The Importance of Ubiquitination and Deubiquitination in Cellular Reprogramming
Ubiquitination of core stem cell transcription factors can directly affect stem cell maintenance and differentiation. Ubiquitination and deubiquitination must occur in a timely and well-coordinated manner to regulate the protein turnover of several stemness related proteins, resulting in optimal emb...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/6705927 |
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| author | Bharathi Suresh Junwon Lee Kye-Seong Kim Suresh Ramakrishna |
| author_facet | Bharathi Suresh Junwon Lee Kye-Seong Kim Suresh Ramakrishna |
| author_sort | Bharathi Suresh |
| collection | DOAJ |
| description | Ubiquitination of core stem cell transcription factors can directly affect stem cell maintenance and differentiation. Ubiquitination and deubiquitination must occur in a timely and well-coordinated manner to regulate the protein turnover of several stemness related proteins, resulting in optimal embryonic stem cell maintenance and differentiation. There are two switches: an E3 ubiquitin ligase enzyme that tags ubiquitin molecules to the target proteins for proteolysis and a second enzyme, the deubiquitinating enzyme (DUBs), that performs the opposite action, thereby preventing proteolysis. In order to maintain stemness and to allow for efficient differentiation, both ubiquitination and deubiquitination molecular switches must operate properly in a balanced manner. In this review, we have summarized the importance of the ubiquitination of core stem cell transcription factors, such as Oct3/4, c-Myc, Sox2, Klf4, Nanog, and LIN28, during cellular reprogramming. Furthermore, we emphasize the role of DUBs in regulating core stem cell transcriptional factors and their function in stem cell maintenance and differentiation. We also discuss the possibility of using DUBs, along with core transcription factors, to efficiently generate induced pluripotent stem cells. Our review provides a relatively new understanding regarding the importance of ubiquitination/deubiquitination of stem cell transcription factors for efficient cellular reprogramming. |
| format | Article |
| id | doaj-art-44d54cec543b4a5382bd2bb122d50fe6 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-44d54cec543b4a5382bd2bb122d50fe62025-02-03T07:23:51ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/67059276705927The Importance of Ubiquitination and Deubiquitination in Cellular ReprogrammingBharathi Suresh0Junwon Lee1Kye-Seong Kim2Suresh Ramakrishna3Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 133-791, Republic of KoreaDepartment of Physiology and Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Republic of KoreaGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul 133-791, Republic of KoreaGraduate School of Biomedical Science and Engineering, Hanyang University, Seoul 133-791, Republic of KoreaUbiquitination of core stem cell transcription factors can directly affect stem cell maintenance and differentiation. Ubiquitination and deubiquitination must occur in a timely and well-coordinated manner to regulate the protein turnover of several stemness related proteins, resulting in optimal embryonic stem cell maintenance and differentiation. There are two switches: an E3 ubiquitin ligase enzyme that tags ubiquitin molecules to the target proteins for proteolysis and a second enzyme, the deubiquitinating enzyme (DUBs), that performs the opposite action, thereby preventing proteolysis. In order to maintain stemness and to allow for efficient differentiation, both ubiquitination and deubiquitination molecular switches must operate properly in a balanced manner. In this review, we have summarized the importance of the ubiquitination of core stem cell transcription factors, such as Oct3/4, c-Myc, Sox2, Klf4, Nanog, and LIN28, during cellular reprogramming. Furthermore, we emphasize the role of DUBs in regulating core stem cell transcriptional factors and their function in stem cell maintenance and differentiation. We also discuss the possibility of using DUBs, along with core transcription factors, to efficiently generate induced pluripotent stem cells. Our review provides a relatively new understanding regarding the importance of ubiquitination/deubiquitination of stem cell transcription factors for efficient cellular reprogramming.http://dx.doi.org/10.1155/2016/6705927 |
| spellingShingle | Bharathi Suresh Junwon Lee Kye-Seong Kim Suresh Ramakrishna The Importance of Ubiquitination and Deubiquitination in Cellular Reprogramming Stem Cells International |
| title | The Importance of Ubiquitination and Deubiquitination in Cellular Reprogramming |
| title_full | The Importance of Ubiquitination and Deubiquitination in Cellular Reprogramming |
| title_fullStr | The Importance of Ubiquitination and Deubiquitination in Cellular Reprogramming |
| title_full_unstemmed | The Importance of Ubiquitination and Deubiquitination in Cellular Reprogramming |
| title_short | The Importance of Ubiquitination and Deubiquitination in Cellular Reprogramming |
| title_sort | importance of ubiquitination and deubiquitination in cellular reprogramming |
| url | http://dx.doi.org/10.1155/2016/6705927 |
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