Application of Metagenomic Next-Generation Sequencing in the Diagnosis of Infectious Keratitis
Purpose. To determine the advantages of next-generation metagenomic sequencing (mNGS) technology in the diagnosis and treatment of infectious keratitis (IK). Methods. A total of 287 patients with IK admitted to the Department of Ophthalmology of Nanjing First Hospital between August 2018 and Decembe...
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| Language: | English |
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Wiley
2024-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2024/9911979 |
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| author | Xin-Yu Pan Meng Wang Yi-Dan Xu Lin-Nong Wang |
| author_facet | Xin-Yu Pan Meng Wang Yi-Dan Xu Lin-Nong Wang |
| author_sort | Xin-Yu Pan |
| collection | DOAJ |
| description | Purpose. To determine the advantages of next-generation metagenomic sequencing (mNGS) technology in the diagnosis and treatment of infectious keratitis (IK). Methods. A total of 287 patients with IK admitted to the Department of Ophthalmology of Nanjing First Hospital between August 2018 and December 2022 were analyzed retrospectively, and the pathogenic causes, etiological characteristics, detection, treatment methods, and efficacy were summarized. Results. Trauma and foreign matter were the most common causes of IK (144 patients, 50.2%). Of the 287 patients, 228 (79.4%) were diagnosed with a specific etiology, including 110 (48.2%) fungal infections, 44 (19.3%) viral infections, 42 (18.4%) mixed infections, and 30 (13.2%) bacterial infections. Filamentous fungi represented by Fusarium and Aspergillus were the most common, followed by bacteria such as Pseudomonas aeruginosa, Streptococcus pneumoniae, viruses (Herpes Simplex Virus/Varicella-Zoster Virus), and parasites. The positivity rates of secretion culture, corneal laser confocal microscopy (CM), mNGS, and pathological sections were 47.3% (133/281), 45.3% (111/245), 83.9% (104/124), and 19.3% (40/207), respectively. The positivity rate of mNGS for bacteria and viruses was higher than that of the other methods, and the positivity rate for fungi was the same as that for CM. As a result, 214 cases (74.6%) were cured, 51 cases (17.8%) improved, 8 cases (2.8%) did not heal, ocular content enucleation was performed in 14 cases (4.9%), and the overall efficacy rate was 92.3%. Conclusion. Trauma and foreign matter are the main causes of IK. The mNGS technology is an efficient and comprehensive detection method for viruses and bacteria, especially for mixed infections. |
| format | Article |
| id | doaj-art-43dd50bed6c74793a7ee4fd584493e60 |
| institution | Kabale University |
| issn | 2090-0058 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-43dd50bed6c74793a7ee4fd584493e602025-02-03T05:56:54ZengWileyJournal of Ophthalmology2090-00582024-01-01202410.1155/2024/9911979Application of Metagenomic Next-Generation Sequencing in the Diagnosis of Infectious KeratitisXin-Yu Pan0Meng Wang1Yi-Dan Xu2Lin-Nong Wang3Department of OphthalmologyDepartment of OphthalmologyDepartment of OphthalmologyDepartment of OphthalmologyPurpose. To determine the advantages of next-generation metagenomic sequencing (mNGS) technology in the diagnosis and treatment of infectious keratitis (IK). Methods. A total of 287 patients with IK admitted to the Department of Ophthalmology of Nanjing First Hospital between August 2018 and December 2022 were analyzed retrospectively, and the pathogenic causes, etiological characteristics, detection, treatment methods, and efficacy were summarized. Results. Trauma and foreign matter were the most common causes of IK (144 patients, 50.2%). Of the 287 patients, 228 (79.4%) were diagnosed with a specific etiology, including 110 (48.2%) fungal infections, 44 (19.3%) viral infections, 42 (18.4%) mixed infections, and 30 (13.2%) bacterial infections. Filamentous fungi represented by Fusarium and Aspergillus were the most common, followed by bacteria such as Pseudomonas aeruginosa, Streptococcus pneumoniae, viruses (Herpes Simplex Virus/Varicella-Zoster Virus), and parasites. The positivity rates of secretion culture, corneal laser confocal microscopy (CM), mNGS, and pathological sections were 47.3% (133/281), 45.3% (111/245), 83.9% (104/124), and 19.3% (40/207), respectively. The positivity rate of mNGS for bacteria and viruses was higher than that of the other methods, and the positivity rate for fungi was the same as that for CM. As a result, 214 cases (74.6%) were cured, 51 cases (17.8%) improved, 8 cases (2.8%) did not heal, ocular content enucleation was performed in 14 cases (4.9%), and the overall efficacy rate was 92.3%. Conclusion. Trauma and foreign matter are the main causes of IK. The mNGS technology is an efficient and comprehensive detection method for viruses and bacteria, especially for mixed infections.http://dx.doi.org/10.1155/2024/9911979 |
| spellingShingle | Xin-Yu Pan Meng Wang Yi-Dan Xu Lin-Nong Wang Application of Metagenomic Next-Generation Sequencing in the Diagnosis of Infectious Keratitis Journal of Ophthalmology |
| title | Application of Metagenomic Next-Generation Sequencing in the Diagnosis of Infectious Keratitis |
| title_full | Application of Metagenomic Next-Generation Sequencing in the Diagnosis of Infectious Keratitis |
| title_fullStr | Application of Metagenomic Next-Generation Sequencing in the Diagnosis of Infectious Keratitis |
| title_full_unstemmed | Application of Metagenomic Next-Generation Sequencing in the Diagnosis of Infectious Keratitis |
| title_short | Application of Metagenomic Next-Generation Sequencing in the Diagnosis of Infectious Keratitis |
| title_sort | application of metagenomic next generation sequencing in the diagnosis of infectious keratitis |
| url | http://dx.doi.org/10.1155/2024/9911979 |
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