Study on the physiological mechanism and molecular regulatory network of Blumea balsamifera in response to drought stress
Abstract Drought restricts plant growth and agricultural production. As an important medicinal plant, Blumea balsamifera is sensitive to water, but there is still a lack of systematic research on its drought response mechanism. In this study, four-month-old B. balsamifera seedlings were used as mate...
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BMC
2025-07-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-025-06916-w |
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| author | Changmao Guo Zejun Mo Su Chen Kailang Mu Shi Yao Qiumei Luo Zhengwei Zhang Tianjian Wang Gang Liu Yuchen Liu Yuxin Pang |
| author_facet | Changmao Guo Zejun Mo Su Chen Kailang Mu Shi Yao Qiumei Luo Zhengwei Zhang Tianjian Wang Gang Liu Yuchen Liu Yuxin Pang |
| author_sort | Changmao Guo |
| collection | DOAJ |
| description | Abstract Drought restricts plant growth and agricultural production. As an important medicinal plant, Blumea balsamifera is sensitive to water, but there is still a lack of systematic research on its drought response mechanism. In this study, four-month-old B. balsamifera seedlings were used as materials, and three groups were set up: normal irrigation (CK), drought stress (DS), and rewatering recovery (RW). The results showed that drought significantly inhibited the growth and photosynthesis of B. balsamifera. With the prolongation of stress time (day 12), the limiting factor of photosynthesis changed from initial stomatal limitation to non - stomatal limitation. In terms of physiology and biochemistry, B. balsamifera increased MDA content by actively reducing SPAD value and relative water content of leaves; and activates the antioxidant enzyme system to remove ROS, synergistically accumulates lignin, soluble sugar, proline and other osmotic adjustment substances, and jointly maintains cell water balance and membrane system stability. Through transcriptome and proteome analysis, 20,874 DEGs and 2770 DEPs were screened out, which were significantly enriched in terms related to ribosome, oxidoreductase activity, biosynthesis of unsaturated fatty acids and other pathways. A total of 55 drought - related DEGs - DEPs were identified by two - omics, and 18 key regulatory genes were screened. In summary, B. balsamifera formed a comprehensive drought resistance mechanism through photosynthesis, physiology and DEGs - DEPs network. This study provides theoretical support for the breeding and resource development of B. balsamifera, and also provides reference for the study of stress resistance of other medicinal plants. |
| format | Article |
| id | doaj-art-43e0197a61a7490d891f5ca4b158c33c |
| institution | Kabale University |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-43e0197a61a7490d891f5ca4b158c33c2025-08-20T03:37:20ZengBMCBMC Plant Biology1471-22292025-07-0125111710.1186/s12870-025-06916-wStudy on the physiological mechanism and molecular regulatory network of Blumea balsamifera in response to drought stressChangmao Guo0Zejun Mo1Su Chen2Kailang Mu3Shi Yao4Qiumei Luo5Zhengwei Zhang6Tianjian Wang7Gang Liu8Yuchen Liu9Yuxin Pang10College of Pharmacy, Guizhou University of Traditional Chinese MedicineCollege of Pharmacy, Guizhou University of Traditional Chinese MedicineCollege of Pharmacy, Guizhou University of Traditional Chinese MedicineCollege of Pharmacy, Guizhou University of Traditional Chinese MedicineCollege of Pharmacy, Guizhou University of Traditional Chinese MedicineCollege of Pharmacy, Guizhou University of Traditional Chinese MedicineCollege of Pharmacy, Guizhou University of Traditional Chinese MedicineCollege of Pharmacy, Guizhou University of Traditional Chinese MedicineCollege of Pharmacy, Guizhou University of Traditional Chinese MedicineCollege of Pharmacy, Guizhou University of Traditional Chinese MedicineCollege of Pharmacy, Guizhou University of Traditional Chinese MedicineAbstract Drought restricts plant growth and agricultural production. As an important medicinal plant, Blumea balsamifera is sensitive to water, but there is still a lack of systematic research on its drought response mechanism. In this study, four-month-old B. balsamifera seedlings were used as materials, and three groups were set up: normal irrigation (CK), drought stress (DS), and rewatering recovery (RW). The results showed that drought significantly inhibited the growth and photosynthesis of B. balsamifera. With the prolongation of stress time (day 12), the limiting factor of photosynthesis changed from initial stomatal limitation to non - stomatal limitation. In terms of physiology and biochemistry, B. balsamifera increased MDA content by actively reducing SPAD value and relative water content of leaves; and activates the antioxidant enzyme system to remove ROS, synergistically accumulates lignin, soluble sugar, proline and other osmotic adjustment substances, and jointly maintains cell water balance and membrane system stability. Through transcriptome and proteome analysis, 20,874 DEGs and 2770 DEPs were screened out, which were significantly enriched in terms related to ribosome, oxidoreductase activity, biosynthesis of unsaturated fatty acids and other pathways. A total of 55 drought - related DEGs - DEPs were identified by two - omics, and 18 key regulatory genes were screened. In summary, B. balsamifera formed a comprehensive drought resistance mechanism through photosynthesis, physiology and DEGs - DEPs network. This study provides theoretical support for the breeding and resource development of B. balsamifera, and also provides reference for the study of stress resistance of other medicinal plants.https://doi.org/10.1186/s12870-025-06916-wDrought stressDrought resistance mechanismTranscriptomicsProteomicsBlumea balsamifera L |
| spellingShingle | Changmao Guo Zejun Mo Su Chen Kailang Mu Shi Yao Qiumei Luo Zhengwei Zhang Tianjian Wang Gang Liu Yuchen Liu Yuxin Pang Study on the physiological mechanism and molecular regulatory network of Blumea balsamifera in response to drought stress BMC Plant Biology Drought stress Drought resistance mechanism Transcriptomics Proteomics Blumea balsamifera L |
| title | Study on the physiological mechanism and molecular regulatory network of Blumea balsamifera in response to drought stress |
| title_full | Study on the physiological mechanism and molecular regulatory network of Blumea balsamifera in response to drought stress |
| title_fullStr | Study on the physiological mechanism and molecular regulatory network of Blumea balsamifera in response to drought stress |
| title_full_unstemmed | Study on the physiological mechanism and molecular regulatory network of Blumea balsamifera in response to drought stress |
| title_short | Study on the physiological mechanism and molecular regulatory network of Blumea balsamifera in response to drought stress |
| title_sort | study on the physiological mechanism and molecular regulatory network of blumea balsamifera in response to drought stress |
| topic | Drought stress Drought resistance mechanism Transcriptomics Proteomics Blumea balsamifera L |
| url | https://doi.org/10.1186/s12870-025-06916-w |
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