Antioxidant and Anti-Obesity Properties of Acidic and Alkaline Seaweed Extracts Adjusted to Different pH Levels
This research examined antioxidant and anti-obesity effects of <i>Palmaria palmata</i> extracts obtained through acidic or alkaline treatments and subsequent pH adjustments. After two rounds of acidic or alkaline extraction, the extracts were separated from biomass and adjusted to differ...
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MDPI AG
2025-01-01
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| author | Sakhi Ghelichi Mona Hajfathalian Sara Falcione Charlotte Jacobsen |
| author_facet | Sakhi Ghelichi Mona Hajfathalian Sara Falcione Charlotte Jacobsen |
| author_sort | Sakhi Ghelichi |
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| description | This research examined antioxidant and anti-obesity effects of <i>Palmaria palmata</i> extracts obtained through acidic or alkaline treatments and subsequent pH adjustments. After two rounds of acidic or alkaline extraction, the extracts were separated from biomass and adjusted to different pH values: for acidic extracts, pH 3 (no adjustment), pH 6, pH 9, and pH 12; for alkaline extracts, pH 12 (no adjustment), pH 9, pH 6, and pH 3. The findings revealed that extraction medium as well as subsequent pH adjustments significantly influenced composition of the extracts in terms of protein content and recovery, amino acids, and phenolic compounds (<i>p</i> < 0.05). Acidic conditions produced extracts with potent radical scavenging, especially at pH 6 (IC<sub>50</sub> = 0.30 ± 0.04 mg.mL<sup>−1</sup>), while alkaline conditions favored metal chelating, with the highest Fe<sup>2+</sup> chelation at pH 12 (IC<sub>50</sub> = 0.65 ± 0.03 mg.mL<sup>−1</sup>). Moreover, extracts showed inhibitory activities against porcine pancreatic lipase and α-amylase, with the acidic extract at pH 9 showing the best anti-obesity properties (IC<sub>50</sub> = 5.38 ± 0.34 mg.mL<sup>−1</sup> for lipase and IC<sub>50</sub> = 5.79 ± 0.30 mg.mL<sup>−1</sup> for α-amylase). However, the highest α-amylase activity was in the alkaline extract at pH 12 (IC<sub>50</sub> = 3.05 ± 0.66 mg.mL<sup>−1</sup>). In conclusion, adjusting the pH of seaweed extracts notably influences their bioactive properties, likely due to changes in the reactivity and interactions of bioactive compounds such as peptides, carbohydrates, and polyphenols. |
| format | Article |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Marine Drugs |
| spelling | doaj-art-45281f5d27df497cbec0b2d6badbe2082025-01-24T13:39:34ZengMDPI AGMarine Drugs1660-33972025-01-012313510.3390/md23010035Antioxidant and Anti-Obesity Properties of Acidic and Alkaline Seaweed Extracts Adjusted to Different pH LevelsSakhi Ghelichi0Mona Hajfathalian1Sara Falcione2Charlotte Jacobsen3National Food Institute, Technical University of Denmark, 2800 Kongens Lyngby, DenmarkNational Food Institute, Technical University of Denmark, 2800 Kongens Lyngby, DenmarkDepartment of Health Technology, Technical University of Denmark, 2800 Kongens Lyngby, DenmarkNational Food Institute, Technical University of Denmark, 2800 Kongens Lyngby, DenmarkThis research examined antioxidant and anti-obesity effects of <i>Palmaria palmata</i> extracts obtained through acidic or alkaline treatments and subsequent pH adjustments. After two rounds of acidic or alkaline extraction, the extracts were separated from biomass and adjusted to different pH values: for acidic extracts, pH 3 (no adjustment), pH 6, pH 9, and pH 12; for alkaline extracts, pH 12 (no adjustment), pH 9, pH 6, and pH 3. The findings revealed that extraction medium as well as subsequent pH adjustments significantly influenced composition of the extracts in terms of protein content and recovery, amino acids, and phenolic compounds (<i>p</i> < 0.05). Acidic conditions produced extracts with potent radical scavenging, especially at pH 6 (IC<sub>50</sub> = 0.30 ± 0.04 mg.mL<sup>−1</sup>), while alkaline conditions favored metal chelating, with the highest Fe<sup>2+</sup> chelation at pH 12 (IC<sub>50</sub> = 0.65 ± 0.03 mg.mL<sup>−1</sup>). Moreover, extracts showed inhibitory activities against porcine pancreatic lipase and α-amylase, with the acidic extract at pH 9 showing the best anti-obesity properties (IC<sub>50</sub> = 5.38 ± 0.34 mg.mL<sup>−1</sup> for lipase and IC<sub>50</sub> = 5.79 ± 0.30 mg.mL<sup>−1</sup> for α-amylase). However, the highest α-amylase activity was in the alkaline extract at pH 12 (IC<sub>50</sub> = 3.05 ± 0.66 mg.mL<sup>−1</sup>). In conclusion, adjusting the pH of seaweed extracts notably influences their bioactive properties, likely due to changes in the reactivity and interactions of bioactive compounds such as peptides, carbohydrates, and polyphenols.https://www.mdpi.com/1660-3397/23/1/35extractionpH adjustmentprotein recovery<i>Palmaria palmata</i>radical scavengingchelating |
| spellingShingle | Sakhi Ghelichi Mona Hajfathalian Sara Falcione Charlotte Jacobsen Antioxidant and Anti-Obesity Properties of Acidic and Alkaline Seaweed Extracts Adjusted to Different pH Levels Marine Drugs extraction pH adjustment protein recovery <i>Palmaria palmata</i> radical scavenging chelating |
| title | Antioxidant and Anti-Obesity Properties of Acidic and Alkaline Seaweed Extracts Adjusted to Different pH Levels |
| title_full | Antioxidant and Anti-Obesity Properties of Acidic and Alkaline Seaweed Extracts Adjusted to Different pH Levels |
| title_fullStr | Antioxidant and Anti-Obesity Properties of Acidic and Alkaline Seaweed Extracts Adjusted to Different pH Levels |
| title_full_unstemmed | Antioxidant and Anti-Obesity Properties of Acidic and Alkaline Seaweed Extracts Adjusted to Different pH Levels |
| title_short | Antioxidant and Anti-Obesity Properties of Acidic and Alkaline Seaweed Extracts Adjusted to Different pH Levels |
| title_sort | antioxidant and anti obesity properties of acidic and alkaline seaweed extracts adjusted to different ph levels |
| topic | extraction pH adjustment protein recovery <i>Palmaria palmata</i> radical scavenging chelating |
| url | https://www.mdpi.com/1660-3397/23/1/35 |
| work_keys_str_mv | AT sakhighelichi antioxidantandantiobesitypropertiesofacidicandalkalineseaweedextractsadjustedtodifferentphlevels AT monahajfathalian antioxidantandantiobesitypropertiesofacidicandalkalineseaweedextractsadjustedtodifferentphlevels AT sarafalcione antioxidantandantiobesitypropertiesofacidicandalkalineseaweedextractsadjustedtodifferentphlevels AT charlottejacobsen antioxidantandantiobesitypropertiesofacidicandalkalineseaweedextractsadjustedtodifferentphlevels |