Protective Effect of Nanoparticles Loaded with Black Rice Anthocyanins on Caco-2 Cell Damage
In this study, bovine serum albumin (BSA) and genipin (GP) were used to prepare BSA nanoparticles loaded with black rice anthocyanins (BRA-BSAGP) to improve the stability and bioactivity of BRA. The interaction between BSAGP and BRA was analyzed, the release characteristics of BRA-BSAGP during simul...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
China Food Publishing Company
2025-02-01
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| Series: | Shipin Kexue |
| Subjects: | |
| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-3-012.pdf |
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| Summary: | In this study, bovine serum albumin (BSA) and genipin (GP) were used to prepare BSA nanoparticles loaded with black rice anthocyanins (BRA-BSAGP) to improve the stability and bioactivity of BRA. The interaction between BSAGP and BRA was analyzed, the release characteristics of BRA-BSAGP during simulated gastrointestinal digestion were explored, and the ameliorative effect and mechanism of BRA-BSAGP on Caco-2 cell damage induced by dextran sulfate sodium (DSS) were investigated. BRA-BSAGP showed a uniform spherical morphology with an encapsulation efficiency of (81.10 ± 0.08)%. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis confirmed the successful encapsulation of BRA in BSA nanoparticles. In vitro gastrointestinal digestion demonstrated that BRA-BSAGP nanoparticles effectively delayed the release of BRA, with release percentages of 22.51% and 20.10% under simulated fasting and fed conditions, respectively. BRA-BSAGP significantly increased the survival rate of Caco-2 cells induced by DSS and up-regulated the expression of tight junction proteins (Claudin-1, occludin, and ZO-1). This study provides a theoretical basis for the application of BRA in developing functional foods for alleviating ulcerative colitis. |
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| ISSN: | 1002-6630 |