Virulence and Antimicrobial Resistance Patterns of Salmonella spp. Recovered From Migratory and Captive Wild Birds
ABSTRACT Background Salmonella spp., especially those are resistant to extended‐spectrum β‐lactamase (ESBL), are considered as major concern to global health due to their emergence and dissemination. Aim The aim of this study was to investigate the virulence and antimicrobial resistance (AMR) profil...
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| Format: | Article |
| Language: | English |
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Wiley
2024-11-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.70102 |
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| author | Ruhena Begum Nilima Akther Asha Diponkar Chandra Chanda Dipu Milton Roy Asikur Rahman Md. Shahidur Rahman Chowdhury Hemayet Hossain Md. Rafiqul Islam Md Bashir Uddin Md. Mahfujur Rahman Md. Mukter Hossain |
| author_facet | Ruhena Begum Nilima Akther Asha Diponkar Chandra Chanda Dipu Milton Roy Asikur Rahman Md. Shahidur Rahman Chowdhury Hemayet Hossain Md. Rafiqul Islam Md Bashir Uddin Md. Mahfujur Rahman Md. Mukter Hossain |
| author_sort | Ruhena Begum |
| collection | DOAJ |
| description | ABSTRACT Background Salmonella spp., especially those are resistant to extended‐spectrum β‐lactamase (ESBL), are considered as major concern to global health due to their emergence and dissemination. Aim The aim of this study was to investigate the virulence and antimicrobial resistance (AMR) profile of Salmonella spp. from migratory and captive wild birds. Method A total 262 faecal samples were collected, and the identification of Salmonella spp. was carried out using a standard culture and PCR as well as molecular detection of virulence and AMR genes. Results The overall prevalence of Salmonella was determined to be 30.92% (95% CI = 25.63–36.75). Migratory birds exhibited highest prevalence (38.10%), whereas wild birds in captivity showed a lower prevalence (23.40%). The agfA gene was detected at a higher rate at 24.69%. Salmonella spp. exhibited 100% resistance to tetracycline, followed by 58% ampicillin and 46% streptomycin. In addition, there was a resistance rate to ceftriaxone of 17% and to colistin sulphate of 25%. Interestingly, levofloxacin alone displayed 100% sensitivity across all isolates, while ciprofloxacin and azithromycin showed 73% and 64% sensitivity, respectively. The MAR index was 0.25 and 0.42, and 74.07% of all isolates showed multidrug resistance (MDR). It was shown that migratory and captive wild birds contained ESBL genes blaTEM (94.34% and 49.06%) and blaSHV (13.33% and 10%), respectively. Genes responsible for sulphonamide (sul1) resistance were detected in 13.33% and 79% of wild and migratory birds, respectively. Conclusion Salmonella has been found in captive wild and migratory birds and could act as reservoirs for the transmission of MDR and ESBL bacteria. |
| format | Article |
| id | doaj-art-45722f4ce92f48e7ab57c28c46b87a20 |
| institution | Kabale University |
| issn | 2053-1095 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
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| series | Veterinary Medicine and Science |
| spelling | doaj-art-45722f4ce92f48e7ab57c28c46b87a202025-01-20T17:16:45ZengWileyVeterinary Medicine and Science2053-10952024-11-01106n/an/a10.1002/vms3.70102Virulence and Antimicrobial Resistance Patterns of Salmonella spp. Recovered From Migratory and Captive Wild BirdsRuhena Begum0Nilima Akther Asha1Diponkar Chandra Chanda Dipu2Milton Roy3Asikur Rahman4Md. Shahidur Rahman Chowdhury5Hemayet Hossain6Md. Rafiqul Islam7Md Bashir Uddin8Md. Mahfujur Rahman9Md. Mukter Hossain10Department of Medicine Sylhet Agricultural University Sylhet BangladeshDepartment of Medicine Sylhet Agricultural University Sylhet BangladeshDepartment of Medicine Sylhet Agricultural University Sylhet