Laboratory Identification of Metallo-beta-lactamase Producing Clinical Isolates of Pseudomonas aeruginosa: An Assessment of Different Phenotypic Methods
Background: Multidrug Resistant (MDR) Pseudomonas aeruginosa, an emerging superbug causing a wide spectrum of nosocomial infections. Carbapenem resistance among P. aeruginosa isolates is of grave concern and is mainly due to production of Metallobeta- lactamase (MBL) enzymes. Aim and Objectives...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
Krishna Vishwa Vidyapeeth (Deemed to be University), Karad
2018-07-01
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| Series: | Journal of Krishna Institute of Medical Sciences University |
| Subjects: | |
| Online Access: | http://www.jkimsu.com/jkimsu-vol7no3/JKIMSU,%20Vol.%207,%20No.%203,%20July-September%202018%20Page%2027-34.pdf |
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| Summary: | Background: Multidrug Resistant (MDR) Pseudomonas
aeruginosa, an emerging superbug causing a wide
spectrum of nosocomial infections. Carbapenem
resistance among P. aeruginosa isolates is of grave
concern and is mainly due to production of Metallobeta-
lactamase (MBL) enzymes. Aim and Objectives:
To find out the prevalence of MBL producing P.
aeruginosa isolates from various clinical samples and
to evaluate the efficacy of different phenotypic tests
employed in vitro for their detection. Material and
Methods:Atotal of 358 P. aeruginosa strains obtained
from different clinical samples were subjected to
antimicrobial susceptibility testing by Kirby-Bauer's
Disc Diffusion method. All the imipenem resistant
isolates were further tested by Modified Hodge Test
(MHT) to detect carbapenem resistance. MBL
production was tested by screening with Combined
Disc Test (CDT) and Double Disc Synergy Test
(DDST). MBL production was confirmed by MBL Etest
(Ab BioDisk). Results: Among 358 strains of P.
aeruginosa recovered, 114 isolates showed resistance
to imipenem. E test demonstrated 73 out of 114 isolates
as MBL producers. Of MHT, CDT and DDST tests
performed, DDST showed high sensitivity and
specificity. Conclusion: DDST can be suggested as an
economical option in place of expensive E test for
routine screening of MDR P. aeruginosa isolates for
MBL production in a clinical laboratory; which is
crucial for planning better management protocols. |
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| ISSN: | 2231-4261 2231-4261 |