Effectiveness of predatory bacterium Bdellovibrio bacteriovorus in combination with Pseudomonas fluorescens and Lactobacillus acidophilus as candidates for in vitro anticolibacillosis
Background: Bdellovibrio bacteriovorus has stood out since its initial discovery because of its exceptional capacity to feed on other Gram-negative bacteria. Since this specific "predatory bacterium" may be used as both a probiotic and an antibiotic, research on it has expanded in...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Tripoli University
2025-03-01
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| Series: | Open Veterinary Journal |
| Subjects: | |
| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=234167 |
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| Summary: | Background:
Bdellovibrio bacteriovorus has stood out since its initial discovery because of its exceptional capacity to feed on other Gram-negative bacteria. Since this specific "predatory bacterium" may be used as both a probiotic and an antibiotic, research on it has expanded in response to the growing issue of AMR. It is necessary to investigate the relationship between B. bacteriovorus and other Gram-negative bacteria, as well as the presence of Gram-positive bacteria, in the same environment to determine whether or not these factors have an impact on their ability as predators.
Aim:
This study aimed to assess the effectiveness of the combination of the predatory bacterium B. bacteriovorus with Pseudomonas fluorescens and Lactobacillus acidophilus as potential candidates for in vitro anticolibacillosis.
Methods:
The method employed Escherichia coli ATCC 15144 as the prey, while B. bacteriovorus 109 J ATCC 15143 was used as the predator, combined with P. fluorescens and L. acidophilus as the nutrient sources of the predator. In the challenge experiment, a ratio of the bacterial combination was used to optimize predation to E. coli of 107 PFU/105 CFU per ml.
Results:
The study showed that the combination of B. bacteriovorus with P. fluorescens and L. acidophilus after 24 hours of in vitro incubation at 37°C increased the predatory bacteria count by tenfold, effectively reducing the E. coli population. However, in the absence of Gram-negative bacteria as a nutrient source, the predator population gradually declined.
Conclusion:
The combination of B. bacteriovorus as a predatory bacterium with P. fluorescens and L. acidophilus is an effective candidate for in vitro anticolibacillosis. [Open Vet J 2025; 15(3.000): 1379-1386] |
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| ISSN: | 2226-4485 2218-6050 |