EOLA1 Inhibits Lipopolysaccharide-Induced Vascular Cell Adhesion Molecule-1 Expression by Association with MT2A in ECV304 Cells
Our research group firstly discovered endothelial-overexpressed lipopolysaccharide-associated factor 1 (EOLA1, GenBank number AY074889) as a lipopolysaccharide (LPS) responsive gene in ECV304 cells. The previous studies have further demonstrated the association of EOLA1 with metallothionein 2A (MT2A...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/301562 |
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| _version_ | 1832559271036846080 |
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| author | Weiling Leng Xiaotian Lei Hao Meng Xinshou Ouyang Ziwen Liang |
| author_facet | Weiling Leng Xiaotian Lei Hao Meng Xinshou Ouyang Ziwen Liang |
| author_sort | Weiling Leng |
| collection | DOAJ |
| description | Our research group firstly discovered endothelial-overexpressed lipopolysaccharide-associated factor 1 (EOLA1, GenBank number AY074889) as a lipopolysaccharide (LPS) responsive gene in ECV304 cells. The previous studies have further demonstrated the association of EOLA1 with metallothionein 2A (MT2A), while the role of EOLA1 during LPS-induced inflammatory response in ECV304 cells is unknown. In this report, we determined the subcellular localization of EOLA1 and the regulatory capacity of EOLA1 on vascular cell adhesion molecule-1 (VCAM-1) in response to LPS in ECV304 cells. Our results show that EOLA1 is broadly diffuse in the cells, and EOLA1 expression is dramatically induced by LPS. EOLA1 knockdown results in significant enhancement of LPS-induced VCAM-1 production. Consistent with this, overexpression of EOLA1 leads to the reduction of LPS-induced VCAM-1 production. Furthermore, MT2A knockdown reduces LPS-induced VCAM-1 production. Collectively, our results demonstrate a negative regulatory role of EOLA1 on LPS-induced VCAM-1 expression involving its association with MT2A in ECV304 cells. |
| format | Article |
| id | doaj-art-437ed50064fd40b684a2314a2fc51f53 |
| institution | Kabale University |
| issn | 2090-8040 2042-0099 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Inflammation |
| spelling | doaj-art-437ed50064fd40b684a2314a2fc51f532025-02-03T01:30:27ZengWileyInternational Journal of Inflammation2090-80402042-00992015-01-01201510.1155/2015/301562301562EOLA1 Inhibits Lipopolysaccharide-Induced Vascular Cell Adhesion Molecule-1 Expression by Association with MT2A in ECV304 CellsWeiling Leng0Xiaotian Lei1Hao Meng2Xinshou Ouyang3Ziwen Liang4Department of Endocrinology, Southwest Hospital, Third Military Medical University, Chongqing 400038, ChinaDepartment of Endocrinology, Southwest Hospital, Third Military Medical University, Chongqing 400038, ChinaDepartment of Endocrinology, Southwest Hospital, Third Military Medical University, Chongqing 400038, ChinaSection of Digestive Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06519, USADepartment of Endocrinology, Southwest Hospital, Third Military Medical University, Chongqing 400038, ChinaOur research group firstly discovered endothelial-overexpressed lipopolysaccharide-associated factor 1 (EOLA1, GenBank number AY074889) as a lipopolysaccharide (LPS) responsive gene in ECV304 cells. The previous studies have further demonstrated the association of EOLA1 with metallothionein 2A (MT2A), while the role of EOLA1 during LPS-induced inflammatory response in ECV304 cells is unknown. In this report, we determined the subcellular localization of EOLA1 and the regulatory capacity of EOLA1 on vascular cell adhesion molecule-1 (VCAM-1) in response to LPS in ECV304 cells. Our results show that EOLA1 is broadly diffuse in the cells, and EOLA1 expression is dramatically induced by LPS. EOLA1 knockdown results in significant enhancement of LPS-induced VCAM-1 production. Consistent with this, overexpression of EOLA1 leads to the reduction of LPS-induced VCAM-1 production. Furthermore, MT2A knockdown reduces LPS-induced VCAM-1 production. Collectively, our results demonstrate a negative regulatory role of EOLA1 on LPS-induced VCAM-1 expression involving its association with MT2A in ECV304 cells.http://dx.doi.org/10.1155/2015/301562 |
| spellingShingle | Weiling Leng Xiaotian Lei Hao Meng Xinshou Ouyang Ziwen Liang EOLA1 Inhibits Lipopolysaccharide-Induced Vascular Cell Adhesion Molecule-1 Expression by Association with MT2A in ECV304 Cells International Journal of Inflammation |
| title | EOLA1 Inhibits Lipopolysaccharide-Induced Vascular Cell Adhesion Molecule-1 Expression by Association with MT2A in ECV304 Cells |
| title_full | EOLA1 Inhibits Lipopolysaccharide-Induced Vascular Cell Adhesion Molecule-1 Expression by Association with MT2A in ECV304 Cells |
| title_fullStr | EOLA1 Inhibits Lipopolysaccharide-Induced Vascular Cell Adhesion Molecule-1 Expression by Association with MT2A in ECV304 Cells |
| title_full_unstemmed | EOLA1 Inhibits Lipopolysaccharide-Induced Vascular Cell Adhesion Molecule-1 Expression by Association with MT2A in ECV304 Cells |
| title_short | EOLA1 Inhibits Lipopolysaccharide-Induced Vascular Cell Adhesion Molecule-1 Expression by Association with MT2A in ECV304 Cells |
| title_sort | eola1 inhibits lipopolysaccharide induced vascular cell adhesion molecule 1 expression by association with mt2a in ecv304 cells |
| url | http://dx.doi.org/10.1155/2015/301562 |
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