Disulfidptosis-related gene SLC3A2: a novel prognostic biomarker in nasopharyngeal carcinoma and head and neck squamous cell carcinoma
IntroductionNasopharyngeal carcinoma (NPC), one of the most common malignancies of the head and neck, is characterised by a complex pathogenesis and an unfavourable prognosis. Recently, disulfidoptosis, a novel form of cell death, has been proposed. Several studies in recent years have extensively i...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2025.1451034/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832589832595963904 |
|---|---|
| author | Xinyi Zhang Yiqi Lin Liang Shi Aixia Zhai Chao Wu Qian-Ying Zhu |
| author_facet | Xinyi Zhang Yiqi Lin Liang Shi Aixia Zhai Chao Wu Qian-Ying Zhu |
| author_sort | Xinyi Zhang |
| collection | DOAJ |
| description | IntroductionNasopharyngeal carcinoma (NPC), one of the most common malignancies of the head and neck, is characterised by a complex pathogenesis and an unfavourable prognosis. Recently, disulfidoptosis, a novel form of cell death, has been proposed. Several studies in recent years have extensively investigated the function of the disulfidoptosis-related SLC7A11 gene in cancer, but the role of its partner protein, SLC3A2, remains unknown unclear in NPC.MethodsGEO database analysis confirmed SLC3A2's prognostic impact on nasopharyngeal carcinoma. ROC, Kaplan-Meier analyses, and stage-specific expression studies showed a strong correlation with poor HNSC prognosis. GO and KEGG analyses pinpointed relevant signaling pathways. In vitro, SLC3A2's influence on cell proliferation, migration, and invasion was evaluated through CCK8, wound healing, colony formation, transwell assays, and cell cycle analysis.ResultsIn this study, we identified the high expression of SLC3A2 in NPC and head and neck squamous cell carcinoma (HNSC) and analyzed its potential mechanism and correlation with patient prognosis. Furthermore, a negative relationship was found between the expression level of SLC3A2 and the extent of immune cell infiltration and immune checkpoint. Differentially expressed genes (DEGs) between the high and low SLC3A2 expression groups were primarily involved in cytokine-cytokine receptor interaction and immune response. Finally, in vitro experiments demonstrated that SLC3A2 stimulates tumor cell proliferation and migration. DiscussionIn conclusion, these results indicated a strong association between SLC3A2 and progression in both NPC and HNSC, suggesting it as a promising biomarker for predicting adverse prognosis in NPC and HNSC patients. |
| format | Article |
| id | doaj-art-44d529760cfb4ba69cd268f7530a72ad |
| institution | Kabale University |
| issn | 2234-943X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj-art-44d529760cfb4ba69cd268f7530a72ad2025-01-24T07:14:10ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2025-01-011510.3389/fonc.2025.14510341451034Disulfidptosis-related gene SLC3A2: a novel prognostic biomarker in nasopharyngeal carcinoma and head and neck squamous cell carcinomaXinyi Zhang0Yiqi Lin1Liang Shi2Aixia Zhai3Chao Wu4Qian-Ying Zhu5Department of Laboratory Medicine, The Eighth Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaDepartment of Endocrinology, The Eighth Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaDepartment of Laboratory Medicine, The Eighth Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaDepartment of Laboratory Medicine, The Eighth Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaDepartment of Laboratory Medicine, The Eighth Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaDepartment of Laboratory Medicine, The Eighth Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaIntroductionNasopharyngeal carcinoma (NPC), one of the most common malignancies of the head and neck, is characterised by a complex pathogenesis and an unfavourable prognosis. Recently, disulfidoptosis, a novel form of cell