Application of synbiotic with different probiotic doses to prevent vibriosis in humpback grouper
ABSTRACT The aim of this study was to evaluate the supplementation of synbiotic with different doses of probiotics to prevent vibriosis in humpback grouper (Cromileptes altivelis). Grouper (3.0±0.48 g) fed by control feeds (without supplementation of synbiotic) which were K(-) and K(+), treatment...
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Asosiasi Sains Akuakultur Indonesia
2015-05-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/jai/article/view/9372 |
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| author | Dwi Agung Saputra , Sukenda , Widanarni |
| author_facet | Dwi Agung Saputra , Sukenda , Widanarni |
| author_sort | Dwi Agung Saputra |
| collection | DOAJ |
| description | ABSTRACT
The aim of this study was to evaluate the supplementation of synbiotic with different doses of probiotics to prevent vibriosis in humpback grouper (Cromileptes altivelis). Grouper (3.0±0.48 g) fed by control feeds (without supplementation of synbiotic) which were K(-) and K(+), treatment feeds with supplementation of synbiotic with different doses of probiotic (probiotic 104 cfu/mL) 1% + prebiotic 2% (P1) v/w, (probiotic 106 cfu/mL) 1% + prebiotic 2% (P2) v/w, (probiotic 108 cfu/mL)1% + prebiotic 2% (P3) v/w) for 30 days. After a feeding trial period, there were observation of the bacterial counts in the fish intestine, the fish growth performance and immune response. Then all the grouper were challenged by Vibrio alginolyticus, except K (-). This study showed that survival, daily growth and food conversion ratio (FCR) of grouper in treatment P2 ((probiotic 106 cfu/mL) 1% + prebiotic 2%) and P3 (probiotic (108 cfu/mL) 1% + prebiotic 2%) were significantly better (P>0.05) than controls. The different doses of probiotic in synbiotic (probiotic 104 cfu/mL, probiotic 106 cfu/mL and probiotic 108 cfu/mL) provided better immune response than controls.
Keywords: synbiotic, Vibrio alginolyticus, Bacillus sp., Cromileptes altivelis
ABSTRAK
Tujuan penelitian ini adalah mengkaji pemberian sinbiotik dengan dosis probiotik berbeda untuk mencegah penyakit vibriosis pada ikan kerapu bebek (Cromileptes altivelis). Ikan kerapu dengan berat (3,0±0,48 g) diberikan pakan kontrol (tanpa penambahan sinbiotik) K(-) dan K(+), pakan perlakuan dengan penambahan sinbiotik dengan dosis probiotik berbeda: probiotik ((104 cfu/mL) 1% + prebiotik 2%) v/w (P1), ((probiotik 106 cfu/mL) 1% + prebiotik 2%) v/w (P2), dan ((probiotik 108 cfu/mL)1% + prebiotik 2%) v/w (P3) selama 30 hari. Setelah perlakuan pakan sinbiotik, dilakukan pengamatan terhadap jumlah total bakteri di usus, kinerja pertumbuhan dan respons imun. Kemudian ikan kerapu pada seluruh perlakuan, kecuali kontrol negatif (-) diberi uji tantang dengan Vibrio alginolyticus. Hasil penelitian menunjukkan bahwa sintasan, laju pertumbuhan harian (LPH), dan rasio konversi pakan (FCR) pada perlakuan P2 ((probiotik 106 cfu/mL) 1% + prebiotik 2%) dan P3 (probiotik (108 cfu/mL) 1% + prebiotik 2%) secara signifikan lebih baik (P<0,05) bila dibandingkan dengan kontrol. Perlakuan sinbiotik dengan dosis probiotik berbeda (probiotik 104 cfu/mL, probiotik 106 cfu/mL, dan probiotik 108 cfu/mL) juga memberikan respons imun yang lebih baik dibandingkan dengan kontrol.
