Hot and cold exposure triggers distinct transcriptional and behavioral responses in laboratory-inbred pond snails
Animals exhibit remarkable behavioral and molecular adaptations to cope with thermal stressors, which are crucial for survival in variable environments that are exacerbated by climate change. Aquatic poikilotherms like our model organism—the pond snail Lymnaea stagnalis—face significant challenges d...
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KeAi Communications Co. Ltd.
2025-01-01
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| Series: | Water Biology and Security |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772735124000878 |
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| author | Veronica Rivi Anuradha Batabyal Cristina Benatti Fabio Tascedda Johanna Maria Catharina Blom Ken Lukowiak |
| author_facet | Veronica Rivi Anuradha Batabyal Cristina Benatti Fabio Tascedda Johanna Maria Catharina Blom Ken Lukowiak |
| author_sort | Veronica Rivi |
| collection | DOAJ |
| description | Animals exhibit remarkable behavioral and molecular adaptations to cope with thermal stressors, which are crucial for survival in variable environments that are exacerbated by climate change. Aquatic poikilotherms like our model organism—the pond snail Lymnaea stagnalis—face significant challenges due to their dependence on external temperatures. Our study provides valuable insights into the different behavioral and molecular responses of lab-inbred snails to cold and heat shock stressors (i.e., 4 °C and 30 °C), particularly in the context of learning and memory formation. We found that while short-term (1 h) cold exposure transiently upregulated the expression levels of HSP70 and HSP40 in the snail's central ring ganglia, prolonged cold exposure (24 h) resulted in a significant downregulation of LymMIPII and an upregulation of LymMIPR. These data suggest, albeit at the transcriptional level, the existence of a negative feedback loop necessary for sustaining cellular functions when metabolic demands might shift towards conserving energy during prolonged cold exposure. At the behavioral level, we found that, compared to heat shock, cold exposure did not result in a Garcia effect (i.e., a “special form” of conditioned taste aversion). The difference in memory outcomes was associated with changes in the expression levels of selected targets involved in neuronal plasticity and the stress response. While both cold and heat shock upregulated the HSP levels in the snail's central ring ganglia, cold exposure did not affect the expression levels of the neuroplasticity genes LymGRIN1 and LymCREB1, contrasting with heat shock's neurogenic effects. Overall, this study provides insights into L. stagnalis's adaptive responses to thermal stressors, emphasizing different molecular strategies for coping with heat versus cold challenges in aquatic environments. These findings contribute to our understanding of thermal biology and stress physiology in aquatic organisms, underscoring the importance of molecular mechanisms in shaping species' resilience in dynamic environments. |
| format | Article |
| id | doaj-art-44226224b3d041d68feef5600182803e |
| institution | Kabale University |
| issn | 2772-7351 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | KeAi Communications Co. Ltd. |
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| series | Water Biology and Security |
| spelling | doaj-art-44226224b3d041d68feef5600182803e2025-01-27T04:22:41ZengKeAi Communications Co. Ltd.Water Biology and Security2772-73512025-01-0141100315Hot and cold exposure triggers distinct transcriptional and behavioral responses in laboratory-inbred pond snailsVeronica Rivi0Anuradha Batabyal1Cristina Benatti2Fabio Tascedda3Johanna Maria Catharina Blom4Ken Lukowiak5Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena, Italy; Corresponding author.Department of Physiology and Pharmacology, Hotchkiss Brain Institute, Cumming School of Medicine, University of Calgary, Alberta, Canada; Department of Physical and Natural Sciences, FLAME University, Pune, IndiaDepartment of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena, Italy; Centre of Neuroscience and Neurotechnology, University of Modena and Reggio Emilia, Modena, ItalyCentre of Neuroscience and Neurotechnology, University of Modena and Reggio Emilia, Modena, Italy; CIB, Consorzio Interuniversitario Biotecnologie, Trieste, Italy; Department of Life Sciences, University of Modena and Reggio Emilia, Modena, ItalyDepartment of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena, Italy; Department of Life Sciences, University of Modena and Reggio Emilia, Modena, ItalyDepartment of Physiology and Pharmacology, Hotchkiss Brain Institute, Cumming School of Medicine, University of Calgary, Alberta, CanadaAnimals exhibit remarkable behavioral and molecular adaptations to cope with thermal stressors, which are crucial for survival in variable environments that are exacerbated by climate change. Aquatic poikilotherms like our model organism—the pond snail Lymnaea stagnalis—face significant challenges due to their dependence on external temperatures. Our study provides valuable insights into the different behavioral and molecular responses of lab-inbred snails to cold and heat shock stressors (i.e., 4 °C and 30 °C), particularly in the context of learning and memory formation. We found that while short-term (1 h) cold exposure transiently upregulated the expression levels of HSP70 and HSP40 in the snail's central ring ganglia, prolonged cold exposure (24 h) resulted in a significant downregulation of LymMIPII and an upregulation of LymMIPR. These data suggest, albeit at the transcriptional level, the existence of a negative feedback loop necessary for sustaining cellular functions when metabolic demands might shift towards conserving energy during prolonged cold exposure. At the behavioral level, we found that, compared to heat shock, cold exposure did not result in a Garcia effect (i.e., a “special form” of conditioned taste aversion). The difference in memory outcomes was associated with changes in the expression levels of selected targets involved in neuronal plasticity and the stress response. While both cold and heat shock upregulated the HSP levels in the snail's central ring ganglia, cold exposure did not affect the expression levels of the neuroplasticity genes LymGRIN1 and LymCREB1, contrasting with heat shock's neurogenic effects. Overall, this study provides insights into L. stagnalis's adaptive responses to thermal stressors, emphasizing different molecular strategies for coping with heat versus cold challenges in aquatic environments. These findings contribute to our understanding of thermal biology and stress physiology in aquatic organisms, underscoring the importance of molecular mechanisms in shaping species' resilience in dynamic environments.http://www.sciencedirect.com/science/article/pii/S2772735124000878Temperature sensitivityLymnaea stagnalisGarcia effectHSPsCREBEnergy |
| spellingShingle | Veronica Rivi Anuradha Batabyal Cristina Benatti Fabio Tascedda Johanna Maria Catharina Blom Ken Lukowiak Hot and cold exposure triggers distinct transcriptional and behavioral responses in laboratory-inbred pond snails Water Biology and Security Temperature sensitivity Lymnaea stagnalis Garcia effect HSPs CREB Energy |
| title | Hot and cold exposure triggers distinct transcriptional and behavioral responses in laboratory-inbred pond snails |
| title_full | Hot and cold exposure triggers distinct transcriptional and behavioral responses in laboratory-inbred pond snails |
| title_fullStr | Hot and cold exposure triggers distinct transcriptional and behavioral responses in laboratory-inbred pond snails |
| title_full_unstemmed | Hot and cold exposure triggers distinct transcriptional and behavioral responses in laboratory-inbred pond snails |
| title_short | Hot and cold exposure triggers distinct transcriptional and behavioral responses in laboratory-inbred pond snails |
| title_sort | hot and cold exposure triggers distinct transcriptional and behavioral responses in laboratory inbred pond snails |
| topic | Temperature sensitivity Lymnaea stagnalis Garcia effect HSPs CREB Energy |
| url | http://www.sciencedirect.com/science/article/pii/S2772735124000878 |
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