BangladeshDepartment of Medicine Sylhet Agricultural University Sylhet BangladeshDepartment of Medicine Sylhet Agricultural University Sylhet BangladeshDepartment of Medicine Sylhet Agricultural University Sylhet BangladeshDepartment of Anatomy and Histology Sylhet Agricultural University Sylhet BangladeshDepartment of Medicine Sylhet Agricultural University Sylhet BangladeshDepartment of Medicine Sylhet Agricultural University Sylhet BangladeshDepartment of Medicine Sylhet Agricultural University Sylhet BangladeshDepartment of Medicine Sylhet Agricultural University Sylhet BangladeshABSTRACT Background Salmonella spp., especially those are resistant to extended‐spectrum β‐lactamase (ESBL), are considered as major concern to global health due to their emergence and dissemination. Aim The aim of this study was to investigate the virulence and antimicrobial resistance (AMR) profile of Salmonella spp. from migratory and captive wild birds. Method A total 262 faecal samples were collected, and the identification of Salmonella spp. was carried out using a standard culture and PCR as well as molecular detection of virulence and AMR genes. Results The overall prevalence of Salmonella was determined to be 30.92% (95% CI = 25.63–36.75). Migratory birds exhibited highest prevalence (38.10%), whereas wild birds in captivity showed a lower prevalence (23.40%). The agfA gene was detected at a higher rate at 24.69%. Salmonella spp. exhibited 100% resistance to tetracycline, followed by 58% ampicillin and 46% streptomycin. In addition, there was a resistance rate to ceftriaxone of 17% and to colistin sulphate of 25%. Interestingly, levofloxacin alone displayed 100% sensitivity across all isolates, while ciprofloxacin and azithromycin showed 73% and 64% sensitivity, respectively. The MAR index was 0.25 and 0.42, and 74.07% of all isolates showed multidrug resistance (MDR). It was shown that migratory and captive wild birds contained ESBL genes blaTEM (94.34% and 49.06%) and blaSHV (13.33% and 10%), respectively. Genes responsible for sulphonamide (sul1) resistance were detected in 13.33% and 79% of wild and migratory birds, respectively. Conclusion Salmonella has been found in captive wild and migratory birds and could act as reservoirs for the transmission of MDR and ESBL bacteria.https://doi.org/10.1002/vms3.70102antimicrobial resistancemigratory birdsresistance geneSalmonellavirulent genewild birds |
| spellingShingle | Ruhena Begum Nilima Akther Asha Diponkar Chandra Chanda Dipu Milton Roy Asikur Rahman Md. Shahidur Rahman Chowdhury Hemayet Hossain Md. Rafiqul Islam Md Bashir Uddin Md. Mahfujur Rahman Md. Mukter Hossain Virulence and Antimicrobial Resistance Patterns of Salmonella spp. Recovered From Migratory and Captive Wild Birds Veterinary Medicine and Science antimicrobial resistance migratory birds resistance gene Salmonella virulent gene wild birds |
| title | Virulence and Antimicrobial Resistance Patterns of Salmonella spp. Recovered From Migratory and Captive Wild Birds |
| title_full | Virulence and Antimicrobial Resistance Patterns of Salmonella spp. Recovered From Migratory and Captive Wild Birds |
| title_fullStr | Virulence and Antimicrobial Resistance Patterns of Salmonella spp. Recovered From Migratory and Captive Wild Birds |
| title_full_unstemmed | Virulence and Antimicrobial Resistance Patterns of Salmonella spp. Recovered From Migratory and Captive Wild Birds |
| title_short | Virulence and Antimicrobial Resistance Patterns of Salmonella spp. Recovered From Migratory and Captive Wild Birds |
| title_sort | virulence and antimicrobial resistance patterns of salmonella spp recovered from migratory and captive wild birds |
| topic | antimicrobial resistance migratory birds resistance gene Salmonella virulent gene wild birds |
| url | https://doi.org/10.1002/vms3.70102 |
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