Early Growth Response Genes Signaling Supports Strong Paracrine Capability of Mesenchymal Stem Cells
MSCs provide a promising method for cell therapy through their wound healing and tissue regenerative properties. Originally, MSCs' role in wound healing was thought to be tied to their multipotency, but it is now accepted that MSCs mediate the healing process through their strong paracrine capa...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2012/428403 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832554903723048960 |
|---|---|
| author | Kenichi Tamama Dominique J. Barbeau |
| author_facet | Kenichi Tamama Dominique J. Barbeau |
| author_sort | Kenichi Tamama |
| collection | DOAJ |
| description | MSCs provide a promising method for cell therapy through their wound healing and tissue regenerative properties. Originally, MSCs' role in wound healing was thought to be tied to their multipotency, but it is now accepted that MSCs mediate the healing process through their strong paracrine capability. EGF was shown to facilitate in vitro expansion of MSCs without altering multipotency. Our previous data suggest that the molecular machinery underlying MSCs' strong paracrine capability lies downstream of EGFR signaling, and we focus on transcription factors EGR1 and EGR2. Evidence suggests that EGR1 regulates angiogenic and fibrogenic factor production in MSCs, and an EGFR-EGR1-EGFR ligands autocrine loop is one of the underlying mechanisms supporting their strong paracrine machinery through EGR1. EGR2 appears to regulate the expression of immunomodulatory molecules. Chronic nonhealing wounds are ischemic, inflammatory, and often fibrotic, and the hypoxic micro-environment of these wounds may compromise MSCs' wound healing properties in vivo by upregulating the EGR1's fibrogenic effects and downregulating the EGR2's immuno-modulatory effects. Thus, these transcription factors can be potential targets in the optimization of cell-based therapies. Further study in vitro is required to understand MSCs' paracrine machinery and to optimize it as a tool for effective cell-based therapies. |
| format | Article |
| id | doaj-art-442ce3a8c2184b279a1f50d724d1f008 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-442ce3a8c2184b279a1f50d724d1f0082025-02-03T05:50:14ZengWileyStem Cells International1687-966X1687-96782012-01-01201210.1155/2012/428403428403Early Growth Response Genes Signaling Supports Strong Paracrine Capability of Mesenchymal Stem CellsKenichi Tamama0Dominique J. Barbeau1Department of Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USADepartment of Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USAMSCs provide a promising method for cell therapy through their wound healing and tissue regenerative properties. Originally, MSCs' role in wound healing was thought to be tied to their multipotency, but it is now accepted that MSCs mediate the healing process through their strong paracrine capability. EGF was shown to facilitate in vitro expansion of MSCs without altering multipotency. Our previous data suggest that the molecular machinery underlying MSCs' strong paracrine capability lies downstream of EGFR signaling, and we focus on transcription factors EGR1 and EGR2. Evidence suggests that EGR1 regulates angiogenic and fibrogenic factor production in MSCs, and an EGFR-EGR1-EGFR ligands autocrine loop is one of the underlying mechanisms supporting their strong paracrine machinery through EGR1. EGR2 appears to regulate the expression of immunomodulatory molecules. Chronic nonhealing wounds are ischemic, inflammatory, and often fibrotic, and the hypoxic micro-environment of these wounds may compromise MSCs' wound healing properties in vivo by upregulating the EGR1's fibrogenic effects and downregulating the EGR2's immuno-modulatory effects. Thus, these transcription factors can be potential targets in the optimization of cell-based therapies. Further study in vitro is required to understand MSCs' paracrine machinery and to optimize it as a tool for effective cell-based therapies.http://dx.doi.org/10.1155/2012/428403 |
| spellingShingle | Kenichi Tamama Dominique J. Barbeau Early Growth Response Genes Signaling Supports Strong Paracrine Capability of Mesenchymal Stem Cells Stem Cells International |
| title | Early Growth Response Genes Signaling Supports Strong Paracrine Capability of Mesenchymal Stem Cells |
| title_full | Early Growth Response Genes Signaling Supports Strong Paracrine Capability of Mesenchymal Stem Cells |
| title_fullStr | Early Growth Response Genes Signaling Supports Strong Paracrine Capability of Mesenchymal Stem Cells |
| title_full_unstemmed | Early Growth Response Genes Signaling Supports Strong Paracrine Capability of Mesenchymal Stem Cells |
| title_short | Early Growth Response Genes Signaling Supports Strong Paracrine Capability of Mesenchymal Stem Cells |
| title_sort | early growth response genes signaling supports strong paracrine capability of mesenchymal stem cells |
| url | http://dx.doi.org/10.1155/2012/428403 |
| work_keys_str_mv | AT kenichitamama earlygrowthresponsegenessignalingsupportsstrongparacrinecapabilityofmesenchymalstemcells AT dominiquejbarbeau earlygrowthresponsegenessignalingsupportsstrongparacrinecapabilityofmesenchymalstemcells |