PMB 7267 Umuahia, NigeriaDepartment of Pharmaceutical and Medicinal Chemistry, University of Nigeria, Nsukka, 410001 Enugu State, NigeriaDepartment of Veterinary Physiology and Pharmacology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka 410001 Enugu State, NigeriaDepartment of Veterinary Physiology and Pharmacology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka 410001 Enugu State, NigeriaHeliangolide is a naturally occurring sesquiterpene lactone and its derivatives are biologically active compounds present in most medicinal plants. This study evaluated the antioxidant and antidiabetic properties of a heliangolide sesquiterpene lactone isolated from Helianthus annuus L. leaves. The heliangolide sesquiterpene lactone was isolated through a combination of solvent-solvent partitioning, column chromatography, thin layer chromatography and high-performance liquid chromatography techniques. The antioxidant activity of the compound was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide radical scavenging assays while the antidiabetic effects were investigated in alloxan-induced diabetic rats. The heliangolide derivative at the concentration of 954.2 µmol L−1 showed 23.7 % DPPH and 26 % nitric oxide radical inhibitions compared with 96.6 and 50.9 %, resp., displayed by the controls (2,271.2 µmol L−1). It also reduced the fasting blood glucose (FBG) levels in a time-dependent manner. The highest activity was recorded within 6 h post-treatment at 0.2 mmol kg−1 bm. The heliangolide derivative exhibited significant (p < 0.05) antioxidant and antidiabetic properties and provides a basis for further development of constituents of Helianthus annuus leaves for the management of such diseases.https://doi.org/10.2478/acph-2020-0019helianthus annuusheliangolide derivativeantidiabeticantioxidant |
| spellingShingle | Onoja Samuel O. Nnadi Charles O. Udem Samuel C. Anaga Aruh O. Potential antidiabetic and antioxidant activities of a heliangolide sesquiterpene lactone isolated from Helianthus annuus L. leaves Acta Pharmaceutica helianthus annuus heliangolide derivative antidiabetic antioxidant |
| title | Potential antidiabetic and antioxidant activities of a heliangolide sesquiterpene lactone isolated from Helianthus annuus L. leaves |
| title_full | Potential antidiabetic and antioxidant activities of a heliangolide sesquiterpene lactone isolated from Helianthus annuus L. leaves |
| title_fullStr | Potential antidiabetic and antioxidant activities of a heliangolide sesquiterpene lactone isolated from Helianthus annuus L. leaves |
| title_full_unstemmed | Potential antidiabetic and antioxidant activities of a heliangolide sesquiterpene lactone isolated from Helianthus annuus L. leaves |
| title_short | Potential antidiabetic and antioxidant activities of a heliangolide sesquiterpene lactone isolated from Helianthus annuus L. leaves |
| title_sort | potential antidiabetic and antioxidant activities of a heliangolide sesquiterpene lactone isolated from helianthus annuus l leaves |
| topic | helianthus annuus heliangolide derivative antidiabetic antioxidant |
| url | https://doi.org/10.2478/acph-2020-0019 |
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