Antimicrobial peptide production with Corynebacterium glutamicum on lignocellulosic side streams
Abstract Background Biorefineries usually focus on the production of low-value commodities, such as bioethanol, platform chemicals or single cell protein. Shifting production to bioactive compounds, such as antimicrobial peptides, could provide an opportunity to increase the economic viability of bi...
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| Format: | Article |
| Language: | English |
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BMC
2024-12-01
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| Series: | Biotechnology for Biofuels and Bioproducts |
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| Online Access: | https://doi.org/10.1186/s13068-024-02587-1 |
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| author | Daniel Waldschitz Mark-Richard Neudert Jakob Kitzmüller Jae Hwi Bong Yannick Bus Eva Maria Karner Peter Sinner Oliver Spadiut |
| author_facet | Daniel Waldschitz Mark-Richard Neudert Jakob Kitzmüller Jae Hwi Bong Yannick Bus Eva Maria Karner Peter Sinner Oliver Spadiut |
| author_sort | Daniel Waldschitz |
| collection | DOAJ |
| description | Abstract Background Biorefineries usually focus on the production of low-value commodities, such as bioethanol, platform chemicals or single cell protein. Shifting production to bioactive compounds, such as antimicrobial peptides, could provide an opportunity to increase the economic viability of biorefineries. Results Recombinant production of the antimicrobial peptide pediocin PA-1 in Corynebacterium glutamicum was transferred from yeast extract-based media to minimal media based on lignocellulosic spent sulfite liquor. Induced batch, fed batch, and extended batch process modes were compared for highest pediocin PA-1 production. Conclusion For pediocin PA-1 production on lignocellulosic residues, extended batch cultivation was identified as the optimal process mode, producing up to $$\simeq$$ ≃ 104 mg/L active pediocin PA-1. Moreover, the production of pediocin PA-1 on this sustainable second generation resource exceeded its state-of-the-art production on yeast extract-based media $$\simeq$$ ≃ 1.5-fold. |
| format | Article |
| id | doaj-art-43f539f30c4b46f5a9cbe4b2e26604f7 |
| institution | OA Journals |
| issn | 2731-3654 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj-art-43f539f30c4b46f5a9cbe4b2e26604f72025-08-20T02:31:41ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542024-12-0117111110.1186/s13068-024-02587-1 Antimicrobial peptide production with Corynebacterium glutamicum on lignocellulosic side streamsDaniel Waldschitz0Mark-Richard Neudert1Jakob Kitzmüller2Jae Hwi Bong3Yannick Bus4Eva Maria Karner5Peter Sinner6Oliver Spadiut7Research Group Bioprocess Technology, Institute of Chemical, Environmental and Bioscience Engineering, TU WienResearch Group Bioprocess Technology, Institute of Chemical, Environmental and Bioscience Engineering, TU WienResearch Group Bioprocess Technology, Institute of Chemical, Environmental and Bioscience Engineering, TU WienResearch Group Bioprocess Technology, Institute of Chemical, Environmental and Bioscience Engineering, TU WienResearch Group Bioprocess Technology, Institute of Chemical, Environmental and Bioscience Engineering, TU WienResearch Group Bioprocess Technology, Institute of Chemical, Environmental and Bioscience Engineering, TU WienResearch Group Bioprocess Technology, Institute of Chemical, Environmental and Bioscience Engineering, TU WienResearch Group Bioprocess Technology, Institute of Chemical, Environmental and Bioscience Engineering, TU WienAbstract Background Biorefineries usually focus on the production of low-value commodities, such as bioethanol, platform chemicals or single cell protein. Shifting production to bioactive compounds, such as antimicrobial peptides, could provide an opportunity to increase the economic viability of biorefineries. Results Recombinant production of the antimicrobial peptide pediocin PA-1 in Corynebacterium glutamicum was transferred from yeast extract-based media to minimal media based on lignocellulosic spent sulfite liquor. Induced batch, fed batch, and extended batch process modes were compared for highest pediocin PA-1 production. Conclusion For pediocin PA-1 production on lignocellulosic residues, extended batch cultivation was identified as the optimal process mode, producing up to $$\simeq$$ ≃ 104 mg/L active pediocin PA-1. Moreover, the production of pediocin PA-1 on this sustainable second generation resource exceeded its state-of-the-art production on yeast extract-based media $$\simeq$$ ≃ 1.5-fold.https://doi.org/10.1186/s13068-024-02587-1Lignocellulosic feedstockSpent sulfite liquorAntimicrobial peptideRecombinant productionPediocin PA-1Corynebacterium glutamicum |
| spellingShingle | Daniel Waldschitz Mark-Richard Neudert Jakob Kitzmüller Jae Hwi Bong Yannick Bus Eva Maria Karner Peter Sinner Oliver Spadiut Antimicrobial peptide production with Corynebacterium glutamicum on lignocellulosic side streams Biotechnology for Biofuels and Bioproducts Lignocellulosic feedstock Spent sulfite liquor Antimicrobial peptide Recombinant production Pediocin PA-1 Corynebacterium glutamicum |
| title | Antimicrobial peptide production with Corynebacterium glutamicum on lignocellulosic side streams |
| title_full | Antimicrobial peptide production with Corynebacterium glutamicum on lignocellulosic side streams |
| title_fullStr | Antimicrobial peptide production with Corynebacterium glutamicum on lignocellulosic side streams |
| title_full_unstemmed | Antimicrobial peptide production with Corynebacterium glutamicum on lignocellulosic side streams |
| title_short | Antimicrobial peptide production with Corynebacterium glutamicum on lignocellulosic side streams |
| title_sort | antimicrobial peptide production with corynebacterium glutamicum on lignocellulosic side streams |
| topic | Lignocellulosic feedstock Spent sulfite liquor Antimicrobial peptide Recombinant production Pediocin PA-1 Corynebacterium glutamicum |
| url | https://doi.org/10.1186/s13068-024-02587-1 |
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