A Label-Free Colorimetric Aptasensor for Flavokavain B Detection
Flavokavain B (FKB), a hepatotoxic chalcone from <i>Piper methysticum</i> (kava), has raised safety concerns due to its role in disrupting redox homeostasis and inducing apoptosis in hepatocytes. Conventional chromatographic methods for FKB detection, while sensitive, are costly and impr...
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MDPI AG
2025-01-01
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| Online Access: | https://www.mdpi.com/1424-8220/25/2/569 |
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| author | Sisi Ke Ningrui Wang Xingyu Chen Jiangwei Tian Jiwei Li Boyang Yu |
| author_facet | Sisi Ke Ningrui Wang Xingyu Chen Jiangwei Tian Jiwei Li Boyang Yu |
| author_sort | Sisi Ke |
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| description | Flavokavain B (FKB), a hepatotoxic chalcone from <i>Piper methysticum</i> (kava), has raised safety concerns due to its role in disrupting redox homeostasis and inducing apoptosis in hepatocytes. Conventional chromatographic methods for FKB detection, while sensitive, are costly and impractical for field applications. In this work, DNA aptamers were selected using the library-immobilized method and high-throughput sequencing. Three families of aptamers were obtained, and the best one named FKB-S showed a dissociation constant (K<sub>D</sub>) of 280 nM using microscale thermophoresis. To demonstrate its practical utility, a rapid and label-free colorimetric aptasensor was developed based on aptamer-induced gold nanoparticle aggregation. This assay achieved a detection limit of 150 nM (43.46 ng/mL) and provided results within 10 min. Compared to traditional chromatographic methods, the aptasensor offers a simple, cost-effective, and equipment-free approach for on-site FKB detection, making it a promising tool for the quality control and safety monitoring of kava-based products in diverse environments. |
| format | Article |
| id | doaj-art-45534ee1282640179fdde589894c00b2 |
| institution | Kabale University |
| issn | 1424-8220 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj-art-45534ee1282640179fdde589894c00b22025-01-24T13:49:22ZengMDPI AGSensors1424-82202025-01-0125256910.3390/s25020569A Label-Free Colorimetric Aptasensor for Flavokavain B DetectionSisi Ke0Ningrui Wang1Xingyu Chen2Jiangwei Tian3Jiwei Li4Boyang Yu5Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, ChinaSchool of Laboratory Medicine, Nanchang Medical College, Nanchang 330052, ChinaJiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, ChinaJiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, ChinaJiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, ChinaJiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, ChinaFlavokavain B (FKB), a hepatotoxic chalcone from <i>Piper methysticum</i> (kava), has raised safety concerns due to its role in disrupting redox homeostasis and inducing apoptosis in hepatocytes. Conventional chromatographic methods for FKB detection, while sensitive, are costly and impractical for field applications. In this work, DNA aptamers were selected using the library-immobilized method and high-throughput sequencing. Three families of aptamers were obtained, and the best one named FKB-S showed a dissociation constant (K<sub>D</sub>) of 280 nM using microscale thermophoresis. To demonstrate its practical utility, a rapid and label-free colorimetric aptasensor was developed based on aptamer-induced gold nanoparticle aggregation. This assay achieved a detection limit of 150 nM (43.46 ng/mL) and provided results within 10 min. Compared to traditional chromatographic methods, the aptasensor offers a simple, cost-effective, and equipment-free approach for on-site FKB detection, making it a promising tool for the quality control and safety monitoring of kava-based products in diverse environments.https://www.mdpi.com/1424-8220/25/2/569flavokavain Baptameraptasensorgold nanoparticlecolorimetric assay |
| spellingShingle | Sisi Ke Ningrui Wang Xingyu Chen Jiangwei Tian Jiwei Li Boyang Yu A Label-Free Colorimetric Aptasensor for Flavokavain B Detection Sensors flavokavain B aptamer aptasensor gold nanoparticle colorimetric assay |
| title | A Label-Free Colorimetric Aptasensor for Flavokavain B Detection |
| title_full | A Label-Free Colorimetric Aptasensor for Flavokavain B Detection |
| title_fullStr | A Label-Free Colorimetric Aptasensor for Flavokavain B Detection |
| title_full_unstemmed | A Label-Free Colorimetric Aptasensor for Flavokavain B Detection |
| title_short | A Label-Free Colorimetric Aptasensor for Flavokavain B Detection |
| title_sort | label free colorimetric aptasensor for flavokavain b detection |
| topic | flavokavain B aptamer aptasensor gold nanoparticle colorimetric assay |
| url | https://www.mdpi.com/1424-8220/25/2/569 |
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