Identification of potential geosmin-binding proteins in grass carp gill based on affinity responsive target stability and tandem mass tag proteomics
The escalating issue of water pollution, especially the accumulation of organic off-flavor pollutants, poses significant challenges. Geosmin, a typical off-flavor compound in aquatic environments, not only compromises the quality of aquatic products but also deters consumers. Its impact extends to a...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-02-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S014765132500168X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832540451456942080 |
|---|---|
| author | Jun-ming Zhang Huan Han Bing Fu Yi-chao Li Kang Li Jing-wei Liu Er-meng Yu Li-ping Liu |
| author_facet | Jun-ming Zhang Huan Han Bing Fu Yi-chao Li Kang Li Jing-wei Liu Er-meng Yu Li-ping Liu |
| author_sort | Jun-ming Zhang |
| collection | DOAJ |
| description | The escalating issue of water pollution, especially the accumulation of organic off-flavor pollutants, poses significant challenges. Geosmin, a typical off-flavor compound in aquatic environments, not only compromises the quality of aquatic products but also deters consumers. Its impact extends to aquatic organisms, with current research focusing on dose-response and ecotoxicity, while neglecting the molecular-level study of geosmin-binding proteins. This study employs an integrated approach combing affinity-responsive target stability in vitro, tandem mass tag proteomics in vivo, and molecular docking to identify geosmin-binding proteins in the gill tissue of grass carp (Ctenopharyngodon idella). ARTS analysis identified 56 proteins, predominantly membrane-associated proteins, such as catenin beta-1, annexin, and integrin beta. Proteomic analysis revealed 256 differentially expressed proteins in geosmin-exposure group, with 18 common proteins screened by in vivo and in vitro methods. Among these, annexin, cathepsin D, and interleukin-1 receptors were highlighted as potential geosmin targets, with annexin demonstrating the highest binding affinity in silico. This study provides a robust protocol integrating in vivo, in vitro, and in silico approaches to elucidate geosmin's target proteins in grass carp gill tissue, advancing our understanding of pollutant-biological interactions and enhancing environmental risk assessment accuracy. |
| format | Article |
| id | doaj-art-4395acaf5137412e9bd2758ddc24d70a |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-4395acaf5137412e9bd2758ddc24d70a2025-02-05T04:31:03ZengElsevierEcotoxicology and Environmental Safety0147-65132025-02-01291117832Identification of potential geosmin-binding proteins in grass carp gill based on affinity responsive target stability and tandem mass tag proteomicsJun-ming Zhang0Huan Han1Bing Fu2Yi-chao Li3Kang Li4Jing-wei Liu5Er-meng Yu6Li-ping Liu7China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Shanghai), Shanghai Ocean University, Shanghai 201306, ChinaChina-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Shanghai), Shanghai Ocean University, Shanghai 201306, ChinaCollege of Marine Sciences, South China Agricultural University, Guangzhou 510640, ChinaGuangxi Academy of Marine Sciences, Nanning, 530000, ChinaChina-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Shanghai), Shanghai Ocean University, Shanghai 201306, China; Center for Ecological Aquaculture (CEA), Shanghai Ocean University, Shanghai 201306, China; Corresponding authors at: China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Shanghai), Shanghai Ocean University, Shanghai 201306, China.China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Shanghai), Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, ChinaGuangxi Academy of Marine Sciences, Nanning, 530000, ChinaChina-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Shanghai), Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China; Corresponding authors at: China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Shanghai), Shanghai Ocean University, Shanghai 201306, China.The escalating issue of water pollution, especially the accumulation of organic off-flavor pollutants, poses significant challenges. Geosmin, a typical off-flavor compound in aquatic environments, not only compromises the quality of aquatic products but also deters consumers. Its impact extends to aquatic organisms, with current research focusing on dose-response and ecotoxicity, while neglecting the molecular-level study of geosmin-binding proteins. This study employs an integrated approach combing affinity-responsive target stability in vitro, tandem mass tag proteomics in vivo, and molecular docking to identify geosmin-binding proteins in the gill tissue of grass carp (Ctenopharyngodon idella). ARTS analysis identified 56 proteins, predominantly membrane-associated proteins, such as catenin beta-1, annexin, and integrin beta. Proteomic analysis revealed 256 differentially expressed proteins in geosmin-exposure group, with 18 common proteins screened by in vivo and in vitro methods. Among these, annexin, cathepsin D, and interleukin-1 receptors were highlighted as potential geosmin targets, with annexin demonstrating the highest binding affinity in silico. This study provides a robust protocol integrating in vivo, in vitro, and in silico approaches to elucidate geosmin's target proteins in grass carp gill tissue, advancing our understanding of pollutant-biological interactions and enhancing environmental risk assessment accuracy.http://www.sciencedirect.com/science/article/pii/S014765132500168XOff-flavor compoundsPollutant-protein interactionsAffinityBinding proteinMolecular dockingOrganic pollutants |
| spellingShingle | Jun-ming Zhang Huan Han Bing Fu Yi-chao Li Kang Li Jing-wei Liu Er-meng Yu Li-ping Liu Identification of potential geosmin-binding proteins in grass carp gill based on affinity responsive target stability and tandem mass tag proteomics Ecotoxicology and Environmental Safety Off-flavor compounds Pollutant-protein interactions Affinity Binding protein Molecular docking Organic pollutants |
| title | Identification of potential geosmin-binding proteins in grass carp gill based on affinity responsive target stability and tandem mass tag proteomics |
| title_full | Identification of potential geosmin-binding proteins in grass carp gill based on affinity responsive target stability and tandem mass tag proteomics |
| title_fullStr | Identification of potential geosmin-binding proteins in grass carp gill based on affinity responsive target stability and tandem mass tag proteomics |
| title_full_unstemmed | Identification of potential geosmin-binding proteins in grass carp gill based on affinity responsive target stability and tandem mass tag proteomics |
| title_short | Identification of potential geosmin-binding proteins in grass carp gill based on affinity responsive target stability and tandem mass tag proteomics |
| title_sort | identification of potential geosmin binding proteins in grass carp gill based on affinity responsive target stability and tandem mass tag proteomics |
| topic | Off-flavor compounds Pollutant-protein interactions Affinity Binding protein Molecular docking Organic pollutants |
| url | http://www.sciencedirect.com/science/article/pii/S014765132500168X |
| work_keys_str_mv | AT junmingzhang identificationofpotentialgeosminbindingproteinsingrasscarpgillbasedonaffinityresponsivetargetstabilityandtandemmasstagproteomics AT huanhan identificationofpotentialgeosminbindingproteinsingrasscarpgillbasedonaffinityresponsivetargetstabilityandtandemmasstagproteomics AT bingfu identificationofpotentialgeosminbindingproteinsingrasscarpgillbasedonaffinityresponsivetargetstabilityandtandemmasstagproteomics AT yichaoli identificationofpotentialgeosminbindingproteinsingrasscarpgillbasedonaffinityresponsivetargetstabilityandtandemmasstagproteomics AT kangli identificationofpotentialgeosminbindingproteinsingrasscarpgillbasedonaffinityresponsivetargetstabilityandtandemmasstagproteomics AT jingweiliu identificationofpotentialgeosminbindingproteinsingrasscarpgillbasedonaffinityresponsivetargetstabilityandtandemmasstagproteomics AT ermengyu identificationofpotentialgeosminbindingproteinsingrasscarpgillbasedonaffinityresponsivetargetstabilityandtandemmasstagproteomics AT lipingliu identificationofpotentialgeosminbindingproteinsingrasscarpgillbasedonaffinityresponsivetargetstabilityandtandemmasstagproteomics |