Novel Injectable Collagen/Glycerol/Pullulan Gel Promotes Osteogenic Differentiation of Mesenchymal Stem Cells and the Repair of Rat Cranial Defects
Bone tissue engineering is a technique that simulates the bone tissue microenvironment by utilizing cells, tissue scaffolds, and growth factors. The collagen hydrogel is a three-dimensional network bionic material that has properties and structures comparable to those of the extracellular matrix (EC...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Gels |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2310-2861/10/12/775 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850049614422474752 |
|---|---|
| author | Xin Wang Satoshi Komasa Yoshiro Tahara Shihoko Inui Michiaki Matsumoto Kenji Maekawa |
| author_facet | Xin Wang Satoshi Komasa Yoshiro Tahara Shihoko Inui Michiaki Matsumoto Kenji Maekawa |
| author_sort | Xin Wang |
| collection | DOAJ |
| description | Bone tissue engineering is a technique that simulates the bone tissue microenvironment by utilizing cells, tissue scaffolds, and growth factors. The collagen hydrogel is a three-dimensional network bionic material that has properties and structures comparable to those of the extracellular matrix (ECM), making it an ideal scaffold and drug delivery system for tissue engineering. The clinical applications of this material are restricted due to its low mechanical strength. In this investigation, a collagen-based gel (atelocollagen/glycerol/pullulan [Col/Gly/Pul] gel) that is moldable and injectable with high adhesive qualities was created by employing a straightforward technique that involved the introduction of Gly and Pul. This study aimed to characterize the internal morphology and chemical composition of the Col/Gly/Pul gel, as well as to verify its osteogenic properties through in vivo and in vitro experiments. When compared to a standard pure Col hydrogel, this material is more adaptable to the complexity of the local environment of bone defects and the apposition of irregularly shaped flaws due to its greater mechanical strength, injectability, and moldability. Overall, the Col/Gly/Pul gel is an implant that shows great potential for the treatment of complex bone defects and the enhancement of bone regeneration. |
| format | Article |
| id | doaj-art-42d5eca0941e4db8a84c7b0cf00a9663 |
| institution | DOAJ |
| issn | 2310-2861 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj-art-42d5eca0941e4db8a84c7b0cf00a96632025-08-20T02:53:41ZengMDPI AGGels2310-28612024-11-01101277510.3390/gels10120775Novel Injectable Collagen/Glycerol/Pullulan Gel Promotes Osteogenic Differentiation of Mesenchymal Stem Cells and the Repair of Rat Cranial DefectsXin Wang0Satoshi Komasa1Yoshiro Tahara2Shihoko Inui3Michiaki Matsumoto4Kenji Maekawa5Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1, Kuzuhahanazono-cho, Hirakata-shi 573-1121, Osaka, JapanDepartment of Oral Health Sciences, Osaka Dental University, 1-4-4, Makino-honmachi, Hirakata-shi 573-1144, Osaka, JapanDepartment of Chemical Engineering and Materials Science, Doshisha University, 1-3 Tatara-Miyakodani, Kyotanabe 610-0321, Kyoto, JapanDepartment of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1, Kuzuhahanazono-cho, Hirakata-shi 573-1121, Osaka, JapanDepartment of Chemical Engineering and Materials Science, Doshisha University, 1-3 Tatara-Miyakodani, Kyotanabe 610-0321, Kyoto, JapanDepartment of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1, Kuzuhahanazono-cho, Hirakata-shi 573-1121, Osaka, JapanBone tissue engineering is a technique that simulates the bone tissue microenvironment by utilizing cells, tissue scaffolds, and growth factors. The collagen hydrogel is a three-dimensional network bionic material that has properties and structures comparable to those of the extracellular matrix (ECM), making it an ideal scaffold and drug delivery system for tissue engineering. The clinical applications of this material are restricted due to its low mechanical strength. In this investigation, a collagen-based gel (atelocollagen/glycerol/pullulan [Col/Gly/Pul] gel) that is moldable and injectable with high adhesive qualities was created by employing a straightforward technique that involved the introduction of Gly and Pul. This study aimed to characterize the internal morphology and chemical composition of the Col/Gly/Pul gel, as well as to verify its osteogenic properties through in vivo and in vitro experiments. When compared to a standard pure Col hydrogel, this material is more adaptable to the complexity of the local environment of bone defects and the apposition of irregularly shaped flaws due to its greater mechanical strength, injectability, and moldability. Overall, the Col/Gly/Pul gel is an implant that shows great potential for the treatment of complex bone defects and the enhancement of bone regeneration.https://www.mdpi.com/2310-2861/10/12/775collagenpullulaninjectable gelmechanical propertiesosteogenic differentiationbone repair |
| spellingShingle | Xin Wang Satoshi Komasa Yoshiro Tahara Shihoko Inui Michiaki Matsumoto Kenji Maekawa Novel Injectable Collagen/Glycerol/Pullulan Gel Promotes Osteogenic Differentiation of Mesenchymal Stem Cells and the Repair of Rat Cranial Defects Gels collagen pullulan injectable gel mechanical properties osteogenic differentiation bone repair |
| title | Novel Injectable Collagen/Glycerol/Pullulan Gel Promotes Osteogenic Differentiation of Mesenchymal Stem Cells and the Repair of Rat Cranial Defects |
| title_full | Novel Injectable Collagen/Glycerol/Pullulan Gel Promotes Osteogenic Differentiation of Mesenchymal Stem Cells and the Repair of Rat Cranial Defects |
| title_fullStr | Novel Injectable Collagen/Glycerol/Pullulan Gel Promotes Osteogenic Differentiation of Mesenchymal Stem Cells and the Repair of Rat Cranial Defects |
| title_full_unstemmed | Novel Injectable Collagen/Glycerol/Pullulan Gel Promotes Osteogenic Differentiation of Mesenchymal Stem Cells and the Repair of Rat Cranial Defects |
| title_short | Novel Injectable Collagen/Glycerol/Pullulan Gel Promotes Osteogenic Differentiation of Mesenchymal Stem Cells and the Repair of Rat Cranial Defects |
| title_sort | novel injectable collagen glycerol pullulan gel promotes osteogenic differentiation of mesenchymal stem cells and the repair of rat cranial defects |
| topic | collagen pullulan injectable gel mechanical properties osteogenic differentiation bone repair |
| url | https://www.mdpi.com/2310-2861/10/12/775 |
| work_keys_str_mv | AT xinwang novelinjectablecollagenglycerolpullulangelpromotesosteogenicdifferentiationofmesenchymalstemcellsandtherepairofratcranialdefects AT satoshikomasa novelinjectablecollagenglycerolpullulangelpromotesosteogenicdifferentiationofmesenchymalstemcellsandtherepairofratcranialdefects AT yoshirotahara novelinjectablecollagenglycerolpullulangelpromotesosteogenicdifferentiationofmesenchymalstemcellsandtherepairofratcranialdefects AT shihokoinui novelinjectablecollagenglycerolpullulangelpromotesosteogenicdifferentiationofmesenchymalstemcellsandtherepairofratcranialdefects AT michiakimatsumoto novelinjectablecollagenglycerolpullulangelpromotesosteogenicdifferentiationofmesenchymalstemcellsandtherepairofratcranialdefects AT kenjimaekawa novelinjectablecollagenglycerolpullulangelpromotesosteogenicdifferentiationofmesenchymalstemcellsandtherepairofratcranialdefects |