Effects of combined inoculation of arbuscular mycorrhizal fungi and plant growth-promoting rhizosphere bacteria on seedling growth and rhizosphere microecology
The effects of rhizosphere microorganisms on plant growth and the associated mechanisms are a focus of current research, but the effects of exogenous combined inoculation with arbuscular mycorrhizal fungi (AMF) and plant growth-promoting rhizobacteria (PGPR) on seedling growth and the associated rhi...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1475485/full |
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| author | Wanli Zeng Dan Xiang Xuemei Li Qian Gao Yudong Chen Kunmiao Wang Yingying Qian Luoping Wang Jing Li Qili Mi Haitao Huang Li Xu Mingfang Zhao Yingzhen Zhang Haiying Xiang |
| author_facet | Wanli Zeng Dan Xiang Xuemei Li Qian Gao Yudong Chen Kunmiao Wang Yingying Qian Luoping Wang Jing Li Qili Mi Haitao Huang Li Xu Mingfang Zhao Yingzhen Zhang Haiying Xiang |
| author_sort | Wanli Zeng |
| collection | DOAJ |
| description | The effects of rhizosphere microorganisms on plant growth and the associated mechanisms are a focus of current research, but the effects of exogenous combined inoculation with arbuscular mycorrhizal fungi (AMF) and plant growth-promoting rhizobacteria (PGPR) on seedling growth and the associated rhizosphere microecological mechanisms have been little reported. In this study, a greenhouse pot experiment was used to study the effects of single or double inoculation with AM fungi (Funneliformis mosseae) and two PGPR (Bacillus sp., Pseudomonas sp.) on the growth of tobacco seedlings, together with high-throughput sequencing technology to reveal associated rhizosphere microecological mechanisms. All inoculation treatments significantly increased the aboveground dry weight; root dry weight; seedling nitrogen, phosphorus, and potassium uptake; plant height; stem thickness; maximum leaf area; chlorophyll content; total root length, surface area, and volume; and average root diameter. The highest values for these indices were observed in the combined treatment of F. mosseae and Pseudomonas sp. SG29 (A_SG29). Furthermore, the A_SG29 treatment yielded the highest diversity indexes and largest percentages of significantly enriched bacterial taxa, and significantly promoted the colonization of AMF in tobacco roots and Pseudomonas in rhizosphere soil. Differential metabolic-pathway predictions using PICRUSt2 showed that the A_SG29 treatment significantly increased the metabolic pathway richness of tobacco rhizosphere microorganisms, and significantly up-regulated some metabolic pathways that may benefit plant growth. Co-inoculation with F. mosseae and Pseudomonas sp. SG29 promoted tobacco-seedling growth by significantly improving rhizosphere microbial communities' structure and function. In summary, the combined inoculation of AMF and SG29 promotes tobacco seedling growth, optimizes the rhizosphere microbial community's structure and function, and serves as a sustainable microbial co-cultivation method for tobacco seedling production. |
| format | Article |
| id | doaj-art-43fbb640fb2c4d14987d03146f652b10 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-43fbb640fb2c4d14987d03146f652b102025-01-24T16:08:10ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011510.3389/fmicb.2024.14754851475485Effects of combined inoculation of arbuscular mycorrhizal fungi and plant growth-promoting rhizosphere bacteria on seedling growth and rhizosphere microecologyWanli Zeng0Dan Xiang1Xuemei Li2Qian Gao3Yudong Chen4Kunmiao Wang5Yingying Qian6Luoping Wang7Jing Li8Qili Mi9Haitao Huang10Li Xu11Mingfang Zhao12Yingzhen Zhang13Haiying Xiang14Yunnan Academy of Tobacco Science, Kunming, ChinaCollege of Resources and Environment, Qingdao Agricultural University, Qingdao, ChinaYunnan Academy of Tobacco Science, Kunming, ChinaYunnan Academy of Tobacco Science, Kunming, ChinaYunnan Academy of Tobacco Science, Kunming, ChinaYunnan Academy of Tobacco Science, Kunming, ChinaYunnan Academy of Tobacco Science, Kunming, ChinaYunnan Academy of Tobacco Science, Kunming, ChinaYunnan Academy of Tobacco Science, Kunming, ChinaYunnan Academy of Tobacco Science, Kunming, ChinaYunnan Academy of Tobacco Science, Kunming, ChinaYunnan Academy of Tobacco Science, Kunming, ChinaCollege