The Plant-Derived Bauhinia bauhinioides Kallikrein Proteinase Inhibitor (rBbKI) Attenuates Elastase-Induced Emphysema in Mice
Background. Elastase mediates important oxidative actions during the development of chronic obstructive pulmonary disease (COPD). However, few resources for the inhibition of elastase have been investigated. Our study evaluated the ability of the recombinant plant derived Bauhinia bauhinioides Kalli...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/5346574 |
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| author | Bruno Tadeu Martins-Olivera Rafael Almeida-Reis Osmar Aparecido Theodoro-Júnior Leandro Vilela Oliva Natalia Neto dos Santos Nunes Clarice Rosa Olivo Marlon Vilela de Brito Carla Máximo Prado Edna Aparecida Leick Mílton de Arruda Martins Maria Luiza Vilela Oliva Renato Fraga Righetti Iolanda de Fátima Lopes Calvo Tibério |
| author_facet | Bruno Tadeu Martins-Olivera Rafael Almeida-Reis Osmar Aparecido Theodoro-Júnior Leandro Vilela Oliva Natalia Neto dos Santos Nunes Clarice Rosa Olivo Marlon Vilela de Brito Carla Máximo Prado Edna Aparecida Leick Mílton de Arruda Martins Maria Luiza Vilela Oliva Renato Fraga Righetti Iolanda de Fátima Lopes Calvo Tibério |
| author_sort | Bruno Tadeu Martins-Olivera |
| collection | DOAJ |
| description | Background. Elastase mediates important oxidative actions during the development of chronic obstructive pulmonary disease (COPD). However, few resources for the inhibition of elastase have been investigated. Our study evaluated the ability of the recombinant plant derived Bauhinia bauhinioides Kallikrein proteinase Inhibitor (rBbKI) to modulate elastase-induced pulmonary inflammation. Methods. C57Bl/6 mice were given intratracheal elastase (ELA group) or saline (SAL group) and were treated intraperitoneally with rBbKI (ELA-rBbKI and SAL-rBbKI groups). At day 28, the following analyses were performed: (I) lung mechanics, (II) exhaled nitric oxide (ENO), (III) bronchoalveolar lavage fluid (BALF), and (IV) lung immunohistochemical staining. Results. In addition to decreasing mechanical alterations and alveolar septum disruption, rBbKI reduced the number of cells in the BALF and decreased the cellular expression of TNF-α, MMP-9, MMP-12, TIMP-1, eNOS, and iNOS in airways and alveolar walls compared with the ELA group. rBbKI decreased the volume proportion of 8-iso-PGF2α, collagen, and elastic fibers in the airways and alveolar walls compared with the ELA group. A reduction in the number of MUC-5-positive cells in the airway walls was also observed. Conclusion. rBbKI reduced elastase-induced pulmonary inflammation and extracellular matrix remodeling. rBbKI may be a potential pharmacological tool for COPD treatment. |
| format | Article |
| id | doaj-art-44dcef4aab8249049b8f4f1f372f513e |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-44dcef4aab8249049b8f4f1f372f513e2025-02-03T01:32:05ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/53465745346574The Plant-Derived Bauhinia bauhinioides Kallikrein Proteinase Inhibitor (rBbKI) Attenuates Elastase-Induced Emphysema in MiceBruno Tadeu Martins-Olivera0Rafael Almeida-Reis1Osmar Aparecido Theodoro-Júnior2Leandro Vilela Oliva3Natalia Neto dos Santos Nunes4Clarice Rosa Olivo5Marlon Vilela de Brito6Carla Máximo Prado7Edna Aparecida Leick8Mílton de Arruda Martins9Maria Luiza Vilela Oliva10Renato Fraga Righetti11Iolanda de Fátima Lopes Calvo Tibério12Department of Clinical Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, SP, BrazilDepartment of Clinical Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, SP, BrazilDepartment of Clinical Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, SP, BrazilDepartment of Clinical Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, SP, BrazilDepartment