Actin Alpha 2 (ACTA2) Downregulation Inhibits Neural Stem Cell Migration through Rho GTPase Activation
Although neural stem cells (NSCs) could migrate towards lesions after central nervous system (CNS) injury, the migration ability always is restricted due to the disturbed composition and density of the adhesion ligands and extracellular matrix (ECM) gradient after CNS injury. To date, various method...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2020/4764012 |
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| author | Ji Zhang Xuheng Jiang Chao Zhang Jun Zhong Xuanyu Fang Huanhuan Li Fangke Xie Xiaofei Huang Xiaojun Zhang Quan Hu Hongfei Ge Anyong Yu |
| author_facet | Ji Zhang Xuheng Jiang Chao Zhang Jun Zhong Xuanyu Fang Huanhuan Li Fangke Xie Xiaofei Huang Xiaojun Zhang Quan Hu Hongfei Ge Anyong Yu |
| author_sort | Ji Zhang |
| collection | DOAJ |
| description | Although neural stem cells (NSCs) could migrate towards lesions after central nervous system (CNS) injury, the migration ability always is restricted due to the disturbed composition and density of the adhesion ligands and extracellular matrix (ECM) gradient after CNS injury. To date, various methods have been developed to enhance NSC migration and a number of factors, which are affecting NSC migration potential, have been identified. Here, primary NSCs were cultured and the expression of actin alpha 2 (ACTA2) in NSCs was determined using reverse transcription polymerase chain reaction (RT-PCR) and immunostaining. Next, the role of ACTA2 in regulating NSC migration and the potential mechanism was explored. Our results demonstrated that ACTA2 expressed in NSCs. Meanwhile, downregulated ACTA2 using siRNA inhibited NSC migration through hindering actin filament polymerization via increasing RhoA expression and decreasing Rac1 expression. The present study might enrich the basic knowledge of ACTA2 in NSC migration and open an avenue for enhancing NSC migration potential, subsequently providing an intervention target for functional recovery after CNS injury. |
| format | Article |
| id | doaj-art-44c8a81bf14b4e8ab39d3b2f6ca36b4e |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-44c8a81bf14b4e8ab39d3b2f6ca36b4e2025-02-03T05:54:26ZengWileyStem Cells International1687-966X1687-96782020-01-01202010.1155/2020/47640124764012Actin Alpha 2 (ACTA2) Downregulation Inhibits Neural Stem Cell Migration through Rho GTPase ActivationJi Zhang0Xuheng Jiang1Chao Zhang2Jun Zhong3Xuanyu Fang4Huanhuan Li5Fangke Xie6Xiaofei Huang7Xiaojun Zhang8Quan Hu9Hongfei Ge10Anyong Yu11Department of Emergency, Hospital of Zunyi Medical University, 563003 Zunyi, Guizhou, ChinaDepartment of Emergency, Hospital of Zunyi Medical University, 563003 Zunyi, Guizhou, ChinaDepartment of Neurosurgery and Key Laboratory of Neurotrauma, Southwest Hospital, Military Medical University (Army Medical University), 400038 Chongqing, ChinaDepartment of Neurosurgery and Key Laboratory of Neurotrauma, Southwest Hospital, Military Medical University (Army Medical University), 400038 Chongqing, ChinaDepartment of Neurosurgery and Key Laboratory of Neurotrauma, Southwest Hospital, Military Medical University (Army Medical University), 400038 Chongqing, ChinaDepartment of Neurosurgery and Key Laboratory of Neurotrauma, Southwest Hospital, Military Medical University (Army Medical University), 400038 Chongqing, ChinaDepartment of Emergency, Hospital of Zunyi Medical University, 563003 Zunyi, Guizhou, ChinaDepartment of Emergency, Hospital of Zunyi Medical University, 563003 Zunyi, Guizhou, ChinaDepartment of Emergency, Hospital of Zunyi Medical University, 563003 Zunyi, Guizhou, ChinaDepartment of Emergency, Hospital of Zunyi Medical University, 563003 Zunyi, Guizhou, ChinaDepartment of Neurosurgery and Key Laboratory of Neurotrauma, Southwest Hospital, Military Medical University (Army Medical University), 400038 Chongqing, ChinaDepartment of Emergency, Hospital of Zunyi Medical University, 563003 Zunyi, Guizhou, ChinaAlthough neural stem cells (NSCs) could migrate towards lesions after central nervous system (CNS) injury, the migration ability always is restricted due to the disturbed composition and density of the adhesion ligands and extracellular matrix (ECM) gradient after CNS injury. To date, various methods have been developed to enhance NSC migration and a number of factors, which are affecting NSC migration potential, have been identified. Here, primary NSCs were cultured and the expression of actin alpha 2 (ACTA2) in NSCs was determined using reverse transcription polymerase chain reaction (RT-PCR) and immunostaining. Next, the role of ACTA2 in regulating NSC migration and the potential mechanism was explored. Our results demonstrated that ACTA2 expressed in NSCs. Meanwhile, downregulated ACTA2 using siRNA inhibited NSC migration through hindering actin filament polymerization via increasing RhoA expression and decreasing Rac1 expression. The present study might enrich the basic knowledge of ACTA2 in NSC migration and open an avenue for enhancing NSC migration potential, subsequently providing an intervention target for functional recovery after CNS injury.http://dx.doi.org/10.1155/2020/4764012 |
| spellingShingle | Ji Zhang Xuheng Jiang Chao Zhang Jun Zhong Xuanyu Fang Huanhuan Li Fangke Xie Xiaofei Huang Xiaojun Zhang Quan Hu Hongfei Ge Anyong Yu Actin Alpha 2 (ACTA2) Downregulation Inhibits Neural Stem Cell Migration through Rho GTPase Activation Stem Cells International |
| title | Actin Alpha 2 (ACTA2) Downregulation Inhibits Neural Stem Cell Migration through Rho GTPase Activation |
| title_full | Actin Alpha 2 (ACTA2) Downregulation Inhibits Neural Stem Cell Migration through Rho GTPase Activation |
| title_fullStr | Actin Alpha 2 (ACTA2) Downregulation Inhibits Neural Stem Cell Migration through Rho GTPase Activation |
| title_full_unstemmed | Actin Alpha 2 (ACTA2) Downregulation Inhibits Neural Stem Cell Migration through Rho GTPase Activation |
| title_short | Actin Alpha 2 (ACTA2) Downregulation Inhibits Neural Stem Cell Migration through Rho GTPase Activation |
| title_sort | actin alpha 2 acta2 downregulation inhibits neural stem cell migration through rho gtpase activation |
| url | http://dx.doi.org/10.1155/2020/4764012 |
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