Cholinoceptor Activation Subserving the Effects of Interferon Gamma on the Contractility of Rat Ileum
Recombinant rat interferon γ stimulated the contractility of isolated rat ileum at doses of 4–12 units/ml. Muscarinic cholinoceptors were involved, as treatment of the tissue with atropine prevented the contractile response of the ileum. Furthermore, interferon γ increased the affinity of carbachol...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
1994-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935194000645 |
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| Summary: | Recombinant rat interferon γ stimulated the contractility of
isolated rat ileum at doses of 4–12 units/ml. Muscarinic
cholinoceptors were involved, as treatment of the tissue with
atropine prevented the contractile response of the ileum.
Furthermore, interferon γ increased the affinity of carbachol for
the cholinoceptors and did not change its maximum effect. Neurogenic
pathways were also involved since pretreatment of ileum with
hexamethonium, hemicholinium or tetrodotoxin impaired the
contractile effect of interferon γ. In contrast to the action of
exogenous carbachol, the effects of interferon γ are indirect. They
appear to involve a G protein regulating phosphoinositide turnover
and cytoskeletal structures since they could not be induced in ileum
strips that were pretreated with pertussis toxin, phospholipase C
inhibitors (2-nitro-carboxyphenyl, NN-diphenyl carbamate and
neomycin), cytochalasine B or colchicine. |
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| ISSN: | 0962-9351 1466-1861 |