Transcriptome analysis reveals potential regulatory mechanism of genes and pathways following Aeromonas veronii infection and hypoxic stress in Chinese mitten crab, Eriocheir sinensis
Environment hypoxia and Aeromonas veronii infection represent the major physiological challenge for the economic aquaculture species. However, the molecular mechanisms underlying these stresses remain incompletely elucidated. This study focused on evaluating hypoxia stress and A. veronii infection i...
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006951 |
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| author | Gang Jiang Fengyuan Yan Yuanfeng Xu Jianlin Li Wenrong Feng Guo an Hua Wen jing Li Jun Zhou Yongkai Tang |
| author_facet | Gang Jiang Fengyuan Yan Yuanfeng Xu Jianlin Li Wenrong Feng Guo an Hua Wen jing Li Jun Zhou Yongkai Tang |
| author_sort | Gang Jiang |
| collection | DOAJ |
| description | Environment hypoxia and Aeromonas veronii infection represent the major physiological challenge for the economic aquaculture species. However, the molecular mechanisms underlying these stresses remain incompletely elucidated. This study focused on evaluating hypoxia stress and A. veronii infection in Eriocheir sinensis, and elucidating the molecular mechanisms via biochemical, histopathological and transcriptomic analyses. 360 healthy E. sinensis were equally divided into four groups: a normoxic control (C) at 7.5 ± 0.5 mg/L DO, a hypoxic treatment (Th) at 1.5 ± 0.5 mg/L DO, an A. veronii-infected group (Tv) at 3.5 × 107 CFU/mL under normal DO conditions, and a dual-stress condition (Thv) combining hypoxia and infection. Results demonstrated significantly elevated levels of antioxidant enzymes (T-AOC, SOD, and CAT), and immune enzymes (AKP, ACP) in treatments Th, Tv, and Thv, with a contrasting decrease in MDA contents. Moreover, structural damage in hepatopancreas and gills, notably vacuolation and lobular membrane thickening among these treatments. Transcriptome analysis highlighted 157, 135 and 161 differentially expressed genes (DEGs) in treatments Th, Tv, and Thv, respectively, relative to C. KEGG pathway analysis indicated these DEGs were predominantly associated with pathways linked to immunity and metabolism. Hypoxia activated genes involved in energy metabolism while down-regulated those related to protein synthesis. A. veronii infection, meanwhile, reduced the expression of genes associated with lipid and nitrogen metabolism. Under dual stress, protein synthesis and lipid metabolism were further repressed, and immune-related gene expression was markedly up-regulated. Notably, hypoxia and A. veronii infection exhibit a synergistic toxic effect on E. sinensis. Finally, 29 DEGs were identified as critical in responding to hypoxia stress and A. veronii infection. These findings contribute to understanding the mechanisms of stress responses in aquatic animals, potentially aiding in the development of strategies to enhance health and productivity in aquaculture. |
| format | Article |
| id | doaj-art-43b39303f2fd4841ad8f3acec2c4c638 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-43b39303f2fd4841ad8f3acec2c4c6382025-02-06T05:12:17ZengElsevierAquaculture Reports2352-51342025-03-0140102607Transcriptome analysis reveals potential regulatory mechanism of genes and pathways following Aeromonas veronii infection and hypoxic stress in Chinese mitten crab, Eriocheir sinensisGang Jiang0Fengyuan Yan1Yuanfeng Xu2Jianlin Li3Wenrong Feng4Guo an Hua5Wen jing Li6Jun Zhou7Yongkai Tang8Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaJiangsu Haorun Biological Industry Group Co., Ltd, Taizhou 225309, ChinaJiangsu Haorun Biological Industry Group Co., Ltd, Taizhou 225309, ChinaFreshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; Corresponding author at: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.Environment hypoxia and Aeromonas veronii infection represent the major physiological challenge for the economic aquaculture species. However, the molecular mechanisms underlying these stresses remain incompletely elucidated. This study focused on evaluating hypoxia stress and A. veronii infection in Eriocheir sinensis, and elucidating the molecular mechanisms via biochemical, histopathological and transcriptomic analyses. 360 healthy E. sinensis were equally divided into four groups: a normoxic control (C) at 7.5 ± 0.5 mg/L DO, a hypoxic treatment (Th) at 1.5 ± 0.5 mg/L DO, an A. veronii-infected group (Tv) at 3.5 × 107 CFU/mL under normal DO conditions, and a dual-stress condition (Thv) combining hypoxia and infection. Results demonstrated significantly elevated levels of antioxidant enzymes (T-AOC, SOD, and CAT), and immune enzymes (AKP, ACP) in treatments Th, Tv, and Thv, with a contrasting decrease in MDA contents. Moreover, structural damage in hepatopancreas and gills, notably vacuolation and lobular membrane thickening among these treatments. Transcriptome analysis highlighted 157, 135 and 161 differentially expressed genes (DEGs) in treatments Th, Tv, and Thv, respectively, relative to C. KEGG pathway analysis indicated these DEGs were predominantly associated with pathways linked to immunity and metabolism. Hypoxia activated genes involved in energy metabolism while down-regulated those related to protein synthesis. A. veronii infection, meanwhile, reduced the expression of genes associated with lipid and nitrogen metabolism. Under dual stress, protein synthesis and lipid metabolism were further repressed, and immune-related gene expression was markedly up-regulated. Notably, hypoxia and A. veronii infection exhibit a synergistic toxic effect on E. sinensis. Finally, 29 DEGs were identified as critical in responding to hypoxia stress and A. veronii infection. These findings contribute to understanding the mechanisms of stress responses in aquatic animals, potentially aiding in the development of strategies to enhance health and productivity in aquaculture.http://www.sciencedirect.com/science/article/pii/S2352513424006951Chinese mitten crabHypoxic stressAeromonas veronii infectionImmunityMetabolism |
| spellingShingle | Gang Jiang Fengyuan Yan Yuanfeng Xu Jianlin Li Wenrong Feng Guo an Hua Wen jing Li Jun Zhou Yongkai Tang Transcriptome analysis reveals potential regulatory mechanism of genes and pathways following Aeromonas veronii infection and hypoxic stress in Chinese mitten crab, Eriocheir sinensis Aquaculture Reports Chinese mitten crab Hypoxic stress Aeromonas veronii infection Immunity Metabolism |
| title | Transcriptome analysis reveals potential regulatory mechanism of genes and pathways following Aeromonas veronii infection and hypoxic stress in Chinese mitten crab, Eriocheir sinensis |
| title_full | Transcriptome analysis reveals potential regulatory mechanism of genes and pathways following Aeromonas veronii infection and hypoxic stress in Chinese mitten crab, Eriocheir sinensis |
| title_fullStr | Transcriptome analysis reveals potential regulatory mechanism of genes and pathways following Aeromonas veronii infection and hypoxic stress in Chinese mitten crab, Eriocheir sinensis |
| title_full_unstemmed | Transcriptome analysis reveals potential regulatory mechanism of genes and pathways following Aeromonas veronii infection and hypoxic stress in Chinese mitten crab, Eriocheir sinensis |
| title_short | Transcriptome analysis reveals potential regulatory mechanism of genes and pathways following Aeromonas veronii infection and hypoxic stress in Chinese mitten crab, Eriocheir sinensis |
| title_sort | transcriptome analysis reveals potential regulatory mechanism of genes and pathways following aeromonas veronii infection and hypoxic stress in chinese mitten crab eriocheir sinensis |
| topic | Chinese mitten crab Hypoxic stress Aeromonas veronii infection Immunity Metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006951 |
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