Acetylation Modification Improves Immunoregulatory Effect of Polysaccharide from Seeds of Plantago asiatica L.
The current study was conducted to investigate effects of acetylated Plantago asiatica L. polysaccharides (Ac-PLCPs) on their immunoregulatory activities in bone-marrow-derived dendritic cells (DCs) model. Influences of natural Plantago asiatica L. crude polysaccharide (PLCP) and Ac-PLCPs on inducin...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/3082026 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556879805415424 |
|---|---|
| author | Le-Ming Jiang Shao-Ping Nie Dan-Fei Huang Zhi-Hong Fu Ming-Yong Xie |
| author_facet | Le-Ming Jiang Shao-Ping Nie Dan-Fei Huang Zhi-Hong Fu Ming-Yong Xie |
| author_sort | Le-Ming Jiang |
| collection | DOAJ |
| description | The current study was conducted to investigate effects of acetylated Plantago asiatica L. polysaccharides (Ac-PLCPs) on their immunoregulatory activities in bone-marrow-derived dendritic cells (DCs) model. Influences of natural Plantago asiatica L. crude polysaccharide (PLCP) and Ac-PLCPs on inducing phenotypic and functional maturation on DCs were determined. The results showed that Ac-PLCPs with degree of substitution (DS) from 0.06 to 0.1 could not only stimulate the expression of surface molecules major histocompatibility complex class II (MHC II), cluster of differentiation 86 (CD86), and CD80 on DCs (P<0.01) but also increase the secretion of cytokine IL-12p70 (P<0.01). The endocytosis activity of DCs was attenuated by Ac-PLCPs treatment (P<0.01), while the mRNA expressions of chemokine receptors CCR7 and CXCR4 in DCs were significantly increased (P<0.01). Besides, DCs treated with the Ac-PLCPs showed extremely strong T cell proliferation stimulating activity (P<0.01). These data showed that Ac-PLCPs had higher maturation-stimulating activity on DCs than PLCP, which indicated that acetylation modification improved the immunoregulatory effect of PLCP. |
| format | Article |
| id | doaj-art-434100dc738d4c63bf2f866a0a802d31 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-434100dc738d4c63bf2f866a0a802d312025-02-03T05:44:13ZengWileyJournal of Chemistry2090-90632090-90712018-01-01201810.1155/2018/30820263082026Acetylation Modification Improves Immunoregulatory Effect of Polysaccharide from Seeds of Plantago asiatica L.Le-Ming Jiang0Shao-Ping Nie1Dan-Fei Huang2Zhi-Hong Fu3Ming-Yong Xie4State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaThe current study was conducted to investigate effects of acetylated Plantago asiatica L. polysaccharides (Ac-PLCPs) on their immunoregulatory activities in bone-marrow-derived dendritic cells (DCs) model. Influences of natural Plantago asiatica L. crude polysaccharide (PLCP) and Ac-PLCPs on inducing phenotypic and functional maturation on DCs were determined. The results showed that Ac-PLCPs with degree of substitution (DS) from 0.06 to 0.1 could not only stimulate the expression of surface molecules major histocompatibility complex class II (MHC II), cluster of differentiation 86 (CD86), and CD80 on DCs (P<0.01) but also increase the secretion of cytokine IL-12p70 (P<0.01). The endocytosis activity of DCs was attenuated by Ac-PLCPs treatment (P<0.01), while the mRNA expressions of chemokine receptors CCR7 and CXCR4 in DCs were significantly increased (P<0.01). Besides, DCs treated with the Ac-PLCPs showed extremely strong T cell proliferation stimulating activity (P<0.01). These data showed that Ac-PLCPs had higher maturation-stimulating activity on DCs than PLCP, which indicated that acetylation modification improved the immunoregulatory effect of PLCP.http://dx.doi.org/10.1155/2018/3082026 |
| spellingShingle | Le-Ming Jiang Shao-Ping Nie Dan-Fei Huang Zhi-Hong Fu Ming-Yong Xie Acetylation Modification Improves Immunoregulatory Effect of Polysaccharide from Seeds of Plantago asiatica L. Journal of Chemistry |
| title | Acetylation Modification Improves Immunoregulatory Effect of Polysaccharide from Seeds of Plantago asiatica L. |
| title_full | Acetylation Modification Improves Immunoregulatory Effect of Polysaccharide from Seeds of Plantago asiatica L. |
| title_fullStr | Acetylation Modification Improves Immunoregulatory Effect of Polysaccharide from Seeds of Plantago asiatica L. |
| title_full_unstemmed | Acetylation Modification Improves Immunoregulatory Effect of Polysaccharide from Seeds of Plantago asiatica L. |
| title_short | Acetylation Modification Improves Immunoregulatory Effect of Polysaccharide from Seeds of Plantago asiatica L. |
| title_sort | acetylation modification improves immunoregulatory effect of polysaccharide from seeds of plantago asiatica l |
| url | http://dx.doi.org/10.1155/2018/3082026 |
| work_keys_str_mv | AT lemingjiang acetylationmodificationimprovesimmunoregulatoryeffectofpolysaccharidefromseedsofplantagoasiatical AT shaopingnie acetylationmodificationimprovesimmunoregulatoryeffectofpolysaccharidefromseedsofplantagoasiatical AT danfeihuang acetylationmodificationimprovesimmunoregulatoryeffectofpolysaccharidefromseedsofplantagoasiatical AT zhihongfu acetylationmodificationimprovesimmunoregulatoryeffectofpolysaccharidefromseedsofplantagoasiatical AT mingyongxie acetylationmodificationimprovesimmunoregulatoryeffectofpolysaccharidefromseedsofplantagoasiatical |