Protective Effect of Nanoparticles Loaded with Black Rice Anthocyanins on Caco-2 Cell Damage
In this study, bovine serum albumin (BSA) and genipin (GP) were used to prepare BSA nanoparticles loaded with black rice anthocyanins (BRA-BSAGP) to improve the stability and bioactivity of BRA. The interaction between BSAGP and BRA was analyzed, the release characteristics of BRA-BSAGP during simul...
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China Food Publishing Company
2025-02-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-3-012.pdf |
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| author | LIU Yaqi, ZHOU Na, FENG Mengmeng, AI Xin, ZHAO Lei, ZHAO Liang |
| author_facet | LIU Yaqi, ZHOU Na, FENG Mengmeng, AI Xin, ZHAO Lei, ZHAO Liang |
| author_sort | LIU Yaqi, ZHOU Na, FENG Mengmeng, AI Xin, ZHAO Lei, ZHAO Liang |
| collection | DOAJ |
| description | In this study, bovine serum albumin (BSA) and genipin (GP) were used to prepare BSA nanoparticles loaded with black rice anthocyanins (BRA-BSAGP) to improve the stability and bioactivity of BRA. The interaction between BSAGP and BRA was analyzed, the release characteristics of BRA-BSAGP during simulated gastrointestinal digestion were explored, and the ameliorative effect and mechanism of BRA-BSAGP on Caco-2 cell damage induced by dextran sulfate sodium (DSS) were investigated. BRA-BSAGP showed a uniform spherical morphology with an encapsulation efficiency of (81.10 ± 0.08)%. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis confirmed the successful encapsulation of BRA in BSA nanoparticles. In vitro gastrointestinal digestion demonstrated that BRA-BSAGP nanoparticles effectively delayed the release of BRA, with release percentages of 22.51% and 20.10% under simulated fasting and fed conditions, respectively. BRA-BSAGP significantly increased the survival rate of Caco-2 cells induced by DSS and up-regulated the expression of tight junction proteins (Claudin-1, occludin, and ZO-1). This study provides a theoretical basis for the application of BRA in developing functional foods for alleviating ulcerative colitis. |
| format | Article |
| id | doaj-art-434d2b4fe99d457196cb46a743b2f2d1 |
| institution | Kabale University |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-434d2b4fe99d457196cb46a743b2f2d12025-02-05T09:08:33ZengChina Food Publishing CompanyShipin Kexue1002-66302025-02-0146310210910.7506/spkx1002-6630-20240703-032Protective Effect of Nanoparticles Loaded with Black Rice Anthocyanins on Caco-2 Cell DamageLIU Yaqi, ZHOU Na, FENG Mengmeng, AI Xin, ZHAO Lei, ZHAO Liang0(1. Key Laboratory of Geriatric Nutrition and Health, Ministry of Education, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China; 2. Heze Vocational College, Heze 274002, China)In this study, bovine serum albumin (BSA) and genipin (GP) were used to prepare BSA nanoparticles loaded with black rice anthocyanins (BRA-BSAGP) to improve the stability and bioactivity of BRA. The interaction between BSAGP and BRA was analyzed, the release characteristics of BRA-BSAGP during simulated gastrointestinal digestion were explored, and the ameliorative effect and mechanism of BRA-BSAGP on Caco-2 cell damage induced by dextran sulfate sodium (DSS) were investigated. BRA-BSAGP showed a uniform spherical morphology with an encapsulation efficiency of (81.10 ± 0.08)%. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis confirmed the successful encapsulation of BRA in BSA nanoparticles. In vitro gastrointestinal digestion demonstrated that BRA-BSAGP nanoparticles effectively delayed the release of BRA, with release percentages of 22.51% and 20.10% under simulated fasting and fed conditions, respectively. BRA-BSAGP significantly increased the survival rate of Caco-2 cells induced by DSS and up-regulated the expression of tight junction proteins (Claudin-1, occludin, and ZO-1). This study provides a theoretical basis for the application of BRA in developing functional foods for alleviating ulcerative colitis.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-3-012.pdfblack rice anthocyanins; bovine serum albumin; genipin; caco-2 cell |
| spellingShingle | LIU Yaqi, ZHOU Na, FENG Mengmeng, AI Xin, ZHAO Lei, ZHAO Liang Protective Effect of Nanoparticles Loaded with Black Rice Anthocyanins on Caco-2 Cell Damage Shipin Kexue black rice anthocyanins; bovine serum albumin; genipin; caco-2 cell |
| title | Protective Effect of Nanoparticles Loaded with Black Rice Anthocyanins on Caco-2 Cell Damage |
| title_full | Protective Effect of Nanoparticles Loaded with Black Rice Anthocyanins on Caco-2 Cell Damage |
| title_fullStr | Protective Effect of Nanoparticles Loaded with Black Rice Anthocyanins on Caco-2 Cell Damage |
| title_full_unstemmed | Protective Effect of Nanoparticles Loaded with Black Rice Anthocyanins on Caco-2 Cell Damage |
| title_short | Protective Effect of Nanoparticles Loaded with Black Rice Anthocyanins on Caco-2 Cell Damage |
| title_sort | protective effect of nanoparticles loaded with black rice anthocyanins on caco 2 cell damage |
| topic | black rice anthocyanins; bovine serum albumin; genipin; caco-2 cell |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-3-012.pdf |
| work_keys_str_mv | AT liuyaqizhounafengmengmengaixinzhaoleizhaoliang protectiveeffectofnanoparticlesloadedwithblackriceanthocyaninsoncaco2celldamage |