Profile of Secondary Metabolite Compounds in Soybean (Glycine max L.) Leaves under Different Shading Treatments

This study aims to assess the impact of shading treatment variations on soybean leaf metabolites. The research was conducted as follows: (i) planting several soybean varieties under different shading intensities; (ii) extracting metabolic substances from soybean leaves through maceration; (iii)...

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Main Authors: Deddy Wahyudin Purba, Suswati Suswati
Format: Article
Language:English
Published: Society for Innovative Agriculture 2025-01-01
Series:Journal of Global Innovations in Agricultural Sciences
Online Access:https://jgiass.com/pdf-reader.php?file=Profile-of-Secondary-Metabolite-Compounds-in-Soybean-(Glycine-max-L.)-Leaves-under-Different-Shading-Treatments.pdf&path=issue_papers
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author Deddy Wahyudin Purba
Suswati Suswati
author_facet Deddy Wahyudin Purba
Suswati Suswati
author_sort Deddy Wahyudin Purba
collection DOAJ
description This study aims to assess the impact of shading treatment variations on soybean leaf metabolites. The research was conducted as follows: (i) planting several soybean varieties under different shading intensities; (ii) extracting metabolic substances from soybean leaves through maceration; (iii) identifying the extracted substances using High-Performance Liquid Chromatography (HPLC); and (iv) analyzing changes in primary metabolic substances. This study applied different shading treatments to various local soybean varieties: Anjasmoro, Mutiara 1, Denasa 1, Denasa 2, Dena 1, and Dena 2. The results indicate that among the different varieties and shading treatments, the highest isoflavone content was observed in Dena 2 with shading treatment on producing plants (1.820 g), followed by Anjasmoro with shading treatment on non-producing plants (1.788 g), and Dena 2 with no shading treatment (1.780 g). In addition, the statistical analysis revealed that shading treatments show significant differences only in extract mass content treated using fermentation on day 1 (p-value = 0.011) and day 3 (p-value = 0.039). These findings provide valuable insights for the scientific cultivation, management, and large-scale breeding of soybeans, specifically offering guidance on optimal shading levels for improved growth and metabolite production. Keywords: Photosynthetic efficiency, isoflavone accumulation, crop management, metabolic profiling, shading effects.
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institution Kabale University
issn 2788-4538
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publishDate 2025-01-01
publisher Society for Innovative Agriculture
record_format Article
series Journal of Global Innovations in Agricultural Sciences
spelling doaj-art-446aa477906b4369a733cba5fc9956d42025-01-26T04:45:33ZengSociety for Innovative AgricultureJournal of Global Innovations in Agricultural Sciences2788-45382788-45462025-01-0119320210.22194/JGIAS/25.1399Profile of Secondary Metabolite Compounds in Soybean (Glycine max L.) Leaves under Different Shading TreatmentsDeddy Wahyudin PurbaSuswati Suswati This study aims to assess the impact of shading treatment variations on soybean leaf metabolites. The research was conducted as follows: (i) planting several soybean varieties under different shading intensities; (ii) extracting metabolic substances from soybean leaves through maceration; (iii) identifying the extracted substances using High-Performance Liquid Chromatography (HPLC); and (iv) analyzing changes in primary metabolic substances. This study applied different shading treatments to various local soybean varieties: Anjasmoro, Mutiara 1, Denasa 1, Denasa 2, Dena 1, and Dena 2. The results indicate that among the different varieties and shading treatments, the highest isoflavone content was observed in Dena 2 with shading treatment on producing plants (1.820 g), followed by Anjasmoro with shading treatment on non-producing plants (1.788 g), and Dena 2 with no shading treatment (1.780 g). In addition, the statistical analysis revealed that shading treatments show significant differences only in extract mass content treated using fermentation on day 1 (p-value = 0.011) and day 3 (p-value = 0.039). These findings provide valuable insights for the scientific cultivation, management, and large-scale breeding of soybeans, specifically offering guidance on optimal shading levels for improved growth and metabolite production. Keywords: Photosynthetic efficiency, isoflavone accumulation, crop management, metabolic profiling, shading effects. https://jgiass.com/pdf-reader.php?file=Profile-of-Secondary-Metabolite-Compounds-in-Soybean-(Glycine-max-L.)-Leaves-under-Different-Shading-Treatments.pdf&path=issue_papers
spellingShingle Deddy Wahyudin Purba
Suswati Suswati
Profile of Secondary Metabolite Compounds in Soybean (Glycine max L.) Leaves under Different Shading Treatments
Journal of Global Innovations in Agricultural Sciences
title Profile of Secondary Metabolite Compounds in Soybean (Glycine max L.) Leaves under Different Shading Treatments
title_full Profile of Secondary Metabolite Compounds in Soybean (Glycine max L.) Leaves under Different Shading Treatments
title_fullStr Profile of Secondary Metabolite Compounds in Soybean (Glycine max L.) Leaves under Different Shading Treatments
title_full_unstemmed Profile of Secondary Metabolite Compounds in Soybean (Glycine max L.) Leaves under Different Shading Treatments
title_short Profile of Secondary Metabolite Compounds in Soybean (Glycine max L.) Leaves under Different Shading Treatments
title_sort profile of secondary metabolite compounds in soybean glycine max l leaves under different shading treatments
url https://jgiass.com/pdf-reader.php?file=Profile-of-Secondary-Metabolite-Compounds-in-Soybean-(Glycine-max-L.)-Leaves-under-Different-Shading-Treatments.pdf&path=issue_papers
work_keys_str_mv AT deddywahyudinpurba profileofsecondarymetabolitecompoundsinsoybeanglycinemaxlleavesunderdifferentshadingtreatments
AT suswatisuswati profileofsecondarymetabolitecompoundsinsoybeanglycinemaxlleavesunderdifferentshadingtreatments