Effects of Electroacupuncture at Governor Vessel Acupoints on Neurotrophin-3 in Rats with Experimental Spinal Cord Injury
In an effort to explore new, noninvasive treatment options for spinal cord injuries (SCI), this study investigated the effects of electroacupuncture (EA) for SCI rat models. SCI was induced by a modified Allen’s weight-drop method. We investigated the response of EA at Dazhui (GV 14) and Mingmen (GV...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2016/2371875 |
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| author | Yu-ping Mo Hai-jiang Yao Wei Lv Liang-yu Song Hong-tao Song Xiao-chen Yuan Ying-qiu Mao Quan-kai Jing Su-hua Shi Zhi-gang Li |
| author_facet | Yu-ping Mo Hai-jiang Yao Wei Lv Liang-yu Song Hong-tao Song Xiao-chen Yuan Ying-qiu Mao Quan-kai Jing Su-hua Shi Zhi-gang Li |
| author_sort | Yu-ping Mo |
| collection | DOAJ |
| description | In an effort to explore new, noninvasive treatment options for spinal cord injuries (SCI), this study investigated the effects of electroacupuncture (EA) for SCI rat models. SCI was induced by a modified Allen’s weight-drop method. We investigated the response of EA at Dazhui (GV 14) and Mingmen (GV 4) acupoints to understand the effects and mechanisms of EA in neuroprotection and neuronal function recovery after SCI. BBB testing was used to detect motor function of rats’ hind limbs among groups, and EA was shown to promote the recovery of SCI rats’ motor function. Nissl staining showed a restored neural morphology and an increase in the quantity of neurons after EA. Also, the antiapoptosis role was exposed by TUNEL staining. Western blotting analysis was used to determine the protein expression of neurotrophin-3 (NT-3) in spinal cord tissue. Compared to the sham group, the expression levels of NT-3 were significantly decreased and EA was shown to upregulate the expression of NT-3. The present study suggests that the role of EA in neuroprotection and dorsal neuronal function recovery after SCI in rats, especially EA stimulation at GV 14 and GV 4, can greatly promote neuronal function recovery, which may result from upregulating the expression of NT-3. |
| format | Article |
| id | doaj-art-44aa96840bd6430b894da5badac5caa3 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-44aa96840bd6430b894da5badac5caa32025-02-03T06:44:31ZengWileyNeural Plasticity2090-59041687-54432016-01-01201610.1155/2016/23718752371875Effects of Electroacupuncture at Governor Vessel Acupoints on Neurotrophin-3 in Rats with Experimental Spinal Cord InjuryYu-ping Mo0Hai-jiang Yao1Wei Lv2Liang-yu Song3Hong-tao Song4Xiao-chen Yuan5Ying-qiu Mao6Quan-kai Jing7Su-hua Shi8Zhi-gang Li9Department of Rehabilitation, The Third People’s Hospital of Shenzhen, No. 29 Bulan Road, Longgang District, Shenzhen 518112, ChinaSchool of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, No. 11 North Third Ring Road East, Chaoyang District, Beijing 100029, ChinaSchool of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, No. 11 North Third Ring Road East, Chaoyang District, Beijing 100029, ChinaSchool of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, No. 11 North Third Ring Road East, Chaoyang District, Beijing 100029, ChinaDepartment of Traditional Chinese Medicine, Inner Mongolia People’s Hospital, No. 20 Zhao Wudaroad, Hohhot, Inner Mongolia Autonomous Region 010017, ChinaInstitute of Microcirculation, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 5 Dong Dan San Tiao Street, Dongcheng District, Beijing 100005, ChinaResearch and Experimental Center, Beijing University of Chinese Medicine, No. 11 North Third Ring Road East, Chaoyang District, Beijing 100029, ChinaSchool of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, No. 11 North Third Ring Road East, Chaoyang District, Beijing 100029, ChinaDepartment of Rehabilitation, The Third Affiliated Hospital of Beijing University of Chinese Medicine, No. 51 An Wai Xiao Guan Street, Chaoyang District, Beijing 100029, ChinaSchool of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, No. 11 North Third Ring Road East, Chaoyang District, Beijing 100029, ChinaIn an effort to explore new, noninvasive treatment options for spinal cord injuries (SCI), this study investigated the effects of electroacupuncture (EA) for SCI rat models. SCI was induced by a modified Allen’s weight-drop method. We investigated the response of EA at Dazhui (GV 14) and Mingmen (GV 4) acupoints to understand the effects and mechanisms of EA in neuroprotection and neuronal function recovery after SCI. BBB testing was used to detect motor function of rats’ hind limbs among groups, and EA was shown to promote the recovery of SCI rats’ motor function. Nissl staining showed a restored neural morphology and an increase in the quantity of neurons after EA. Also, the antiapoptosis role was exposed by TUNEL staining. Western blotting analysis was used to determine the protein expression of neurotrophin-3 (NT-3) in spinal cord tissue. Compared to the sham group, the expression levels of NT-3 were significantly decreased and EA was shown to upregulate the expression of NT-3. The present study suggests that the role of EA in neuroprotection and dorsal neuronal function recovery after SCI in rats, especially EA stimulation at GV 14 and GV 4, can greatly promote neuronal function recovery, which may result from upregulating the expression of NT-3.http://dx.doi.org/10.1155/2016/2371875 |
| spellingShingle | Yu-ping Mo Hai-jiang Yao Wei Lv Liang-yu Song Hong-tao Song Xiao-chen Yuan Ying-qiu Mao Quan-kai Jing Su-hua Shi Zhi-gang Li Effects of Electroacupuncture at Governor Vessel Acupoints on Neurotrophin-3 in Rats with Experimental Spinal Cord Injury Neural Plasticity |
| title | Effects of Electroacupuncture at Governor Vessel Acupoints on Neurotrophin-3 in Rats with Experimental Spinal Cord Injury |
| title_full | Effects of Electroacupuncture at Governor Vessel Acupoints on Neurotrophin-3 in Rats with Experimental Spinal Cord Injury |
| title_fullStr | Effects of Electroacupuncture at Governor Vessel Acupoints on Neurotrophin-3 in Rats with Experimental Spinal Cord Injury |
| title_full_unstemmed | Effects of Electroacupuncture at Governor Vessel Acupoints on Neurotrophin-3 in Rats with Experimental Spinal Cord Injury |
| title_short | Effects of Electroacupuncture at Governor Vessel Acupoints on Neurotrophin-3 in Rats with Experimental Spinal Cord Injury |
| title_sort | effects of electroacupuncture at governor vessel acupoints on neurotrophin 3 in rats with experimental spinal cord injury |
| url | http://dx.doi.org/10.1155/2016/2371875 |
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