Modular cone-and-plate device for mechanofluidic assays in Transwell inserts
In this work, we present a cost effective and open-source modular cone-and-plate (MoCAP) device that incorporates shear stress in the popular Transwell® insert system. This system acts as a lid that incorporates flow into 24-well Transwell® inserts while preserving the ability to conduct molecular p...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1494553/full |
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| author | Daniel Chavarria Kissamy A. Georges Brian J. O’Grady Khalid K. Hassan Ethan S. Lippmann Ethan S. Lippmann Ethan S. Lippmann Ethan S. Lippmann Ethan S. Lippmann Ethan S. Lippmann |
| author_facet | Daniel Chavarria Kissamy A. Georges Brian J. O’Grady Khalid K. Hassan Ethan S. Lippmann Ethan S. Lippmann Ethan S. Lippmann Ethan S. Lippmann Ethan S. Lippmann Ethan S. Lippmann |
| author_sort | Daniel Chavarria |
| collection | DOAJ |
| description | In this work, we present a cost effective and open-source modular cone-and-plate (MoCAP) device that incorporates shear stress in the popular Transwell® insert system. This system acts as a lid that incorporates flow into 24-well Transwell® inserts while preserving the ability to conduct molecular profiling assays. Moreover, the MoCAP device can be rapidly reconfigured to test multiple shear stress profiles within a single device. To demonstrate the utility of the MoCAP, we conducted select assays on several different brain microvascular endothelial cell (BMEC) lines that comprise models of the blood-brain barrier (BBB), since shear stress can play an important role in BBB function. Our results characterize how shear stress modulates passive barrier function and GLUT1 expression across the different BMEC lines. Overall, we anticipate this low cost mechanofluidic device will be useful to the mechanobiology community. |
| format | Article |
| id | doaj-art-42bc9463504a4d7bbb59ecad22c06931 |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-42bc9463504a4d7bbb59ecad22c069312025-01-27T06:40:30ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-01-011310.3389/fbioe.2025.14945531494553Modular cone-and-plate device for mechanofluidic assays in Transwell insertsDaniel Chavarria0Kissamy A. Georges1Brian J. O’Grady2Khalid K. Hassan3Ethan S. Lippmann4Ethan S. Lippmann5Ethan S. Lippmann6Ethan S. Lippmann7Ethan S. Lippmann8Ethan S. Lippmann9Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, United StatesDepartment of Bioengineering, University of Massachusetts Dartmouth, Dartmouth, MA, United StatesDepartment of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, United StatesSchool for Science and Math at Vanderbilt, Vanderbilt University, Nashville, TN, United StatesDepartment of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, United StatesDepartment of Biomedical Engineering, Vanderbilt University, Nashville, TN, United StatesVanderbilt Brain Institute, Vanderbilt University, Nashville, TN, United StatesDepartment of Neurology, Vanderbilt University Medical Center, Nashville, TN, United StatesInterdisciplinary Materials Science Program, Vanderbilt University, Nashville, TN, United StatesVanderbilt Memory and Alzheimer’s Center, Vanderbilt University Medical Center, Nashville, TN, United StatesIn this work, we present a cost effective and open-source modular cone-and-plate (MoCAP) device that incorporates shear stress in the popular Transwell® insert system. This system acts as a lid that incorporates flow into 24-well Transwell® inserts while preserving the ability to conduct molecular profiling assays. Moreover, the MoCAP device can be rapidly reconfigured to test multiple shear stress profiles within a single device. To demonstrate the utility of the MoCAP, we conducted select assays on several different brain microvascular endothelial cell (BMEC) lines that comprise models of the blood-brain barrier (BBB), since shear stress can play an important role in BBB function. Our results characterize how shear stress modulates passive barrier function and GLUT1 expression across the different BMEC lines. Overall, we anticipate this low cost mechanofluidic device will be useful to the mechanobiology community.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1494553/fullblood-brain barriershear stressTranswell®endothelial cellmechanobiologyblood flow |
| spellingShingle | Daniel Chavarria Kissamy A. Georges Brian J. O’Grady Khalid K. Hassan Ethan S. Lippmann Ethan S. Lippmann Ethan S. Lippmann Ethan S. Lippmann Ethan S. Lippmann Ethan S. Lippmann Modular cone-and-plate device for mechanofluidic assays in Transwell inserts Frontiers in Bioengineering and Biotechnology blood-brain barrier shear stress Transwell® endothelial cell mechanobiology blood flow |
| title | Modular cone-and-plate device for mechanofluidic assays in Transwell inserts |
| title_full | Modular cone-and-plate device for mechanofluidic assays in Transwell inserts |
| title_fullStr | Modular cone-and-plate device for mechanofluidic assays in Transwell inserts |
| title_full_unstemmed | Modular cone-and-plate device for mechanofluidic assays in Transwell inserts |
| title_short | Modular cone-and-plate device for mechanofluidic assays in Transwell inserts |
| title_sort | modular cone and plate device for mechanofluidic assays in transwell inserts |
| topic | blood-brain barrier shear stress Transwell® endothelial cell mechanobiology blood flow |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1494553/full |
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