Masculinization of betta fish Betta splendens by embryo immersion in extract of purwoceng Pimpinella alpina

ABSTRACT   This study aimed to examine the effect of extract of purwoceng Pimpinella alpina for masculinization of Betta splendens. This research used completely randomized design with three treatments that were distinguished by doses of purwoceng extract, which were 10, 20, and 30 µL/L, and 0 µL/L...

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Main Authors: Harton Arfah, Dinar Tri Soelistyowati, Asep Bulkini
Format: Article
Language:English
Published: Asosiasi Sains Akuakultur Indonesia 2015-05-01
Series:Jurnal Akuakultur Indonesia
Online Access:https://journal.ipb.ac.id/index.php/jai/article/view/9369
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author Harton Arfah
Dinar Tri Soelistyowati
Asep Bulkini
author_facet Harton Arfah
Dinar Tri Soelistyowati
Asep Bulkini
author_sort Harton Arfah
collection DOAJ
description ABSTRACT   This study aimed to examine the effect of extract of purwoceng Pimpinella alpina for masculinization of Betta splendens. This research used completely randomized design with three treatments that were distinguished by doses of purwoceng extract, which were 10, 20, and 30 µL/L, and 0 µL/L as control. The treatments were given by immersion to 35 embryos at eye spots phase or about 28-hours after fertilization, for eight hours. The immersion process was done for 8 hours. The result showed that 20 µL/L dose of purwoceng extract produced 62.66% male, and hatching rate was 85.71%. This male percentage was higher compared to control (45.91%), but the hatching rate was lower than that of control (98.57%). At the higher dose (30 µL/L), male fish population and hatching rate reduced respectively 39.72% and 68.57%, respectively.   Keywords: masculinization, embryo immersion, purwoceng extract, Betta splendens     ABSTRAK   Penelitian ini bertujuan untuk mengevaluasi pengaruh dosis ekstrak purwoceng Pimpinella alpina extract dalam maskulinisasi ikan cupang hias Betta splendens. Penelitian ini menggunakan metode rancangan acak lengkap dengan tiga perlakuan dan tiga ulangan dosis ekstrak purwoceng. yaitu 10, 20, dan 30 µL/L, serta perlakuan 0 µL/L sebagai kontrol. Perlakuan diberikan melalui perendaman selama delapan jam terhadap 35 embrio saat memasuki fase bintik mata atau sekitar jam ke-28 setelah pembuahan. Hasil penelitian menunjukkan bahwa dosis ekstrak purwoceng 20 µL/L menghasilkan persentase ikan jantan sebesar 62,66%, dan daya tetas telur sebesar 85,71%. Persentase jantan tersebut lebih tinggi daripada kontrol (45,1%), tetapi derajat penetasannya lebih rendah daripada kontrol (98,57%). Pada dosis yang lebih tinggi (30 µL/L), populasi ikan jantan (39,72%), dan derajat penetasan (68,57%) adalah menurun.   Kata kunci: maskulinisasi, perendaman embrio, ekstrak purwoceng, Betta splendens
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issn 1412-5269
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publisher Asosiasi Sains Akuakultur Indonesia
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spelling doaj-art-456a391f79874c85a760e949d206fd422025-01-24T17:59:18ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002015-05-0112210.19027/jai.12.144-1497833Masculinization of betta fish Betta splendens by embryo immersion in extract of purwoceng Pimpinella alpinaHarton Arfah0Dinar Tri Soelistyowati1Asep Bulkini2Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Dramaga Bogor 16680Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Dramaga Bogor 16680Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Dramaga Bogor 16680ABSTRACT   This study aimed to examine the effect of extract of purwoceng Pimpinella alpina for masculinization of Betta splendens. This research used completely randomized design with three treatments that were distinguished by doses of purwoceng extract, which were 10, 20, and 30 µL/L, and 0 µL/L as control. The treatments were given by immersion to 35 embryos at eye spots phase or about 28-hours after fertilization, for eight hours. The immersion process was done for 8 hours. The result showed that 20 µL/L dose of purwoceng extract produced 62.66% male, and hatching rate was 85.71%. This male percentage was higher compared to control (45.91%), but the hatching rate was lower than that of control (98.57%). At the higher dose (30 µL/L), male fish population and hatching rate reduced respectively 39.72% and 68.57%, respectively.   Keywords: masculinization, embryo immersion, purwoceng extract, Betta splendens     ABSTRAK   Penelitian ini bertujuan untuk mengevaluasi pengaruh dosis ekstrak purwoceng Pimpinella alpina extract dalam maskulinisasi ikan cupang hias Betta splendens. Penelitian ini menggunakan metode rancangan acak lengkap dengan tiga perlakuan dan tiga ulangan dosis ekstrak purwoceng. yaitu 10, 20, dan 30 µL/L, serta perlakuan 0 µL/L sebagai kontrol. Perlakuan diberikan melalui perendaman selama delapan jam terhadap 35 embrio saat memasuki fase bintik mata atau sekitar jam ke-28 setelah pembuahan. Hasil penelitian menunjukkan bahwa dosis ekstrak purwoceng 20 µL/L menghasilkan persentase ikan jantan sebesar 62,66%, dan daya tetas telur sebesar 85,71%. Persentase jantan tersebut lebih tinggi daripada kontrol (45,1%), tetapi derajat penetasannya lebih rendah daripada kontrol (98,57%). Pada dosis yang lebih tinggi (30 µL/L), populasi ikan jantan (39,72%), dan derajat penetasan (68,57%) adalah menurun.   Kata kunci: maskulinisasi, perendaman embrio, ekstrak purwoceng, Betta splendens https://journal.ipb.ac.id/index.php/jai/article/view/9369
spellingShingle Harton Arfah
Dinar Tri Soelistyowati
Asep Bulkini
Masculinization of betta fish Betta splendens by embryo immersion in extract of purwoceng Pimpinella alpina
Jurnal Akuakultur Indonesia
title Masculinization of betta fish Betta splendens by embryo immersion in extract of purwoceng Pimpinella alpina
title_full Masculinization of betta fish Betta splendens by embryo immersion in extract of purwoceng Pimpinella alpina
title_fullStr Masculinization of betta fish Betta splendens by embryo immersion in extract of purwoceng Pimpinella alpina
title_full_unstemmed Masculinization of betta fish Betta splendens by embryo immersion in extract of purwoceng Pimpinella alpina
title_short Masculinization of betta fish Betta splendens by embryo immersion in extract of purwoceng Pimpinella alpina
title_sort masculinization of betta fish betta splendens by embryo immersion in extract of purwoceng pimpinella alpina
url https://journal.ipb.ac.id/index.php/jai/article/view/9369
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AT dinartrisoelistyowati masculinizationofbettafishbettasplendensbyembryoimmersioninextractofpurwocengpimpinellaalpina
AT asepbulkini masculinizationofbettafishbettasplendensbyembryoimmersioninextractofpurwocengpimpinellaalpina