A chromosome-level genome assembly of the cabbage aphid Brevicoryne brassicae
Abstract The cabbage aphid, Brevicoryne brassicae, is a major pest on Brassicaceae plants, causing significant yield losses annually. However, the lack of genomic resources has hindered progress in understanding this pest at the molecular level. Here, we present a high-quality, chromosomal-level gen...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-04501-2 |
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| author | Jun Wu Guomeng Li Zhimou Lin Yangzhi Zhang Wenyuan Yu Rong Hu Shuai Zhan Yazhou Chen |
| author_facet | Jun Wu Guomeng Li Zhimou Lin Yangzhi Zhang Wenyuan Yu Rong Hu Shuai Zhan Yazhou Chen |
| author_sort | Jun Wu |
| collection | DOAJ |
| description | Abstract The cabbage aphid, Brevicoryne brassicae, is a major pest on Brassicaceae plants, causing significant yield losses annually. However, the lack of genomic resources has hindered progress in understanding this pest at the molecular level. Here, we present a high-quality, chromosomal-level genome assembly for B. brassicae, based on PacBio HiFi long-read sequencing and Hi-C data. The final assembled genome size was 429.99 Mb, with a scaffold N50 of 93.31 Mb. Notably, 96.19% of the assembled sequences were anchored to eight chromosomes. The genome covered 99.24% of BUSCO genes and 95.16% of CEGMA genes, indicating a high level of completeness. By integrating high-coverage transcriptome data, we annotated 22,671 protein-coding genes and 3,594 lncRNA genes. Preliminary comparative genomic analyses focused on genes related to host colonization, such as chemosensory- and detoxification-related genes, as well as cross-kingdom lncRNA Ya. In summary, this study presents a contiguous and complete genome for B. brassicae, which will advance our understanding of the molecular mechanisms underlying its host adaptation, pest behavior, and interaction with Brassicaceae plants. |
| format | Article |
| id | doaj-art-4354e33ac4d649d09e64bf1c046f87bf |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Data |
| spelling | doaj-art-4354e33ac4d649d09e64bf1c046f87bf2025-02-02T12:08:03ZengNature PortfolioScientific Data2052-44632025-01-011211810.1038/s41597-025-04501-2A chromosome-level genome assembly of the cabbage aphid Brevicoryne brassicaeJun Wu0Guomeng Li1Zhimou Lin2Yangzhi Zhang3Wenyuan Yu4Rong Hu5Shuai Zhan6Yazhou Chen7Hubei Hongshan LaboratoryHubei Hongshan LaboratoryHubei Hongshan LaboratoryHubei Hongshan LaboratoryHubei Hongshan LaboratoryHubei Hongshan LaboratoryCAS Key Laboratory of Insect Developmental and Evolutionary Biology, Center for Excellence in Molecular Plant Sciences, Chinese Academy of SciencesHubei Hongshan LaboratoryAbstract The cabbage aphid, Brevicoryne brassicae, is a major pest on Brassicaceae plants, causing significant yield losses annually. However, the lack of genomic resources has hindered progress in understanding this pest at the molecular level. Here, we present a high-quality, chromosomal-level genome assembly for B. brassicae, based on PacBio HiFi long-read sequencing and Hi-C data. The final assembled genome size was 429.99 Mb, with a scaffold N50 of 93.31 Mb. Notably, 96.19% of the assembled sequences were anchored to eight chromosomes. The genome covered 99.24% of BUSCO genes and 95.16% of CEGMA genes, indicating a high level of completeness. By integrating high-coverage transcriptome data, we annotated 22,671 protein-coding genes and 3,594 lncRNA genes. Preliminary comparative genomic analyses focused on genes related to host colonization, such as chemosensory- and detoxification-related genes, as well as cross-kingdom lncRNA Ya. In summary, this study presents a contiguous and complete genome for B. brassicae, which will advance our understanding of the molecular mechanisms underlying its host adaptation, pest behavior, and interaction with Brassicaceae plants.https://doi.org/10.1038/s41597-025-04501-2 |
| spellingShingle | Jun Wu Guomeng Li Zhimou Lin Yangzhi Zhang Wenyuan Yu Rong Hu Shuai Zhan Yazhou Chen A chromosome-level genome assembly of the cabbage aphid Brevicoryne brassicae Scientific Data |
| title | A chromosome-level genome assembly of the cabbage aphid Brevicoryne brassicae |
| title_full | A chromosome-level genome assembly of the cabbage aphid Brevicoryne brassicae |
| title_fullStr | A chromosome-level genome assembly of the cabbage aphid Brevicoryne brassicae |
| title_full_unstemmed | A chromosome-level genome assembly of the cabbage aphid Brevicoryne brassicae |
| title_short | A chromosome-level genome assembly of the cabbage aphid Brevicoryne brassicae |
| title_sort | chromosome level genome assembly of the cabbage aphid brevicoryne brassicae |
| url | https://doi.org/10.1038/s41597-025-04501-2 |
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