Human Mesenchymal Stromal Cells Transplantation May Enhance or Inhibit 4T1 Murine Breast Adenocarcinoma through Different Approaches
The use of Mesenchymal Stromal Cells (MSCs) aiming to treat cancer has shown very contradictory results. In an attempt to clarify the contradictory results reported in the literature and the possible role of human fallopian tube Mesenchymal Stromal Cells (htMSCs) against breast cancer, the aim of th...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2015/796215 |
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| author | T. Jazedje A. L. Ribeiro M. Pellati H. M. de Siqueira Bueno G. Nagata M. Trierveiler E. G. Rodrigues M. Zatz |
| author_facet | T. Jazedje A. L. Ribeiro M. Pellati H. M. de Siqueira Bueno G. Nagata M. Trierveiler E. G. Rodrigues M. Zatz |
| author_sort | T. Jazedje |
| collection | DOAJ |
| description | The use of Mesenchymal Stromal Cells (MSCs) aiming to treat cancer has shown very contradictory results. In an attempt to clarify the contradictory results reported in the literature and the possible role of human fallopian tube Mesenchymal Stromal Cells (htMSCs) against breast cancer, the aim of this study was to evaluate the clinical effect of htMSCs in murine mammary adenocarcinoma using two different approaches: (1) coinjections of htMSCs and 4T1 murine tumor cell lineage and (2) injections of htMSCs in mice at the initial stage of mammary adenocarcinoma development. Coinjected animals had a more severe course of the disease and a reduced survival, while tumor-bearing animals treated with 2 intraperitoneal injections of 106 htMSCs showed significantly reduced tumor growth and increased lifespan as compared with control animals. Coculture of htMSCs and 4T1 tumor cells revealed an increase in IL-8 and MCP-1 and decreased VEGF production. For the first time, we show that MSCs isolated from a single source and donor when injected in the same animal model and tumor can lead to opposite results depending on the experimental protocol. Also, our results demonstrated that htMSCs can have an inhibitory effect on the development of murine mammary adenocarcinoma. |
| format | Article |
| id | doaj-art-4448f1ca8ad344248990a5a49061786a |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-4448f1ca8ad344248990a5a49061786a2025-02-03T06:11:27ZengWileyStem Cells International1687-966X1687-96782015-01-01201510.1155/2015/796215796215Human Mesenchymal Stromal Cells Transplantation May Enhance or Inhibit 4T1 Murine Breast Adenocarcinoma through Different ApproachesT. Jazedje0A. L. Ribeiro1M. Pellati2H. M. de Siqueira Bueno3G. Nagata4M. Trierveiler5E. G. Rodrigues6M. Zatz7Human Genome and Stem-Cell Center (HUG-CELL), Institute of Bioscience, University of São Paulo, 05508-090 São Paulo, SP, BrazilLaboratory of Cancer Immunobiology, Department of Microbiology, Immunology and Parasitology, Paulista School of Medicine, Federal University of São Paulo (EPM-UNIFESP), 04023-062 São Paulo, SP, BrazilHuman Genome and Stem-Cell Center (HUG-CELL), Institute of Bioscience, University of São Paulo, 05508-090 São Paulo, SP, BrazilHuman Genome and Stem-Cell Center (HUG-CELL), Institute of Bioscience, University of São Paulo, 05508-090 São Paulo, SP, BrazilStomatology Department, Faculty of Dentistry, University of São Paulo, 05508-000 São Paulo, SP, BrazilStomatology Department, Faculty of Dentistry, University of São Paulo, 05508-000 São Paulo, SP, BrazilLaboratory of Cancer Immunobiology, Department of Microbiology, Immunology and Parasitology, Paulista School of Medicine, Federal University of São Paulo (EPM-UNIFESP), 04023-062 São Paulo, SP, BrazilHuman Genome and Stem-Cell Center (HUG-CELL), Institute of Bioscience, University of São Paulo, 05508-090 São Paulo, SP, BrazilThe use of Mesenchymal Stromal Cells (MSCs) aiming to treat cancer has shown very contradictory results. In an attempt to clarify the contradictory results reported in the literature and the possible role of human fallopian tube Mesenchymal Stromal Cells (htMSCs) against breast cancer, the aim of this study was to evaluate the clinical effect of htMSCs in murine mammary adenocarcinoma using two different approaches: (1) coinjections of htMSCs and 4T1 murine tumor cell lineage and (2) injections of htMSCs in mice at the initial stage of mammary adenocarcinoma development. Coinjected animals had a more severe course of the disease and a reduced survival, while tumor-bearing animals treated with 2 intraperitoneal injections of 106 htMSCs showed significantly reduced tumor growth and increased lifespan as compared with control animals. Coculture of htMSCs and 4T1 tumor cells revealed an increase in IL-8 and MCP-1 and decreased VEGF production. For the first time, we show that MSCs isolated from a single source and donor when injected in the same animal model and tumor can lead to opposite results depending on the experimental protocol. Also, our results demonstrated that htMSCs can have an inhibitory effect on the development of murine mammary adenocarcinoma.http://dx.doi.org/10.1155/2015/796215 |
| spellingShingle | T. Jazedje A. L. Ribeiro M. Pellati H. M. de Siqueira Bueno G. Nagata M. Trierveiler E. G. Rodrigues M. Zatz Human Mesenchymal Stromal Cells Transplantation May Enhance or Inhibit 4T1 Murine Breast Adenocarcinoma through Different Approaches Stem Cells International |
| title | Human Mesenchymal Stromal Cells Transplantation May Enhance or Inhibit 4T1 Murine Breast Adenocarcinoma through Different Approaches |
| title_full | Human Mesenchymal Stromal Cells Transplantation May Enhance or Inhibit 4T1 Murine Breast Adenocarcinoma through Different Approaches |
| title_fullStr | Human Mesenchymal Stromal Cells Transplantation May Enhance or Inhibit 4T1 Murine Breast Adenocarcinoma through Different Approaches |
| title_full_unstemmed | Human Mesenchymal Stromal Cells Transplantation May Enhance or Inhibit 4T1 Murine Breast Adenocarcinoma through Different Approaches |
| title_short | Human Mesenchymal Stromal Cells Transplantation May Enhance or Inhibit 4T1 Murine Breast Adenocarcinoma through Different Approaches |
| title_sort | human mesenchymal stromal cells transplantation may enhance or inhibit 4t1 murine breast adenocarcinoma through different approaches |
| url | http://dx.doi.org/10.1155/2015/796215 |
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