Combined extracts of Syzygium aromaticum (Clove) and Xylopia aethiopica (Negro pepper) seeds inhibit testosterone propionate-induced benign prostatic hyperplasia in Wistar rats
Objective: Study aimed to determine the inhibition of benign prostate hyperplasia (BPH) in rats using combined extracts of Syzygium aromaticum and Xylopia aethiopica. Methods: Twenty male Wistar rats were divided into four groups:(i) normal control (NC) received normal saline (0.5 mL); (ii) testoste...
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Taylor & Francis Group
2024-12-01
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| Online Access: | http://dx.doi.org/10.1080/26895293.2024.2435277 |
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| author | Emmanuel Nnaemeka Uhuo Simeon Ikechukwu Egba Chiemeziem Adnma Obike Paul Ndubuisi Anyiam Prince Ogochukwu Alaebo Pryanka Munachiso Okeke Emmanuella Chinaza Obinali |
| author_facet | Emmanuel Nnaemeka Uhuo Simeon Ikechukwu Egba Chiemeziem Adnma Obike Paul Ndubuisi Anyiam Prince Ogochukwu Alaebo Pryanka Munachiso Okeke Emmanuella Chinaza Obinali |
| author_sort | Emmanuel Nnaemeka Uhuo |
| collection | DOAJ |
| description | Objective: Study aimed to determine the inhibition of benign prostate hyperplasia (BPH) in rats using combined extracts of Syzygium aromaticum and Xylopia aethiopica. Methods: Twenty male Wistar rats were divided into four groups:(i) normal control (NC) received normal saline (0.5 mL); (ii) testosterone propionate (TP); (iii) and (iv)testosterone propionate + 200 and 400 mg/kg b.wt of the extract. Injection was performed subcutaneously, followed by daily oral administration of the extract for 30 days. Results: Prostate weight, testosterone, prostate specific antigen (PSA), and total protein levels increased significantly (p < 0.05) in the TP only compared with those in the NC and decreased in the treatment groups. Cholesterol, TAG, and LDL-chol increased in TP relative to TP-200 and TP-400. HDL increased non-significantly (p > 0.05) across the test groups compared to TP. MDA levels increased significantly (p < 0.05) in the TP compared with those in the test groups. Catalase and glutathione peroxidase (GPx) activities decreased significantly (p < 0.05) in the TP group compared to those in the NC, TP-200, and TP-400 groups. Similarly, superoxide dismutase (SOD) increased inTP-400 compared to that in the TP and NC groups. Decreased PCV %, Hb, RBC and platelets, except WBC, were recorded in TP compared to TP-200 and TP-400. TP-200 and TP-400 presented relatively normal prostatic histomorphology compared with TP. Conclusion: The mechanisms of BPH inhibition involve the regulation of sex hormone levels and the amelioration of conditions caused by oxidative damage. Abbreviations: BPH: benign prostatic hyperplasia; CAT: catalase; DTH: dihydrotestosterone; GPx: glutathione peroxidase; HDL-Chol: high-density lipoprotein cholesterol; LDL-C: low-density lipoprotein cholesterol; MDA: malondialdehyde; NC: normal control; PI: prostatic interstitium; PVC: packed cell volume; PSA: prostate specific antigen; SOD: superoxide dismutase; TP: testosterone propionate; TAG: triacylglycerol |
| format | Article |
| id | doaj-art-438959d47747456ea89ac81a6a620f65 |
| institution | Kabale University |
| issn | 2689-5307 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | All Life |
| spelling | doaj-art-438959d47747456ea89ac81a6a620f652025-01-20T14:38:00ZengTaylor & Francis GroupAll Life2689-53072024-12-01170110.1080/26895293.2024.24352772435277Combined extracts of Syzygium aromaticum (Clove) and Xylopia aethiopica (Negro pepper) seeds inhibit testosterone propionate-induced benign prostatic hyperplasia in Wistar ratsEmmanuel Nnaemeka Uhuo0Simeon Ikechukwu Egba1Chiemeziem Adnma Obike2Paul Ndubuisi Anyiam3Prince Ogochukwu Alaebo4Pryanka Munachiso Okeke5Emmanuella Chinaza Obinali6Michael Okpara University of AgricultureMichael Okpara University of AgricultureMichael Okpara University of AgricultureMichael