death, has been proposed. Several studies in recent years have extensively investigated the function of the disulfidoptosis-related SLC7A11 gene in cancer, but the role of its partner protein, SLC3A2, remains unknown unclear in NPC.MethodsGEO database analysis confirmed SLC3A2's prognostic impact on nasopharyngeal carcinoma. ROC, Kaplan-Meier analyses, and stage-specific expression studies showed a strong correlation with poor HNSC prognosis. GO and KEGG analyses pinpointed relevant signaling pathways. In vitro, SLC3A2's influence on cell proliferation, migration, and invasion was evaluated through CCK8, wound healing, colony formation, transwell assays, and cell cycle analysis.ResultsIn this study, we identified the high expression of SLC3A2 in NPC and head and neck squamous cell carcinoma (HNSC) and analyzed its potential mechanism and correlation with patient prognosis. Furthermore, a negative relationship was found between the expression level of SLC3A2 and the extent of immune cell infiltration and immune checkpoint. Differentially expressed genes (DEGs) between the high and low SLC3A2 expression groups were primarily involved in cytokine-cytokine receptor interaction and immune response. Finally, in vitro experiments demonstrated that SLC3A2 stimulates tumor cell proliferation and migration. DiscussionIn conclusion, these results indicated a strong association between SLC3A2 and progression in both NPC and HNSC, suggesting it as a promising biomarker for predicting adverse prognosis in NPC and HNSC patients.https://www.frontiersin.org/articles/10.3389/fonc.2025.1451034/fullnasopharyngeal carcinomahead and neck squamous cell carcinomaSLC3A2biomarkerdisulfidptosis |
| spellingShingle | Xinyi Zhang Yiqi Lin Liang Shi Aixia Zhai Chao Wu Qian-Ying Zhu Disulfidptosis-related gene SLC3A2: a novel prognostic biomarker in nasopharyngeal carcinoma and head and neck squamous cell carcinoma Frontiers in Oncology nasopharyngeal carcinoma head and neck squamous cell carcinoma SLC3A2 biomarker disulfidptosis |
| title | Disulfidptosis-related gene SLC3A2: a novel prognostic biomarker in nasopharyngeal carcinoma and head and neck squamous cell carcinoma |
| title_full | Disulfidptosis-related gene SLC3A2: a novel prognostic biomarker in nasopharyngeal carcinoma and head and neck squamous cell carcinoma |
| title_fullStr | Disulfidptosis-related gene SLC3A2: a novel prognostic biomarker in nasopharyngeal carcinoma and head and neck squamous cell carcinoma |
| title_full_unstemmed | Disulfidptosis-related gene SLC3A2: a novel prognostic biomarker in nasopharyngeal carcinoma and head and neck squamous cell carcinoma |
| title_short | Disulfidptosis-related gene SLC3A2: a novel prognostic biomarker in nasopharyngeal carcinoma and head and neck squamous cell carcinoma |
| title_sort | disulfidptosis related gene slc3a2 a novel prognostic biomarker in nasopharyngeal carcinoma and head and neck squamous cell carcinoma |
| topic | nasopharyngeal carcinoma head and neck squamous cell carcinoma SLC3A2 biomarker disulfidptosis |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2025.1451034/full |
| work_keys_str_mv | AT xinyizhang disulfidptosisrelatedgeneslc3a2anovelprognosticbiomarkerinnasopharyngealcarcinomaandheadandnecksquamouscellcarcinoma AT yiqilin disulfidptosisrelatedgeneslc3a2anovelprognosticbiomarkerinnasopharyngealcarcinomaandheadandnecksquamouscellcarcinoma AT liangshi disulfidptosisrelatedgeneslc3a2anovelprognosticbiomarkerinnasopharyngealcarcinomaandheadandnecksquamouscellcarcinoma AT aixiazhai disulfidptosisrelatedgeneslc3a2anovelprognosticbiomarkerinnasopharyngealcarcinomaandheadandnecksquamouscellcarcinoma AT chaowu disulfidptosisrelatedgeneslc3a2anovelprognosticbiomarkerinnasopharyngealcarcinomaandheadandnecksquamouscellcarcinoma AT qianyingzhu disulfidptosisrelatedgeneslc3a2anovelprognosticbiomarkerinnasopharyngealcarcinomaandheadandnecksquamouscellcarcinoma |