Dissection of the genetic basis and molecular mechanism of ovule number per ovary in oilseed rape (Brassica napus L.)
Ovule number per ovary (ONPO) determines the maximum potential of seed number per fruit that is a direct component of seed yield in crops. This study aimed to dissect the genetic basis and molecular mechanism of ONPO using a newly developed doubled haploid (DH) population in oilseed rape. In all the...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1489490/full |
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| author | Muslim Qadir Muslim Qadir Muslim Qadir Xinyi Lin Farhan Nabi Kishore Kumar Ashok Xue-Rong Zhou Qingbin Sun Peiman Shi Xinfa Wang Jiaqin Shi Hanzhong Wang |
| author_facet | Muslim Qadir Muslim Qadir Muslim Qadir Xinyi Lin Farhan Nabi Kishore Kumar Ashok Xue-Rong Zhou Qingbin Sun Peiman Shi Xinfa Wang Jiaqin Shi Hanzhong Wang |
| author_sort | Muslim Qadir |
| collection | DOAJ |
| description | Ovule number per ovary (ONPO) determines the maximum potential of seed number per fruit that is a direct component of seed yield in crops. This study aimed to dissect the genetic basis and molecular mechanism of ONPO using a newly developed doubled haploid (DH) population in oilseed rape. In all the four investigated environments, the ONPO of 201 DH lines exhibited normal distribution with a wide variation from 22.6 to 41.8, suggesting quantitative inheritance appropriate for mapping QTL. A skeleton genetic map of 2111 markers within 19 linkage groups was developed, with a total of 1715.71 cM in length and an average of 0.82 cM between markers. Linkage mapping identified ten QTLs that were distributed on eight chromosomes and explained 7.0-15.9% of the phenotypic variance. Among these, four were identical to the reported and two were repeatedly detected with relatively large effects, highlighting their potential for marker-assisted selection. Phytohormone quantification of ovaries (at the ovule initiation stage) from two pools of high and low ONPO lines showed significant differences in the levels of nine sub-types of phytohormones, suggesting their important roles in regulating ovule number. Transcriptomic analysis identified 7689 differentially expressed genes (DEGs) between the two pools, of which nearly half were enriched into functional categories of reported genes regulating ONPO, including protein, RNA, signalling, miscellaneous, development, hormone metabolism, and tetrapyrrole synthesis. Integration of linkage QTL mapping, transcriptome sequencing and BLAST analysis identified 15 homologues of reported ovule number genes and 327 DEGs in the QTL regions, which were considered as direct and potential candidate genes. These findings propose further insights into the genetic basis and molecular mechanisms of ONPO, which will facilitate future gene cloning and genetic improvement for enhancing seed yield in oilseed rape. |
| format | Article |
| id | doaj-art-4569f1a1352441c08ef0fb2ef613dea4 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-4569f1a1352441c08ef0fb2ef613dea42025-01-28T06:41:30ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.14894901489490Dissection of the genetic basis and molecular mechanism of ovule number per ovary in oilseed rape (Brassica napus L.)Muslim Qadir0Muslim Qadir1Muslim Qadir2Xinyi Lin3Farhan Nabi4Kishore Kumar Ashok5Xue-Rong Zhou6Qingbin Sun7Peiman Shi8Xinfa Wang9Jiaqin Shi10Hanzhong Wang11Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan, Hubei, ChinaCollege of Agriculture, South China Agricultural University, Guangzhou, ChinaIntegrative Agriculture Department, College of Agriculture and Veterinary Medicine, United Arab Emirates University (UAEU), Abu Dhabi, United Arab EmiratesOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan, Hubei, ChinaCollege of Agriculture, South China Agricultural University, Guangzhou, ChinaIntegrative Agriculture Department, College of Agriculture and Veterinary Medicine, United Arab Emirates University (UAEU), Abu Dhabi, United Arab EmiratesCommonwealth Scientific and Industrial Research Organization (CSIRO) Agriculture Food, Canberra, ACT, AustraliaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan, Hubei, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan, Hubei, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan, Hubei, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan, Hubei, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan, Hubei, ChinaOvule number per ovary (ONPO) determines the maximum potential of seed number per fruit that is a direct component of seed yield in crops. This study aimed to dissect the genetic basis and molecular mechanism of ONPO using a newly developed doubled haploid (DH) population in oilseed rape. In all the four investigated environments, the ONPO of 201 DH lines exhibited normal distribution with a wide variation from 22.6 to 41.8, suggesting quantitative inheritance appropriate for mapping QTL. A skeleton genetic map of 2111 markers within 19 linkage groups was developed, with a total of 1715.71 cM in length and an average of 0.82 cM between markers. Linkage mapping identified ten QTLs that were distributed on eight chromosomes and explained 7.0-15.9% of the phenotypic variance. Among these, four were identical to the reported and two were repeatedly detected with relatively large effects, highlighting their potential for marker-assisted selection. Phytohormone quantification of ovaries (at the ovule initiation stage) from two pools of high and low ONPO lines showed significant differences in the levels of nine sub-types of phytohormones, suggesting their important roles in regulating ovule number. Transcriptomic analysis identified 7689 differentially expressed genes (DEGs) between the two pools, of which nearly half were enriched into functional categories of reported genes regulating ONPO, including protein, RNA, signalling, miscellaneous, development, hormone metabolism, and tetrapyrrole synthesis. Integration of linkage QTL mapping, transcriptome sequencing and BLAST analysis identified 15 homologues of reported ovule number genes and 327 DEGs in the QTL regions, which were considered as direct and potential candidate genes. These findings propose further insights into the genetic basis and molecular mechanisms of ONPO, which will facilitate future gene cloning and genetic improvement for enhancing seed yield in oilseed rape.https://www.frontiersin.org/articles/10.3389/fpls.2024.1489490/fullovule number per ovaryQTL mappingdifferentially expressed genesphytohormonescandidate genesBrassica napus |
| spellingShingle | Muslim Qadir Muslim Qadir Muslim Qadir Xinyi Lin Farhan Nabi Kishore Kumar Ashok Xue-Rong Zhou Qingbin Sun Peiman Shi Xinfa Wang Jiaqin Shi Hanzhong Wang Dissection of the genetic basis and molecular mechanism of ovule number per ovary in oilseed rape (Brassica napus L.) Frontiers in Plant Science ovule number per ovary QTL mapping differentially expressed genes phytohormones candidate genes Brassica napus |
| title | Dissection of the genetic basis and molecular mechanism of ovule number per ovary in oilseed rape (Brassica napus L.) |
| title_full | Dissection of the genetic basis and molecular mechanism of ovule number per ovary in oilseed rape (Brassica napus L.) |
| title_fullStr | Dissection of the genetic basis and molecular mechanism of ovule number per ovary in oilseed rape (Brassica napus L.) |
| title_full_unstemmed | Dissection of the genetic basis and molecular mechanism of ovule number per ovary in oilseed rape (Brassica napus L.) |
| title_short | Dissection of the genetic basis and molecular mechanism of ovule number per ovary in oilseed rape (Brassica napus L.) |
| title_sort | dissection of the genetic basis and molecular mechanism of ovule number per ovary in oilseed rape brassica napus l |
| topic | ovule number per ovary QTL mapping differentially expressed genes phytohormones candidate genes Brassica napus |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1489490/full |
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