Okpara University of AgricultureMichael Okpara University of AgricultureGodfrey Okoye UniversityGodfrey Okoye UniversityObjective: Study aimed to determine the inhibition of benign prostate hyperplasia (BPH) in rats using combined extracts of Syzygium aromaticum and Xylopia aethiopica. Methods: Twenty male Wistar rats were divided into four groups:(i) normal control (NC) received normal saline (0.5 mL); (ii) testosterone propionate (TP); (iii) and (iv)testosterone propionate + 200 and 400 mg/kg b.wt of the extract. Injection was performed subcutaneously, followed by daily oral administration of the extract for 30 days. Results: Prostate weight, testosterone, prostate specific antigen (PSA), and total protein levels increased significantly (p < 0.05) in the TP only compared with those in the NC and decreased in the treatment groups. Cholesterol, TAG, and LDL-chol increased in TP relative to TP-200 and TP-400. HDL increased non-significantly (p > 0.05) across the test groups compared to TP. MDA levels increased significantly (p < 0.05) in the TP compared with those in the test groups. Catalase and glutathione peroxidase (GPx) activities decreased significantly (p < 0.05) in the TP group compared to those in the NC, TP-200, and TP-400 groups. Similarly, superoxide dismutase (SOD) increased inTP-400 compared to that in the TP and NC groups. Decreased PCV %, Hb, RBC and platelets, except WBC, were recorded in TP compared to TP-200 and TP-400. TP-200 and TP-400 presented relatively normal prostatic histomorphology compared with TP. Conclusion: The mechanisms of BPH inhibition involve the regulation of sex hormone levels and the amelioration of conditions caused by oxidative damage. Abbreviations: BPH: benign prostatic hyperplasia; CAT: catalase; DTH: dihydrotestosterone; GPx: glutathione peroxidase; HDL-Chol: high-density lipoprotein cholesterol; LDL-C: low-density lipoprotein cholesterol; MDA: malondialdehyde; NC: normal control; PI: prostatic interstitium; PVC: packed cell volume; PSA: prostate specific antigen; SOD: superoxide dismutase; TP: testosterone propionate; TAG: triacylglycerolhttp://dx.doi.org/10.1080/26895293.2024.2435277prostateinhibitioncombined extractstestosterone propionate |
| spellingShingle | Emmanuel Nnaemeka Uhuo Simeon Ikechukwu Egba Chiemeziem Adnma Obike Paul Ndubuisi Anyiam Prince Ogochukwu Alaebo Pryanka Munachiso Okeke Emmanuella Chinaza Obinali Combined extracts of Syzygium aromaticum (Clove) and Xylopia aethiopica (Negro pepper) seeds inhibit testosterone propionate-induced benign prostatic hyperplasia in Wistar rats All Life prostate inhibition combined extracts testosterone propionate |
| title | Combined extracts of Syzygium aromaticum (Clove) and Xylopia aethiopica (Negro pepper) seeds inhibit testosterone propionate-induced benign prostatic hyperplasia in Wistar rats |
| title_full | Combined extracts of Syzygium aromaticum (Clove) and Xylopia aethiopica (Negro pepper) seeds inhibit testosterone propionate-induced benign prostatic hyperplasia in Wistar rats |
| title_fullStr | Combined extracts of Syzygium aromaticum (Clove) and Xylopia aethiopica (Negro pepper) seeds inhibit testosterone propionate-induced benign prostatic hyperplasia in Wistar rats |
| title_full_unstemmed | Combined extracts of Syzygium aromaticum (Clove) and Xylopia aethiopica (Negro pepper) seeds inhibit testosterone propionate-induced benign prostatic hyperplasia in Wistar rats |
| title_short | Combined extracts of Syzygium aromaticum (Clove) and Xylopia aethiopica (Negro pepper) seeds inhibit testosterone propionate-induced benign prostatic hyperplasia in Wistar rats |
| title_sort | combined extracts of syzygium aromaticum clove and xylopia aethiopica negro pepper seeds inhibit testosterone propionate induced benign prostatic hyperplasia in wistar rats |
| topic | prostate inhibition combined extracts testosterone propionate |
| url | http://dx.doi.org/10.1080/26895293.2024.2435277 |
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