Genomic Landscape Reveals Chromosomally-Mediated Antimicrobial Resistome and Virulome of a High-Risk International Clone II Acinetobacter baumannii AB073 from Thailand
This study utilized integrative bioinformatics’ tools together with phenotypic assays to understand the whole-genome features of a carbapenem-resistant international clone II Acinetobacter baumannii AB073. Overall, we found the isolate to be resistant to seven antibiotic classes, penicillins, β-lact...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Global Health, Epidemiology and Genomics |
| Online Access: | http://dx.doi.org/10.1155/2024/8872463 |
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| author | Rattiya Cheewapat Jadsadaporn Redkimned Sirikran Lekuthai Thawatchai Kitti Kannipa Tasanapak Aunchalee Thanwisai Sutthirat Sitthisak Thanet Sornda Hathaichanok Impheng Sudarat Onsurathum Udomluk Leungtongkam Supaporn Lamlertthon Siriwat Kucharoenphaibul Jintana Wongwigkarn Pantira Singkum Pattrarat Chanchaithong Rapee Thummeepak |
| author_facet | Rattiya Cheewapat Jadsadaporn Redkimned Sirikran Lekuthai Thawatchai Kitti Kannipa Tasanapak Aunchalee Thanwisai Sutthirat Sitthisak Thanet Sornda Hathaichanok Impheng Sudarat Onsurathum Udomluk Leungtongkam Supaporn Lamlertthon Siriwat Kucharoenphaibul Jintana Wongwigkarn Pantira Singkum Pattrarat Chanchaithong Rapee Thummeepak |
| author_sort | Rattiya Cheewapat |
| collection | DOAJ |
| description | This study utilized integrative bioinformatics’ tools together with phenotypic assays to understand the whole-genome features of a carbapenem-resistant international clone II Acinetobacter baumannii AB073. Overall, we found the isolate to be resistant to seven antibiotic classes, penicillins, β-lactam/β-lactamase inhibitor combinations, cephalosporins, carbapenems, aminoglycosides, fluoroquinolones, and folate pathway antagonists. These resistance phenotypes are related to various chromosomal-located antibiotic resistance determinants involved in different mechanisms such as reduced permeability, antibiotic target protection, antibiotic target alteration, antibiotic inactivation, and antibiotic efflux. IC2 A. baumannii AB073 could not transfer antibiotic resistance by conjugation experiments. Likewise, mobilome analysis found that AB073 did not carry genetic determinants involving horizontal gene transfer. Moreover, this isolate also carried multiple genes associated with the ability of iron uptake, biofilm formation, immune invasion, virulence regulations, and serum resistance. In addition, the genomic epidemiological study showed that AB073-like strains were successful pathogens widespread in various geographic locations and clinical sources. In conclusion, the comprehensive analysis demonstrated that AB073 contained multiple genomic determinants which were important characteristics to classify this isolate as a successful international clone II obtained from Thailand. |
| format | Article |
| id | doaj-art-442ceb2062234dadab9608e79d767804 |
| institution | Kabale University |
| issn | 2054-4200 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Global Health, Epidemiology and Genomics |
| spelling | doaj-art-442ceb2062234dadab9608e79d7678042025-02-03T05:56:54ZengWileyGlobal Health, Epidemiology and Genomics2054-42002024-01-01202410.1155/2024/8872463Genomic Landscape Reveals Chromosomally-Mediated Antimicrobial Resistome and Virulome of a High-Risk International Clone II Acinetobacter baumannii AB073 from ThailandRattiya Cheewapat0Jadsadaporn Redkimned1Sirikran Lekuthai2Thawatchai Kitti3Kannipa Tasanapak4Aunchalee Thanwisai5Sutthirat Sitthisak6Thanet Sornda7Hathaichanok Impheng8Sudarat Onsurathum9Udomluk Leungtongkam10Supaporn Lamlertthon11Siriwat Kucharoenphaibul12Jintana Wongwigkarn13Pantira Singkum14Pattrarat Chanchaithong15Rapee Thummeepak16Department of Microbiology and ParasitologyDepartment of Microbiology and