Graphene Oxide-Gold Star Construct on Triangular Electrodes for Alzheimer’s Disease Identification
Nanotechnology is playing a major role in the field of medical diagnosis, in particular with the biosensor and bioimaging. It improves the performance of the desired system dramatically by displaying higher selectivity and sensitivity. Carbon nanomaterial, gold nanostructure, magnetite nanoparticle,...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2021/6661799 |
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| author | Wenlong Chang Jing Zhao Lu Liu Xiaoming Xing Chao Zhang Huihong Meng Subash C. B. Gopinath Yonggang Liu |
| author_facet | Wenlong Chang Jing Zhao Lu Liu Xiaoming Xing Chao Zhang Huihong Meng Subash C. B. Gopinath Yonggang Liu |
| author_sort | Wenlong Chang |
| collection | DOAJ |
| description | Nanotechnology is playing a major role in the field of medical diagnosis, in particular with the biosensor and bioimaging. It improves the performance of the desired system dramatically by displaying higher selectivity and sensitivity. Carbon nanomaterial, gold nanostructure, magnetite nanoparticle, and silica substrate are the most popular nanomaterials greatly contributed to make the affordable and effective biosensor at low-cost. This research work is introducing a new sensing strategy with graphene oxide-constructed triangular electrodes to diagnose Alzheimer’s disease (AD). MicroRNA-137 (miRNA-137) was found as a suitable biomarker for AD, and the sensing method was established here to detect miRNA-137 on the complementary sequence. To enhance the immobilization of capture miRNA-137, gold nanostar (GNS) was conjugated with capture miRNA and immobilized on the GO-modified surface through an amine linker. This immobilization process enhanced the hybridization of the target and reaches the detection limit at 10 fM with the sensitivity of 1 fM on the linear curve with a regression coefficient of 0.9038. Further control sequences of miRNA-21 and single and triple base mismatched miRNA-137 did not show a significant response in current changes, indicating the specific miRNA-137 detection for diagnosing AD. |
| format | Article |
| id | doaj-art-44aaec65048e4530b178a8a7ec2d5e12 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-44aaec65048e4530b178a8a7ec2d5e122025-02-03T01:32:25ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732021-01-01202110.1155/2021/66617996661799Graphene Oxide-Gold Star Construct on Triangular Electrodes for Alzheimer’s Disease IdentificationWenlong Chang0Jing Zhao1Lu Liu2Xiaoming Xing3Chao Zhang4Huihong Meng5Subash C. B. Gopinath6Yonggang Liu7Endocrine Laboratory, Baoding No.1 Central Hospital, Baoding, Hebei 071000, ChinaDepartment of First Neurology, Baoding No.1 Central Hospital, Baoding, Hebei 071000, ChinaDepartment of Clinical Psychology, Baoding No.1 Central Hospital, Baoding, Hebei 071000, ChinaDepartment of First Neurology, Baoding No.1 Central Hospital, Baoding, Hebei 071000, ChinaDepartment of First Neurology, Baoding No.1 Central Hospital, Baoding, Hebei 071000, ChinaDepartment of First Neurology, Baoding No.1 Central Hospital, Baoding, Hebei 071000, ChinaFaculty of Chemical Engineering Technology, Universiti Malaysia Perlis (UniMAP), Arau 02600, Perlis, MalaysiaDepartment of First Neurology, Baoding No.1 Central Hospital, Baoding, Hebei 071000, ChinaNanotechnology is playing a major role in the field of medical diagnosis, in particular with the biosensor and bioimaging. It improves the performance of the desired system dramatically by displaying higher selectivity and sensitivity. Carbon nanomaterial, gold nanostructure, magnetite nanoparticle, and silica substrate are the most popular nanomaterials greatly contributed to make the affordable and effective biosensor at low-cost. This research work is introducing a new sensing strategy with graphene oxide-constructed triangular electrodes to diagnose Alzheimer’s disease (AD). MicroRNA-137 (miRNA-137) was found as a suitable biomarker for AD, and the sensing method was established here to detect miRNA-137 on the complementary sequence. To enhance the immobilization of capture miRNA-137, gold nanostar (GNS) was conjugated with capture miRNA and immobilized on the GO-modified surface through an amine linker. This immobilization process enhanced the hybridization of the target and reaches the detection limit at 10 fM with the sensitivity of 1 fM on the linear curve with a regression coefficient of 0.9038. Further control sequences of miRNA-21 and single and triple base mismatched miRNA-137 did not show a significant response in current changes, indicating the specific miRNA-137 detection for diagnosing AD.http://dx.doi.org/10.1155/2021/6661799 |
| spellingShingle | Wenlong Chang Jing Zhao Lu Liu Xiaoming Xing Chao Zhang Huihong Meng Subash C. B. Gopinath Yonggang Liu Graphene Oxide-Gold Star Construct on Triangular Electrodes for Alzheimer’s Disease Identification Journal of Analytical Methods in Chemistry |
| title | Graphene Oxide-Gold Star Construct on Triangular Electrodes for Alzheimer’s Disease Identification |
| title_full | Graphene Oxide-Gold Star Construct on Triangular Electrodes for Alzheimer’s Disease Identification |
| title_fullStr | Graphene Oxide-Gold Star Construct on Triangular Electrodes for Alzheimer’s Disease Identification |
| title_full_unstemmed | Graphene Oxide-Gold Star Construct on Triangular Electrodes for Alzheimer’s Disease Identification |
| title_short | Graphene Oxide-Gold Star Construct on Triangular Electrodes for Alzheimer’s Disease Identification |
| title_sort | graphene oxide gold star construct on triangular electrodes for alzheimer s disease identification |
| url | http://dx.doi.org/10.1155/2021/6661799 |
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