Seminal plasma protein profiles based on molecular weight as biomarkers of sperm fertility in horned and polled Bali bulls
Background and Aim: Seminal plasma proteins (SPPs) significantly influence sperm quality, playing a critical role in fertility. This study aims to investigate the molecular weight (MW) profiles of SPPs in horned and polled Bali bulls and their correlation with sperm quality parameters. Materials...
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Veterinary World
2025-01-01
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| Series: | Veterinary World |
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| Online Access: | https://www.veterinaryworld.org/Vol.18/January-2025/14.pdf |
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| author | Rasyidah Mappanganro Herry Sonjaya Sudirman Baco Hasbi Hasbi Sri Gustina |
| author_facet | Rasyidah Mappanganro Herry Sonjaya Sudirman Baco Hasbi Hasbi Sri Gustina |
| author_sort | Rasyidah Mappanganro |
| collection | DOAJ |
| description | Background and Aim: Seminal plasma proteins (SPPs) significantly influence sperm quality, playing a critical role in fertility. This study aims to investigate the molecular weight (MW) profiles of SPPs in horned and polled Bali bulls and their correlation with sperm quality parameters.
Materials and Methods: Semen samples were collected from six Bali bulls (3 horned, 3 polled). Sperm quality was evaluated based on motility, viability, abnormalities, intact membranes, and acrosomes. SPPs were extracted and analyzed using one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine protein MWs. Pearson’s correlation was used to analyze relationships between MW profiles and sperm quality metrics.
Results: SPPs were identified across a MW range of 15–165 kDa, with specific proteins showing strong correlations with sperm quality. Proteins at 50 and 46 kDa positively correlated with motility (r = –0.96), viability (r = –0.99), and intact membranes (r = –0.86). Conversely, proteins at 40 kDa negatively correlated with these parameters. A 25 kDa protein displayed a positive correlation with intact acrosomes (r = –0.93) and a negative correlation with abnormalities (r = –0.99). Differences in sperm quality metrics between horned and polled bulls were observed, with polled bulls exhibiting fewer abnormalities.
Conclusion: This study highlights the potential of SPP MW profiles as biomarkers of sperm quality in Bali bulls. Proteins at 50, 46, and 25 kDa are promising markers for sperm motility, viability, and intact acrosomes, respectively. These findings could inform bull selection and reproductive management strategies. Further research is recommended to validate these biomarkers using advanced proteomic approaches. |
| format | Article |
| id | doaj-art-44818888cfa04657aff439881666df24 |
| institution | Kabale University |
| issn | 0972-8988 2231-0916 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj-art-44818888cfa04657aff439881666df242025-01-26T04:34:57ZengVeterinary WorldVeterinary World0972-89882231-09162025-01-0118112213210.14202/vetworld.2025.122-132Seminal plasma protein profiles based on molecular weight as biomarkers of sperm fertility in horned and polled Bali bullsRasyidah Mappanganro0https://orcid.org/0000-0002-0775-2438Herry Sonjaya1https://orcid.org/0000-0002-8545-4110Sudirman Baco2https://orcid.org/0000-0002-1819-3096Hasbi Hasbi3https://orcid.org/0000-0002-6069-2171Sri Gustina4https://orcid.org/0000-0003-1466-4740Department of Animal Science, Faculty of Science and Technology, Universitas Islam Negeri Alauddin Makassar, Indonesia; Animal Science Study Program, Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia.Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia.Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia.Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia.Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia.Background and Aim: Seminal plasma proteins (SPPs) significantly influence sperm quality, playing a critical role in fertility. This study aims to investigate the molecular weight (MW) profiles of SPPs in horned and polled Bali bulls and their correlation with sperm quality parameters. Materials and Methods: Semen samples were collected from six Bali bulls (3 horned, 3 polled). Sperm quality was evaluated based on motility, viability, abnormalities, intact membranes, and acrosomes. SPPs were extracted and analyzed using one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine protein MWs. Pearson’s correlation was used to analyze relationships between MW profiles and sperm quality metrics. Results: SPPs were identified across a MW range of 15–165 kDa, with specific proteins showing strong correlations with sperm quality. Proteins at 50 and 46 kDa positively correlated with motility (r = –0.96), viability (r = –0.99), and intact membranes (r = –0.86). Conversely, proteins at 40 kDa negatively correlated with these parameters. A 25 kDa protein displayed a positive correlation with intact acrosomes (r = –0.93) and a negative correlation with abnormalities (r = –0.99). Differences in sperm quality metrics between horned and polled bulls were observed, with polled bulls exhibiting fewer abnormalities. Conclusion: This study highlights the potential of SPP MW profiles as biomarkers of sperm quality in Bali bulls. Proteins at 50, 46, and 25 kDa are promising markers for sperm motility, viability, and intact acrosomes, respectively. These findings could inform bull selection and reproductive management strategies. Further research is recommended to validate these biomarkers using advanced proteomic approaches.https://www.veterinaryworld.org/Vol.18/January-2025/14.pdfbali bullsmolecular weightseminal plasma proteinssodium dodecyl sulfate-polyacrylamide gel electrophoresissperm fertility |
| spellingShingle | Rasyidah Mappanganro Herry Sonjaya Sudirman Baco Hasbi Hasbi Sri Gustina Seminal plasma protein profiles based on molecular weight as biomarkers of sperm fertility in horned and polled Bali bulls Veterinary World bali bulls molecular weight seminal plasma proteins sodium dodecyl sulfate-polyacrylamide gel electrophoresis sperm fertility |
| title | Seminal plasma protein profiles based on molecular weight as biomarkers of sperm fertility in horned and polled Bali bulls |
| title_full | Seminal plasma protein profiles based on molecular weight as biomarkers of sperm fertility in horned and polled Bali bulls |
| title_fullStr | Seminal plasma protein profiles based on molecular weight as biomarkers of sperm fertility in horned and polled Bali bulls |
| title_full_unstemmed | Seminal plasma protein profiles based on molecular weight as biomarkers of sperm fertility in horned and polled Bali bulls |
| title_short | Seminal plasma protein profiles based on molecular weight as biomarkers of sperm fertility in horned and polled Bali bulls |
| title_sort | seminal plasma protein profiles based on molecular weight as biomarkers of sperm fertility in horned and polled bali bulls |
| topic | bali bulls molecular weight seminal plasma proteins sodium dodecyl sulfate-polyacrylamide gel electrophoresis sperm fertility |
| url | https://www.veterinaryworld.org/Vol.18/January-2025/14.pdf |
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