Genomic and Drug Resistance Profile of Bovine Multidrug-Resistant <i>Escherichia coli</i> Isolated in Kazakhstan
While studying the prevalence and profile of antibiotic resistance among <i>E. coli</i> isolated from the feces of calves with signs of colibacillosis, a strain with a wide spectrum of drug resistance was isolated. Whole-genome sequencing, followed by bioinformatic processing and the ann...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/14/1/90 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587764036534272 |
|---|---|
| author | Pavel G. Alexyuk Andrey P. Bogoyavlenskiy Yergali S. Moldakhanov Kuralay S. Akanova Adolat N. Manakbayeva Timur Kerimov Vladimir E. Berezin Madina S. Alexyuk |
| author_facet | Pavel G. Alexyuk Andrey P. Bogoyavlenskiy Yergali S. Moldakhanov Kuralay S. Akanova Adolat N. Manakbayeva Timur Kerimov Vladimir E. Berezin Madina S. Alexyuk |
| author_sort | Pavel G. Alexyuk |
| collection | DOAJ |
| description | While studying the prevalence and profile of antibiotic resistance among <i>E. coli</i> isolated from the feces of calves with signs of colibacillosis, a strain with a wide spectrum of drug resistance was isolated. Whole-genome sequencing, followed by bioinformatic processing and the annotation of genes of this strain, showed that the genome has a total length of 4,803,482 bp and contains 4986 genes, including 122 RNA genes. A total of 31% of the genes are functionally significant and represent 26 functional groups. Additionally, 55 antibiotic resistance genes were revealed. A phenotypic drug-resistance study was performed according to CASFM and CLSI guidelines, which showed that the investigated strain was resistant to eight antibacterial drugs of different classes, including colistin. This is the first report on the AMR profile of an <i>E. coli</i> isolate obtained from a sick calf with evidence of escherichiosis in Kazakhstan. The provided information on the genome will be valuable in studying the evolution and development of antibiotic-resistant forms of <i>E. coli</i> and increase our knowledge of pathogenicity. It may also be a source for future comparative studies of the virulence and drug resistance of <i>E. coli</i> isolates. |
| format | Article |
| id | doaj-art-43f77f99f02f4696a9da6f463d575a42 |
| institution | Kabale University |
| issn | 2076-0817 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj-art-43f77f99f02f4696a9da6f463d575a422025-01-24T13:44:52ZengMDPI AGPathogens2076-08172025-01-011419010.3390/pathogens14010090Genomic and Drug Resistance Profile of Bovine Multidrug-Resistant <i>Escherichia coli</i> Isolated in KazakhstanPavel G. Alexyuk0Andrey P. Bogoyavlenskiy1Yergali S. Moldakhanov2Kuralay S. Akanova3Adolat N. Manakbayeva4Timur Kerimov5Vladimir E. Berezin6Madina S. Alexyuk7Research and Production Center for Microbiology and Virology, Almaty 050010, KazakhstanResearch and Production Center for Microbiology and Virology, Almaty 050010, KazakhstanResearch and Production Center for Microbiology and Virology, Almaty 050010, KazakhstanResearch and Production Center for Microbiology and Virology, Almaty 050010, KazakhstanResearch and Production Center for Microbiology and Virology, Almaty 050010, KazakhstanResearch and Production Center for Microbiology and Virology, Almaty 050010, KazakhstanResearch and Production Center for Microbiology and Virology, Almaty 050010, KazakhstanResearch and Production Center for Microbiology and Virology, Almaty 050010, KazakhstanWhile studying the prevalence and profile of antibiotic resistance among <i>E. coli</i> isolated from the feces of calves with signs of colibacillosis, a strain with a wide spectrum of drug resistance was isolated. Whole-genome sequencing, followed by bioinformatic processing and the annotation of genes of this strain, showed that the genome has a total length of 4,803,482 bp and contains 4986 genes, including 122 RNA genes. A total of 31% of the genes are functionally significant and represent 26 functional groups. Additionally, 55 antibiotic resistance genes were revealed. A phenotypic drug-resistance study was performed according to CASFM and CLSI guidelines, which showed that the investigated strain was resistant to eight antibacterial drugs of different classes, including colistin. This is the first report on the AMR profile of an <i>E. coli</i> isolate obtained from a sick calf with evidence of escherichiosis in Kazakhstan. The provided information on the genome will be valuable in studying the evolution and development of antibiotic-resistant forms of <i>E. coli</i> and increase our knowledge of pathogenicity. It may also be a source for future comparative studies of the virulence and drug resistance of <i>E. coli</i> isolates.https://www.mdpi.com/2076-0817/14/1/90<i>Escherichia coli</i>multidrug resistancecolibacillosisdraft genomeIllumina sequencing |
| spellingShingle | Pavel G. Alexyuk Andrey P. Bogoyavlenskiy Yergali S. Moldakhanov Kuralay S. Akanova Adolat N. Manakbayeva Timur Kerimov Vladimir E. Berezin Madina S. Alexyuk Genomic and Drug Resistance Profile of Bovine Multidrug-Resistant <i>Escherichia coli</i> Isolated in Kazakhstan Pathogens <i>Escherichia coli</i> multidrug resistance colibacillosis draft genome Illumina sequencing |
| title | Genomic and Drug Resistance Profile of Bovine Multidrug-Resistant <i>Escherichia coli</i> Isolated in Kazakhstan |
| title_full | Genomic and Drug Resistance Profile of Bovine Multidrug-Resistant <i>Escherichia coli</i> Isolated in Kazakhstan |
| title_fullStr | Genomic and Drug Resistance Profile of Bovine Multidrug-Resistant <i>Escherichia coli</i> Isolated in Kazakhstan |
| title_full_unstemmed | Genomic and Drug Resistance Profile of Bovine Multidrug-Resistant <i>Escherichia coli</i> Isolated in Kazakhstan |
| title_short | Genomic and Drug Resistance Profile of Bovine Multidrug-Resistant <i>Escherichia coli</i> Isolated in Kazakhstan |
| title_sort | genomic and drug resistance profile of bovine multidrug resistant i escherichia coli i isolated in kazakhstan |
| topic | <i>Escherichia coli</i> multidrug resistance colibacillosis draft genome Illumina sequencing |
| url | https://www.mdpi.com/2076-0817/14/1/90 |
| work_keys_str_mv | AT pavelgalexyuk genomicanddrugresistanceprofileofbovinemultidrugresistantiescherichiacoliiisolatedinkazakhstan AT andreypbogoyavlenskiy genomicanddrugresistanceprofileofbovinemultidrugresistantiescherichiacoliiisolatedinkazakhstan AT yergalismoldakhanov genomicanddrugresistanceprofileofbovinemultidrugresistantiescherichiacoliiisolatedinkazakhstan AT kuralaysakanova genomicanddrugresistanceprofileofbovinemultidrugresistantiescherichiacoliiisolatedinkazakhstan AT adolatnmanakbayeva genomicanddrugresistanceprofileofbovinemultidrugresistantiescherichiacoliiisolatedinkazakhstan AT timurkerimov genomicanddrugresistanceprofileofbovinemultidrugresistantiescherichiacoliiisolatedinkazakhstan AT vladimireberezin genomicanddrugresistanceprofileofbovinemultidrugresistantiescherichiacoliiisolatedinkazakhstan AT madinasalexyuk genomicanddrugresistanceprofileofbovinemultidrugresistantiescherichiacoliiisolatedinkazakhstan |