Separating and Characterizing Functional Nitrogen Degraders via Magnetic Nanoparticle-Mediated Isolation
Magnetic nanoparticle-mediated isolation (MMI) is a new method for isolating active functional microbes from complex microorganisms without substrate labeling. In this study, the composition and properties of magnetic nanoparticles (MNPs) were characterized by a number of techniques, indicating that...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/1841364 |
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| _version_ | 1832560660195573760 |
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| author | Yujiao Sun Meng Yin Danyang Zheng Lei Wang Xiaohui Zhao Jie Li |
| author_facet | Yujiao Sun Meng Yin Danyang Zheng Lei Wang Xiaohui Zhao Jie Li |
| author_sort | Yujiao Sun |
| collection | DOAJ |
| description | Magnetic nanoparticle-mediated isolation (MMI) is a new method for isolating active functional microbes from complex microorganisms without substrate labeling. In this study, the composition and properties of magnetic nanoparticles (MNPs) were characterized by a number of techniques, indicating that MNPs have characteristics such as microinterfaces and can be efficiently fixed on the surface of microbial cells. It also introduced the MMI technology in activated sludge after stable long-term treatment. With further addition of promotor carbon sources, the enrichment of the functional nitrogen degraders in MMI was significantly higher than in samples without MNPs, showing the advantages of MMI in identifying the active degraders. Redundancy analysis (RDA) also showed that the functional nitrogen degraders such as Comamonadaceae_unclassified and Thiobacillus absolutely dominated in situ ammonia degradation, and the change in dominant genera had the same trend as the degradation rate of ammonia nitrogen. In the magnetically functionalized system, the separated functional nitrogen degraders significantly improved ammonia nitrogen degradation efficiency, making it basically stable at more than 80%, up to 91.6%. These results prove that the complex flora created after the addition of MNPs is more adaptable to newly introduced pollutants, and MMI is a powerful tool for studying pollutant-degrading microorganisms under in situ conditions. |
| format | Article |
| id | doaj-art-44626c6c045e4645a866aa4c55259038 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-44626c6c045e4645a866aa4c552590382025-02-03T01:26:57ZengWileyJournal of Chemistry2090-90632090-90712020-01-01202010.1155/2020/18413641841364Separating and Characterizing Functional Nitrogen Degraders via Magnetic Nanoparticle-Mediated IsolationYujiao Sun0Meng Yin1Danyang Zheng2Lei Wang3Xiaohui Zhao4Jie Li5College of Water Science, Beijing Normal University, Beijing 100875, ChinaCollege of Water Science, Beijing Normal University, Beijing 100875, ChinaCollege of Water Science, Beijing Normal University, Beijing 100875, ChinaCollege of Water Science, Beijing Normal University, Beijing 100875, ChinaCollege of Water Science, Beijing Normal University, Beijing 100875, ChinaCollege of Water Science, Beijing Normal University, Beijing 100875, ChinaMagnetic nanoparticle-mediated isolation (MMI) is a new method for isolating active functional microbes from complex microorganisms without substrate labeling. In this study, the composition and properties of magnetic nanoparticles (MNPs) were characterized by a number of techniques, indicating that MNPs have characteristics such as microinterfaces and can be efficiently fixed on the surface of microbial cells. It also introduced the MMI technology in activated sludge after stable long-term treatment. With further addition of promotor carbon sources, the enrichment of the functional nitrogen degraders in MMI was significantly higher than in samples without MNPs, showing the advantages of MMI in identifying the active degraders. Redundancy analysis (RDA) also showed that the functional nitrogen degraders such as Comamonadaceae_unclassified and Thiobacillus absolutely dominated in situ ammonia degradation, and the change in dominant genera had the same trend as the degradation rate of ammonia nitrogen. In the magnetically functionalized system, the separated functional nitrogen degraders significantly improved ammonia nitrogen degradation efficiency, making it basically stable at more than 80%, up to 91.6%. These results prove that the complex flora created after the addition of MNPs is more adaptable to newly introduced pollutants, and MMI is a powerful tool for studying pollutant-degrading microorganisms under in situ conditions.http://dx.doi.org/10.1155/2020/1841364 |
| spellingShingle | Yujiao Sun Meng Yin Danyang Zheng Lei Wang Xiaohui Zhao Jie Li Separating and Characterizing Functional Nitrogen Degraders via Magnetic Nanoparticle-Mediated Isolation Journal of Chemistry |
| title | Separating and Characterizing Functional Nitrogen Degraders via Magnetic Nanoparticle-Mediated Isolation |
| title_full | Separating and Characterizing Functional Nitrogen Degraders via Magnetic Nanoparticle-Mediated Isolation |
| title_fullStr | Separating and Characterizing Functional Nitrogen Degraders via Magnetic Nanoparticle-Mediated Isolation |
| title_full_unstemmed | Separating and Characterizing Functional Nitrogen Degraders via Magnetic Nanoparticle-Mediated Isolation |
| title_short | Separating and Characterizing Functional Nitrogen Degraders via Magnetic Nanoparticle-Mediated Isolation |
| title_sort | separating and characterizing functional nitrogen degraders via magnetic nanoparticle mediated isolation |
| url | http://dx.doi.org/10.1155/2020/1841364 |
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