of Resources and Environment, Qingdao Agricultural University, Qingdao, ChinaCollege of Resources and Environment, Qingdao Agricultural University, Qingdao, ChinaYunnan Academy of Tobacco Science, Kunming, ChinaThe effects of rhizosphere microorganisms on plant growth and the associated mechanisms are a focus of current research, but the effects of exogenous combined inoculation with arbuscular mycorrhizal fungi (AMF) and plant growth-promoting rhizobacteria (PGPR) on seedling growth and the associated rhizosphere microecological mechanisms have been little reported. In this study, a greenhouse pot experiment was used to study the effects of single or double inoculation with AM fungi (Funneliformis mosseae) and two PGPR (Bacillus sp., Pseudomonas sp.) on the growth of tobacco seedlings, together with high-throughput sequencing technology to reveal associated rhizosphere microecological mechanisms. All inoculation treatments significantly increased the aboveground dry weight; root dry weight; seedling nitrogen, phosphorus, and potassium uptake; plant height; stem thickness; maximum leaf area; chlorophyll content; total root length, surface area, and volume; and average root diameter. The highest values for these indices were observed in the combined treatment of F. mosseae and Pseudomonas sp. SG29 (A_SG29). Furthermore, the A_SG29 treatment yielded the highest diversity indexes and largest percentages of significantly enriched bacterial taxa, and significantly promoted the colonization of AMF in tobacco roots and Pseudomonas in rhizosphere soil. Differential metabolic-pathway predictions using PICRUSt2 showed that the A_SG29 treatment significantly increased the metabolic pathway richness of tobacco rhizosphere microorganisms, and significantly up-regulated some metabolic pathways that may benefit plant growth. Co-inoculation with F. mosseae and Pseudomonas sp. SG29 promoted tobacco-seedling growth by significantly improving rhizosphere microbial communities' structure and function. In summary, the combined inoculation of AMF and SG29 promotes tobacco seedling growth, optimizes the rhizosphere microbial community's structure and function, and serves as a sustainable microbial co-cultivation method for tobacco seedling production.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1475485/fullarbuscular mycorrhizal fungigrowth attributesmicroecological mechanismsplant growth-promoting rhizobacteriaillumina sequencing |
| spellingShingle | Wanli Zeng Dan Xiang Xuemei Li Qian Gao Yudong Chen Kunmiao Wang Yingying Qian Luoping Wang Jing Li Qili Mi Haitao Huang Li Xu Mingfang Zhao Yingzhen Zhang Haiying Xiang Effects of combined inoculation of arbuscular mycorrhizal fungi and plant growth-promoting rhizosphere bacteria on seedling growth and rhizosphere microecology Frontiers in Microbiology arbuscular mycorrhizal fungi growth attributes microecological mechanisms plant growth-promoting rhizobacteria illumina sequencing |
| title | Effects of combined inoculation of arbuscular mycorrhizal fungi and plant growth-promoting rhizosphere bacteria on seedling growth and rhizosphere microecology |
| title_full | Effects of combined inoculation of arbuscular mycorrhizal fungi and plant growth-promoting rhizosphere bacteria on seedling growth and rhizosphere microecology |
| title_fullStr | Effects of combined inoculation of arbuscular mycorrhizal fungi and plant growth-promoting rhizosphere bacteria on seedling growth and rhizosphere microecology |
| title_full_unstemmed | Effects of combined inoculation of arbuscular mycorrhizal fungi and plant growth-promoting rhizosphere bacteria on seedling growth and rhizosphere microecology |
| title_short | Effects of combined inoculation of arbuscular mycorrhizal fungi and plant growth-promoting rhizosphere bacteria on seedling growth and rhizosphere microecology |
| title_sort | effects of combined inoculation of arbuscular mycorrhizal fungi and plant growth promoting rhizosphere bacteria on seedling growth and rhizosphere microecology |
| topic | arbuscular mycorrhizal fungi growth attributes microecological mechanisms plant growth-promoting rhizobacteria illumina sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1475485/full |
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