Random Mutagenesis of the Aspergillus oryzae Genome Results in Fungal Antibacterial Activity
Multidrug-resistant bacteria cause severe infections in hospitals and communities. Development of new drugs to combat resistant microorganisms is needed. Natural products of microbial origin are the source of most currently available antibiotics. We hypothesized that random mutagenesis of Aspergill...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2013/901697 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832555526829899776 |
|---|---|
| author | Cory A. Leonard Stacy D. Brown J. Russell Hayman |
| author_facet | Cory A. Leonard Stacy D. Brown J. Russell Hayman |
| author_sort | Cory A. Leonard |
| collection | DOAJ |
| description | Multidrug-resistant bacteria cause severe infections in hospitals and communities. Development of new drugs to combat resistant microorganisms is needed. Natural products of microbial origin are the source of most currently available antibiotics. We hypothesized that random mutagenesis of Aspergillus oryzae would result in secretion of antibacterial compounds. To address this hypothesis, we developed a screen to identify individual A. oryzae mutants that inhibit the growth of Methicillin-resistant Staphylococcus aureus (MRSA) in vitro. To randomly generate A. oryzae mutant strains, spores were treated with ethyl methanesulfonate (EMS). Over 3000 EMS-treated A. oryzae cultures were tested in the screen, and one isolate, CAL220, exhibited altered morphology and antibacterial activity. Culture supernatant from this isolate showed antibacterial activity against Methicillin-sensitive Staphylococcus aureus, MRSA, and Pseudomonas aeruginosa, but not Klebsiella pneumonia or Proteus vulgaris. The results of this study support our hypothesis and suggest that the screen used is sufficient and appropriate to detect secreted antibacterial fungal compounds resulting from mutagenesis of A. oryzae. Because the genome of A. oryzae has been sequenced and systems are available for genetic transformation of this organism, targeted as well as random mutations may be introduced to facilitate the discovery of novel antibacterial compounds using this system. |
| format | Article |
| id | doaj-art-454c9eee7715469f851106c91eab0a07 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-454c9eee7715469f851106c91eab0a072025-02-03T05:48:01ZengWileyInternational Journal of Microbiology1687-918X1687-91982013-01-01201310.1155/2013/901697901697Random Mutagenesis of the Aspergillus oryzae Genome Results in Fungal Antibacterial ActivityCory A. Leonard0Stacy D. Brown1J. Russell Hayman2Department of Biomedical Sciences, James H. Quillen College of Medicine, East Tennessee State University, P.O. Box 70577, Johnson City, TN 37614, USADepartment of Pharmaceutical Sciences, Bill Gatton College of Pharmacy, East Tennessee State University, P.O. Box 70436, Johnson City, TN 37614, USADepartment of Biomedical Sciences, James H. Quillen College of Medicine, East Tennessee State University, P.O. Box 70577, Johnson City, TN 37614, USAMultidrug-resistant bacteria cause severe infections in hospitals and communities. Development of new drugs to combat resistant microorganisms is needed. Natural products of microbial origin are the source of most currently available antibiotics. We hypothesized that random mutagenesis of Aspergillus oryzae would result in secretion of antibacterial compounds. To address this hypothesis, we developed a screen to identify individual A. oryzae mutants that inhibit the growth of Methicillin-resistant Staphylococcus aureus (MRSA) in vitro. To randomly generate A. oryzae mutant strains, spores were treated with ethyl methanesulfonate (EMS). Over 3000 EMS-treated A. oryzae cultures were tested in the screen, and one isolate, CAL220, exhibited altered morphology and antibacterial activity. Culture supernatant from this isolate showed antibacterial activity against Methicillin-sensitive Staphylococcus aureus, MRSA, and Pseudomonas aeruginosa, but not Klebsiella pneumonia or Proteus vulgaris. The results of this study support our hypothesis and suggest that the screen used is sufficient and appropriate to detect secreted antibacterial fungal compounds resulting from mutagenesis of A. oryzae. Because the genome of A. oryzae has been sequenced and systems are available for genetic transformation of this organism, targeted as well as random mutations may be introduced to facilitate the discovery of novel antibacterial compounds using this system.http://dx.doi.org/10.1155/2013/901697 |
| spellingShingle | Cory A. Leonard Stacy D. Brown J. Russell Hayman Random Mutagenesis of the Aspergillus oryzae Genome Results in Fungal Antibacterial Activity International Journal of Microbiology |
| title | Random Mutagenesis of the Aspergillus oryzae Genome Results in Fungal Antibacterial Activity |
| title_full | Random Mutagenesis of the Aspergillus oryzae Genome Results in Fungal Antibacterial Activity |
| title_fullStr | Random Mutagenesis of the Aspergillus oryzae Genome Results in Fungal Antibacterial Activity |
| title_full_unstemmed | Random Mutagenesis of the Aspergillus oryzae Genome Results in Fungal Antibacterial Activity |
| title_short | Random Mutagenesis of the Aspergillus oryzae Genome Results in Fungal Antibacterial Activity |
| title_sort | random mutagenesis of the aspergillus oryzae genome results in fungal antibacterial activity |
| url | http://dx.doi.org/10.1155/2013/901697 |
| work_keys_str_mv | AT coryaleonard randommutagenesisoftheaspergillusoryzaegenomeresultsinfungalantibacterialactivity AT stacydbrown randommutagenesisoftheaspergillusoryzaegenomeresultsinfungalantibacterialactivity AT jrussellhayman randommutagenesisoftheaspergillusoryzaegenomeresultsinfungalantibacterialactivity |