Protocol to identify small-molecule inhibitors against cancer drug resistance
Summary: Drug resistance has emerged as a critical challenge in clinical cancer treatment. Here, we present a high-throughput screening protocol to identify therapeutic small-molecule inhibitors against drug-resistant cancer cells. We detail the steps for constructing drug-resistant cell models, exe...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000115 |
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| author | Juan Wei She-Yu Zhang Xue-Yuan Zhou Yong Wei Hao-Ran Jia Qin Wu Weihong Tan |
| author_facet | Juan Wei She-Yu Zhang Xue-Yuan Zhou Yong Wei Hao-Ran Jia Qin Wu Weihong Tan |
| author_sort | Juan Wei |
| collection | DOAJ |
| description | Summary: Drug resistance has emerged as a critical challenge in clinical cancer treatment. Here, we present a high-throughput screening protocol to identify therapeutic small-molecule inhibitors against drug-resistant cancer cells. We detail the steps for constructing drug-resistant cell models, executing the chemical screening process, and performing data analysis and validation. This protocol facilitates the rapid identification of therapeutic strategies for different types of drug-resistant cancers and aids in studying mechanisms.For complete details on the use and execution of this protocol, please refer to Zhang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-44b9e0f914dc4fab9e388cf384a2ed74 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-44b9e0f914dc4fab9e388cf384a2ed742025-01-31T05:12:25ZengElsevierSTAR Protocols2666-16672025-03-0161103605Protocol to identify small-molecule inhibitors against cancer drug resistanceJuan Wei0She-Yu Zhang1Xue-Yuan Zhou2Yong Wei3Hao-Ran Jia4Qin Wu5Weihong Tan6School of Science, Tianjin University, Tianjin, ChinaSchool of Life Sciences, Tianjin University, Tianjin, ChinaHangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang, ChinaHangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang, ChinaInstitute of Molecular Medicine (IMM), Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Corresponding authorHangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang, China; Corresponding authorHangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang, China; Institute of Molecular Medicine (IMM), Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Corresponding authorSummary: Drug resistance has emerged as a critical challenge in clinical cancer treatment. Here, we present a high-throughput screening protocol to identify therapeutic small-molecule inhibitors against drug-resistant cancer cells. We detail the steps for constructing drug-resistant cell models, executing the chemical screening process, and performing data analysis and validation. This protocol facilitates the rapid identification of therapeutic strategies for different types of drug-resistant cancers and aids in studying mechanisms.For complete details on the use and execution of this protocol, please refer to Zhang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725000115cell-based assayscancerhigh-throughput screening |
| spellingShingle | Juan Wei She-Yu Zhang Xue-Yuan Zhou Yong Wei Hao-Ran Jia Qin Wu Weihong Tan Protocol to identify small-molecule inhibitors against cancer drug resistance STAR Protocols cell-based assays cancer high-throughput screening |
| title | Protocol to identify small-molecule inhibitors against cancer drug resistance |
| title_full | Protocol to identify small-molecule inhibitors against cancer drug resistance |
| title_fullStr | Protocol to identify small-molecule inhibitors against cancer drug resistance |
| title_full_unstemmed | Protocol to identify small-molecule inhibitors against cancer drug resistance |
| title_short | Protocol to identify small-molecule inhibitors against cancer drug resistance |
| title_sort | protocol to identify small molecule inhibitors against cancer drug resistance |
| topic | cell-based assays cancer high-throughput screening |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725000115 |
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