of Biochemistry, Universidade Federal de São Paulo, 04044-020 São Paulo, SP, BrazilDepartment of Clinical Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, SP, BrazilDepartment of Biochemistry, Universidade Federal de São Paulo, 04044-020 São Paulo, SP, BrazilDepartment of Bioscience, Federal University of Sao Paulo, 11015-020 Santos, SP, BrazilDepartment of Clinical Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, SP, BrazilDepartment of Clinical Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, SP, BrazilDepartment of Biochemistry, Universidade Federal de São Paulo, 04044-020 São Paulo, SP, BrazilDepartment of Clinical Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, SP, BrazilDepartment of Clinical Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, SP, BrazilBackground. Elastase mediates important oxidative actions during the development of chronic obstructive pulmonary disease (COPD). However, few resources for the inhibition of elastase have been investigated. Our study evaluated the ability of the recombinant plant derived Bauhinia bauhinioides Kallikrein proteinase Inhibitor (rBbKI) to modulate elastase-induced pulmonary inflammation. Methods. C57Bl/6 mice were given intratracheal elastase (ELA group) or saline (SAL group) and were treated intraperitoneally with rBbKI (ELA-rBbKI and SAL-rBbKI groups). At day 28, the following analyses were performed: (I) lung mechanics, (II) exhaled nitric oxide (ENO), (III) bronchoalveolar lavage fluid (BALF), and (IV) lung immunohistochemical staining. Results. In addition to decreasing mechanical alterations and alveolar septum disruption, rBbKI reduced the number of cells in the BALF and decreased the cellular expression of TNF-α, MMP-9, MMP-12, TIMP-1, eNOS, and iNOS in airways and alveolar walls compared with the ELA group. rBbKI decreased the volume proportion of 8-iso-PGF2α, collagen, and elastic fibers in the airways and alveolar walls compared with the ELA group. A reduction in the number of MUC-5-positive cells in the airway walls was also observed. Conclusion. rBbKI reduced elastase-induced pulmonary inflammation and extracellular matrix remodeling. rBbKI may be a potential pharmacological tool for COPD treatment.http://dx.doi.org/10.1155/2016/5346574 |
| spellingShingle | Bruno Tadeu Martins-Olivera Rafael Almeida-Reis Osmar Aparecido Theodoro-Júnior Leandro Vilela Oliva Natalia Neto dos Santos Nunes Clarice Rosa Olivo Marlon Vilela de Brito Carla Máximo Prado Edna Aparecida Leick Mílton de Arruda Martins Maria Luiza Vilela Oliva Renato Fraga Righetti Iolanda de Fátima Lopes Calvo Tibério The Plant-Derived Bauhinia bauhinioides Kallikrein Proteinase Inhibitor (rBbKI) Attenuates Elastase-Induced Emphysema in Mice Mediators of Inflammation |
| title | The Plant-Derived Bauhinia bauhinioides Kallikrein Proteinase Inhibitor (rBbKI) Attenuates Elastase-Induced Emphysema in Mice |
| title_full | The Plant-Derived Bauhinia bauhinioides Kallikrein Proteinase Inhibitor (rBbKI) Attenuates Elastase-Induced Emphysema in Mice |
| title_fullStr | The Plant-Derived Bauhinia bauhinioides Kallikrein Proteinase Inhibitor (rBbKI) Attenuates Elastase-Induced Emphysema in Mice |
| title_full_unstemmed | The Plant-Derived Bauhinia bauhinioides Kallikrein Proteinase Inhibitor (rBbKI) Attenuates Elastase-Induced Emphysema in Mice |
| title_short | The Plant-Derived Bauhinia bauhinioides Kallikrein Proteinase Inhibitor (rBbKI) Attenuates Elastase-Induced Emphysema in Mice |
| title_sort | plant derived bauhinia bauhinioides kallikrein proteinase inhibitor rbbki attenuates elastase induced emphysema in mice |
| url | http://dx.doi.org/10.1155/2016/5346574 |
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