Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit
The present work is a pioneer study specifically addressing the aquaporin transcripts in sugarcane transcriptomes. Representatives of the four aquaporin subfamilies (PIP, TIP, SIP, and NIP), already described for higher plants, were identified. Forty-two distinct aquaporin isoforms were expressed in...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.1155/2013/763945 |
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| author | Manassés Daniel da Silva Roberta Lane de Oliveira Silva José Ribamar Costa Ferreira Neto Ana Carolina Ribeiro Guimarães Daniela Truffi Veiga Sabrina Moutinho Chabregas William Lee Burnquist Günter Kahl Ana Maria Benko-Iseppon Ederson Akio Kido |
| author_facet | Manassés Daniel da Silva Roberta Lane de Oliveira Silva José Ribamar Costa Ferreira Neto Ana Carolina Ribeiro Guimarães Daniela Truffi Veiga Sabrina Moutinho Chabregas William Lee Burnquist Günter Kahl Ana Maria Benko-Iseppon Ederson Akio Kido |
| author_sort | Manassés Daniel da Silva |
| collection | DOAJ |
| description | The present work is a pioneer study specifically addressing the aquaporin transcripts in sugarcane transcriptomes. Representatives of the four aquaporin subfamilies (PIP, TIP, SIP, and NIP), already described for higher plants, were identified. Forty-two distinct aquaporin isoforms were expressed in four HT-SuperSAGE libraries from sugarcane roots of drought-tolerant and -sensitive genotypes, respectively. At least 10 different potential aquaporin isoform targets and their respective unitags were considered to be promising for future studies and especially for the development of molecular markers for plant breeding. From those 10 isoforms, four (SoPIP2-4, SoPIP2-6, OsPIP2-4, and SsPIP1-1) showed distinct responses towards drought, with divergent expressions between the bulks from tolerant and sensitive genotypes, when they were compared under normal and stress conditions. Two targets (SsPIP1-1 and SoPIP1-3/PIP1-4) were selected for validation via RT-qPCR and their expression patterns as detected by HT-SuperSAGE were confirmed. The employed validation strategy revealed that different genotypes share the same tolerant or sensitive phenotype, respectively, but may use different routes for stress acclimation, indicating the aquaporin transcription in sugarcane to be potentially genotype-specific. |
| format | Article |
| id | doaj-art-449c914db96e4dfab62c164b6918f4a6 |
| institution | Kabale University |
| issn | 2090-0201 2090-021X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nucleic Acids |
| spelling | doaj-art-449c914db96e4dfab62c164b6918f4a62025-02-03T01:21:23ZengWileyJournal of Nucleic Acids2090-02012090-021X2013-01-01201310.1155/2013/763945763945Expression Analysis of Sugarcane Aquaporin Genes under Water DeficitManassés Daniel da Silva0Roberta Lane de Oliveira Silva1José Ribamar Costa Ferreira Neto2Ana Carolina Ribeiro Guimarães3Daniela Truffi Veiga4Sabrina Moutinho Chabregas5William Lee Burnquist6Günter Kahl7Ana Maria Benko-Iseppon8Ederson Akio Kido9Department of Genetics, Federal University of Pernambuco (UFPE), 50670-901 Recife, PE, BrazilDepartment of Genetics, Federal University of Pernambuco (UFPE), 50670-901 Recife, PE, BrazilDepartment of Genetics, Federal University of Pernambuco (UFPE), 50670-901 Recife, PE, BrazilBiotechnology Division, Sugarcane Technology Center (CTC), 13400-970 Piracicaba, SP, BrazilBiotechnology Division, Sugarcane Technology Center (CTC), 13400-970 Piracicaba, SP, BrazilBiotechnology Division, Sugarcane Technology Center (CTC), 13400-970 Piracicaba, SP, BrazilBiotechnology Division, Sugarcane Technology Center (CTC), 13400-970 Piracicaba, SP, BrazilInstitute of Molecular Biosciences, Frankfurt University, 60438 Frankfurt am Main, GermanyDepartment of Genetics, Federal University of Pernambuco (UFPE), 50670-901 Recife, PE, BrazilDepartment of Genetics, Federal University of Pernambuco (UFPE), 50670-901 Recife, PE, BrazilThe present work is a pioneer study specifically addressing the aquaporin transcripts in sugarcane transcriptomes. Representatives of the four aquaporin subfamilies (PIP, TIP, SIP, and NIP), already described for higher plants, were identified. Forty-two distinct aquaporin isoforms were expressed in four HT-SuperSAGE libraries from sugarcane roots of drought-tolerant and -sensitive genotypes, respectively. At least 10 different potential aquaporin isoform targets and their respective unitags were considered to be promising for future studies and especially for the development of molecular markers for plant breeding. From those 10 isoforms, four (SoPIP2-4, SoPIP2-6, OsPIP2-4, and SsPIP1-1) showed distinct responses towards drought, with divergent expressions between the bulks from tolerant and sensitive genotypes, when they were compared under normal and stress conditions. Two targets (SsPIP1-1 and SoPIP1-3/PIP1-4) were selected for validation via RT-qPCR and their expression patterns as detected by HT-SuperSAGE were confirmed. The employed validation strategy revealed that different genotypes share the same tolerant or sensitive phenotype, respectively, but may use different routes for stress acclimation, indicating the aquaporin transcription in sugarcane to be potentially genotype-specific.http://dx.doi.org/10.1155/2013/763945 |
| spellingShingle | Manassés Daniel da Silva Roberta Lane de Oliveira Silva José Ribamar Costa Ferreira Neto Ana Carolina Ribeiro Guimarães Daniela Truffi Veiga Sabrina Moutinho Chabregas William Lee Burnquist Günter Kahl Ana Maria Benko-Iseppon Ederson Akio Kido Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit Journal of Nucleic Acids |
| title | Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit |
| title_full | Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit |
| title_fullStr | Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit |
| title_full_unstemmed | Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit |
| title_short | Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit |
| title_sort | expression analysis of sugarcane aquaporin genes under water deficit |
| url | http://dx.doi.org/10.1155/2013/763945 |
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