Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2: A Growth Factor to Induce Dentin Regeneration
We examined the effects of bone morphogenetic protein-2 (BMP-2) on growth, differentiation, and intracellular signaling pathways of odontoblast-like cells, KN-3 cells, to clarify molecular mechanisms of odontoblast differentiation during pulp regeneration process. After treatment with BMP-2, the cel...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2012/258469 |
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| author | Ayako Washio Chiaki Kitamura Takahiko Morotomi Masamichi Terashita Tatsuji Nishihara |
| author_facet | Ayako Washio Chiaki Kitamura Takahiko Morotomi Masamichi Terashita Tatsuji Nishihara |
| author_sort | Ayako Washio |
| collection | DOAJ |
| description | We examined the effects of bone morphogenetic protein-2 (BMP-2) on growth, differentiation, and intracellular signaling pathways of odontoblast-like cells, KN-3 cells, to clarify molecular mechanisms of odontoblast differentiation during pulp regeneration process. After treatment with BMP-2, the cell morphology, growth, alkaline phosphatase (ALP) activity, and the activation and expression of BMP-induced intracellular signaling molecules, such as Smad1/5/8 and Smad6/7, as well as activities of dentin sialoprotein (DSP) and dentin matrix protein 1 (DMP1), were examined. BMP-2 had no effects on the morphology, growth, or ALP activity of KN-3 cells, whereas it induced the phosphorylation of Smad1/5/8 and expression of Smad6/7. BMP-2 also induced the expressions of DSP and DMP-1. Our results suggest that KN-3 cells may express an odontoblastic phenotype with the addition of BMP-2 through the activation of Smad signaling pathways. |
| format | Article |
| id | doaj-art-451affbdee6548d590802b9dbba60082 |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-451affbdee6548d590802b9dbba600822025-02-03T06:08:48ZengWileyInternational Journal of Dentistry1687-87281687-87362012-01-01201210.1155/2012/258469258469Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2: A Growth Factor to Induce Dentin RegenerationAyako Washio0Chiaki Kitamura1Takahiko Morotomi2Masamichi Terashita3Tatsuji Nishihara4Division of Pulp Biology, Operative Dentistry, and Endodontics, Department of Cariology and Periodontology, Kyushu Dental College, Kitakyushu, JapanDivision of Pulp Biology, Operative Dentistry, and Endodontics, Department of Cariology and Periodontology, Kyushu Dental College, Kitakyushu, JapanSection of Operative Dentistry and Endodontology, Department of Odontology, Fukuoka Dental College, Fukuoka, JapanDivision of Comprehensive Dentistry, Department of Clinical Communication and Practice, Kyushu Dental College, Kitakyushu, JapanDivision of Infections and Molecular Biology, Department of Health Promotion, Kyushu Dental College, Kitakyushu, JapanWe examined the effects of bone morphogenetic protein-2 (BMP-2) on growth, differentiation, and intracellular signaling pathways of odontoblast-like cells, KN-3 cells, to clarify molecular mechanisms of odontoblast differentiation during pulp regeneration process. After treatment with BMP-2, the cell morphology, growth, alkaline phosphatase (ALP) activity, and the activation and expression of BMP-induced intracellular signaling molecules, such as Smad1/5/8 and Smad6/7, as well as activities of dentin sialoprotein (DSP) and dentin matrix protein 1 (DMP1), were examined. BMP-2 had no effects on the morphology, growth, or ALP activity of KN-3 cells, whereas it induced the phosphorylation of Smad1/5/8 and expression of Smad6/7. BMP-2 also induced the expressions of DSP and DMP-1. Our results suggest that KN-3 cells may express an odontoblastic phenotype with the addition of BMP-2 through the activation of Smad signaling pathways.http://dx.doi.org/10.1155/2012/258469 |
| spellingShingle | Ayako Washio Chiaki Kitamura Takahiko Morotomi Masamichi Terashita Tatsuji Nishihara Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2: A Growth Factor to Induce Dentin Regeneration International Journal of Dentistry |
| title | Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2: A Growth Factor to Induce Dentin Regeneration |
| title_full | Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2: A Growth Factor to Induce Dentin Regeneration |
| title_fullStr | Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2: A Growth Factor to Induce Dentin Regeneration |
| title_full_unstemmed | Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2: A Growth Factor to Induce Dentin Regeneration |
| title_short | Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2: A Growth Factor to Induce Dentin Regeneration |
| title_sort | possible involvement of smad signaling pathways in induction of odontoblastic properties in kn 3 cells by bone morphogenetic protein 2 a growth factor to induce dentin regeneration |
| url | http://dx.doi.org/10.1155/2012/258469 |
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