ParasitologyDepartment of Microbiology and ParasitologyDepartment of Oriental MedicineDepartment of Microbiology and ParasitologyDepartment of Microbiology and ParasitologyDepartment of Microbiology and ParasitologyDepartment of BiochemistryDepartment of PhysiologyDepartment of Microbiology and ParasitologyDepartment of Microbiology and ParasitologyDepartment of Microbiology and ParasitologyDepartment of Microbiology and ParasitologyDepartment of Microbiology and ParasitologyDepartment of Microbiology and ImmunologyDepartment of Veterinary MicrobiologyDepartment of Microbiology and ParasitologyThis study utilized integrative bioinformatics’ tools together with phenotypic assays to understand the whole-genome features of a carbapenem-resistant international clone II Acinetobacter baumannii AB073. Overall, we found the isolate to be resistant to seven antibiotic classes, penicillins, β-lactam/β-lactamase inhibitor combinations, cephalosporins, carbapenems, aminoglycosides, fluoroquinolones, and folate pathway antagonists. These resistance phenotypes are related to various chromosomal-located antibiotic resistance determinants involved in different mechanisms such as reduced permeability, antibiotic target protection, antibiotic target alteration, antibiotic inactivation, and antibiotic efflux. IC2 A. baumannii AB073 could not transfer antibiotic resistance by conjugation experiments. Likewise, mobilome analysis found that AB073 did not carry genetic determinants involving horizontal gene transfer. Moreover, this isolate also carried multiple genes associated with the ability of iron uptake, biofilm formation, immune invasion, virulence regulations, and serum resistance. In addition, the genomic epidemiological study showed that AB073-like strains were successful pathogens widespread in various geographic locations and clinical sources. In conclusion, the comprehensive analysis demonstrated that AB073 contained multiple genomic determinants which were important characteristics to classify this isolate as a successful international clone II obtained from Thailand.http://dx.doi.org/10.1155/2024/8872463 |
| spellingShingle | Rattiya Cheewapat Jadsadaporn Redkimned Sirikran Lekuthai Thawatchai Kitti Kannipa Tasanapak Aunchalee Thanwisai Sutthirat Sitthisak Thanet Sornda Hathaichanok Impheng Sudarat Onsurathum Udomluk Leungtongkam Supaporn Lamlertthon Siriwat Kucharoenphaibul Jintana Wongwigkarn Pantira Singkum Pattrarat Chanchaithong Rapee Thummeepak Genomic Landscape Reveals Chromosomally-Mediated Antimicrobial Resistome and Virulome of a High-Risk International Clone II Acinetobacter baumannii AB073 from Thailand Global Health, Epidemiology and Genomics |
| title | Genomic Landscape Reveals Chromosomally-Mediated Antimicrobial Resistome and Virulome of a High-Risk International Clone II Acinetobacter baumannii AB073 from Thailand |
| title_full | Genomic Landscape Reveals Chromosomally-Mediated Antimicrobial Resistome and Virulome of a High-Risk International Clone II Acinetobacter baumannii AB073 from Thailand |
| title_fullStr | Genomic Landscape Reveals Chromosomally-Mediated Antimicrobial Resistome and Virulome of a High-Risk International Clone II Acinetobacter baumannii AB073 from Thailand |
| title_full_unstemmed | Genomic Landscape Reveals Chromosomally-Mediated Antimicrobial Resistome and Virulome of a High-Risk International Clone II Acinetobacter baumannii AB073 from Thailand |
| title_short | Genomic Landscape Reveals Chromosomally-Mediated Antimicrobial Resistome and Virulome of a High-Risk International Clone II Acinetobacter baumannii AB073 from Thailand |
| title_sort | genomic landscape reveals chromosomally mediated antimicrobial resistome and virulome of a high risk international clone ii acinetobacter baumannii ab073 from thailand |
| url | http://dx.doi.org/10.1155/2024/8872463 |
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