Kata kunci : sinbiotik, Vibrio alginolyticus, Bacillus sp., Cromileptes altivelis
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| format | Article |
| id | doaj-art-4505c3ac3090412eaa6528708c34424e |
| institution | Kabale University |
| issn | 1412-5269 2354-6700 |
| language | English |
| publishDate | 2015-05-01 |
| publisher | Asosiasi Sains Akuakultur Indonesia |
| record_format | Article |
| series | Jurnal Akuakultur Indonesia |
| spelling | doaj-art-4505c3ac3090412eaa6528708c34424e2025-01-24T17:59:14ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002015-05-0112210.19027/jai.12.169-1777836Application of synbiotic with different probiotic doses to prevent vibriosis in humpback grouperDwi Agung Saputra0, Sukenda1, Widanarni2Sekolah Pascasarjana, Program Studi Ilmu Akuakultur, Institut Pertanian Bogor Kampus IPB Dramaga Bogor 16680Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor. Kampus IPB Dramaga Bogor 16680Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor. Kampus IPB Dramaga Bogor 16680ABSTRACT The aim of this study was to evaluate the supplementation of synbiotic with different doses of probiotics to prevent vibriosis in humpback grouper (Cromileptes altivelis). Grouper (3.0±0.48 g) fed by control feeds (without supplementation of synbiotic) which were K(-) and K(+), treatment feeds with supplementation of synbiotic with different doses of probiotic (probiotic 104 cfu/mL) 1% + prebiotic 2% (P1) v/w, (probiotic 106 cfu/mL) 1% + prebiotic 2% (P2) v/w, (probiotic 108 cfu/mL)1% + prebiotic 2% (P3) v/w) for 30 days. After a feeding trial period, there were observation of the bacterial counts in the fish intestine, the fish growth performance and immune response. Then all the grouper were challenged by Vibrio alginolyticus, except K (-). This study showed that survival, daily growth and food conversion ratio (FCR) of grouper in treatment P2 ((probiotic 106 cfu/mL) 1% + prebiotic 2%) and P3 (probiotic (108 cfu/mL) 1% + prebiotic 2%) were significantly better (P>0.05) than controls. The different doses of probiotic in synbiotic (probiotic 104 cfu/mL, probiotic 106 cfu/mL and probiotic 108 cfu/mL) provided better immune response than controls. Keywords: synbiotic, Vibrio alginolyticus, Bacillus sp., Cromileptes altivelis ABSTRAK Tujuan penelitian ini adalah mengkaji pemberian sinbiotik dengan dosis probiotik berbeda untuk mencegah penyakit vibriosis pada ikan kerapu bebek (Cromileptes altivelis). Ikan kerapu dengan berat (3,0±0,48 g) diberikan pakan kontrol (tanpa penambahan sinbiotik) K(-) dan K(+), pakan perlakuan dengan penambahan sinbiotik dengan dosis probiotik berbeda: probiotik ((104 cfu/mL) 1% + prebiotik 2%) v/w (P1), ((probiotik 106 cfu/mL) 1% + prebiotik 2%) v/w (P2), dan ((probiotik 108 cfu/mL)1% + prebiotik 2%) v/w (P3) selama 30 hari. Setelah perlakuan pakan sinbiotik, dilakukan pengamatan terhadap jumlah total bakteri di usus, kinerja pertumbuhan dan respons imun. Kemudian ikan kerapu pada seluruh perlakuan, kecuali kontrol negatif (-) diberi uji tantang dengan Vibrio alginolyticus. Hasil penelitian menunjukkan bahwa sintasan, laju pertumbuhan harian (LPH), dan rasio konversi pakan (FCR) pada perlakuan P2 ((probiotik 106 cfu/mL) 1% + prebiotik 2%) dan P3 (probiotik (108 cfu/mL) 1% + prebiotik 2%) secara signifikan lebih baik (P<0,05) bila dibandingkan dengan kontrol. Perlakuan sinbiotik dengan dosis probiotik berbeda (probiotik 104 cfu/mL, probiotik 106 cfu/mL, dan probiotik 108 cfu/mL) juga memberikan respons imun yang lebih baik dibandingkan dengan kontrol. Kata kunci : sinbiotik, Vibrio alginolyticus, Bacillus sp., Cromileptes altivelis https://journal.ipb.ac.id/index.php/jai/article/view/9372 |
| spellingShingle | Dwi Agung Saputra , Sukenda , Widanarni Application of synbiotic with different probiotic doses to prevent vibriosis in humpback grouper Jurnal Akuakultur Indonesia |
| title | Application of synbiotic with different probiotic doses to prevent vibriosis in humpback grouper |
| title_full | Application of synbiotic with different probiotic doses to prevent vibriosis in humpback grouper |
| title_fullStr | Application of synbiotic with different probiotic doses to prevent vibriosis in humpback grouper |
| title_full_unstemmed | Application of synbiotic with different probiotic doses to prevent vibriosis in humpback grouper |
| title_short | Application of synbiotic with different probiotic doses to prevent vibriosis in humpback grouper |
| title_sort | application of synbiotic with different probiotic doses to prevent vibriosis in humpback grouper |
| url | https://journal.ipb.ac.id/index.php/jai/article/view/9372 |
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