Effects of blood contamination and the rostro-caudal gradient on the human cerebrospinal fluid proteome.
Over the last years there has been an increased focus on the importance of knowing the effect of pre-analytical influence on the proteomes under study, particularly in the field of biomarker discovery. We present three proteomics studies examining the effect of blood contamination and the rostro-cau...
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0090429&type=printable |
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| author | Elise Aasebø Jill Anette Opsahl Yngvild Bjørlykke Kjell-Morten Myhr Ann Cathrine Kroksveen Frode S Berven |
| author_facet | Elise Aasebø Jill Anette Opsahl Yngvild Bjørlykke Kjell-Morten Myhr Ann Cathrine Kroksveen Frode S Berven |
| author_sort | Elise Aasebø |
| collection | DOAJ |
| description | Over the last years there has been an increased focus on the importance of knowing the effect of pre-analytical influence on the proteomes under study, particularly in the field of biomarker discovery. We present three proteomics studies examining the effect of blood contamination and the rostro-caudal gradient (RCG) on the cerebrospinal fluid (CSF) proteome, in addition to plasma/CSF protein ratios. The studies showed that the central nervous system (CNS) derived proteins appeared to be unaffected by the RCG, while the plasma-derived proteins showed an increase in concentration towards the lumbar area. This implies that the concentration of the plasma-derived proteins in CSF will vary depending on the volume of CSF that is collected. In the CSF samples spiked with blood, 262 of 814 quantified proteins showed an abundance increase of more than 1.5 fold, while 403 proteins had a fold change of less than 1.2 and appeared to be unaffected by blood contamination. Proteins with a high plasma/CSF ratio appeared to give the largest effect on the CSF proteome upon blood contamination. The results give important background information on how factors like blood contamination, RCG and blood-CNS-barrier influences the CSF proteome. This information is particularly important in the field of biomarker discovery, but also for routine clinical measurements. The data from the blood contamination and RCG discovery studies have been deposited to the ProteomeXchange with identifier PXD000401. |
| format | Article |
| id | doaj-art-44e701ece97c4509b48f2d96173315e7 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-44e701ece97c4509b48f2d96173315e72025-08-20T02:15:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0193e9042910.1371/journal.pone.0090429Effects of blood contamination and the rostro-caudal gradient on the human cerebrospinal fluid proteome.Elise AasebøJill Anette OpsahlYngvild BjørlykkeKjell-Morten MyhrAnn Cathrine KroksveenFrode S BervenOver the last years there has been an increased focus on the importance of knowing the effect of pre-analytical influence on the proteomes under study, particularly in the field of biomarker discovery. We present three proteomics studies examining the effect of blood contamination and the rostro-caudal gradient (RCG) on the cerebrospinal fluid (CSF) proteome, in addition to plasma/CSF protein ratios. The studies showed that the central nervous system (CNS) derived proteins appeared to be unaffected by the RCG, while the plasma-derived proteins showed an increase in concentration towards the lumbar area. This implies that the concentration of the plasma-derived proteins in CSF will vary depending on the volume of CSF that is collected. In the CSF samples spiked with blood, 262 of 814 quantified proteins showed an abundance increase of more than 1.5 fold, while 403 proteins had a fold change of less than 1.2 and appeared to be unaffected by blood contamination. Proteins with a high plasma/CSF ratio appeared to give the largest effect on the CSF proteome upon blood contamination. The results give important background information on how factors like blood contamination, RCG and blood-CNS-barrier influences the CSF proteome. This information is particularly important in the field of biomarker discovery, but also for routine clinical measurements. The data from the blood contamination and RCG discovery studies have been deposited to the ProteomeXchange with identifier PXD000401.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0090429&type=printable |
| spellingShingle | Elise Aasebø Jill Anette Opsahl Yngvild Bjørlykke Kjell-Morten Myhr Ann Cathrine Kroksveen Frode S Berven Effects of blood contamination and the rostro-caudal gradient on the human cerebrospinal fluid proteome. PLoS ONE |
| title | Effects of blood contamination and the rostro-caudal gradient on the human cerebrospinal fluid proteome. |
| title_full | Effects of blood contamination and the rostro-caudal gradient on the human cerebrospinal fluid proteome. |
| title_fullStr | Effects of blood contamination and the rostro-caudal gradient on the human cerebrospinal fluid proteome. |
| title_full_unstemmed | Effects of blood contamination and the rostro-caudal gradient on the human cerebrospinal fluid proteome. |
| title_short | Effects of blood contamination and the rostro-caudal gradient on the human cerebrospinal fluid proteome. |
| title_sort | effects of blood contamination and the rostro caudal gradient on the human cerebrospinal fluid proteome |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0090429&type=printable |
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