Comparative enrichment of complete ammonium oxidation bacteria in floccular sludge reactors: Sequencing batch reactor vs. continuous stirred tank reactor
This study attempted to compare the enrichment of complete ammonium oxidation (comammox) bacteria, which are affiliated with Nitrospira and not able to generate nitrous oxide (N2O, a potent greenhouse gas) through biological pathways, in two commonly-utilized configurations of floccular sludge react...
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Elsevier
2025-05-01
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| Series: | Water Research X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589914725000052 |
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| author | Ying Zhu Jiaying Hou Fangang Meng Meiying Xu Limin Lin Linyan Yang Xueming Chen |
| author_facet | Ying Zhu Jiaying Hou Fangang Meng Meiying Xu Limin Lin Linyan Yang Xueming Chen |
| author_sort | Ying Zhu |
| collection | DOAJ |
| description | This study attempted to compare the enrichment of complete ammonium oxidation (comammox) bacteria, which are affiliated with Nitrospira and not able to generate nitrous oxide (N2O, a potent greenhouse gas) through biological pathways, in two commonly-utilized configurations of floccular sludge reactors, i.e., sequencing batch reactor (SBR) and continuous stirred tank reactor (CSTR), under the ammonium condition of mainstream wastewater (i.e., 40.0 g-N/m3). The results in terms of nitrification performance and microbial analyses during 216-d operation showed that compared with SBR offering a fluctuating but generally higher in-situ ammonium concentration (i.e., 1.0–6.0 g-N/m3) which was favorable for the growth of ammonium-oxidizing bacteria (AOB, belonging to Nitrosomonas in this study), CSTR managed to lower the in-situ ammonium level to < 2.0 g-N/m3, thus creating a competitive advantage for comammox bacteria with a highly oligotrophic lifestyle. Such an argument was further supported by dedicated batch tests which revealed that Nitrospira-dominant sludge had a lower maximum ammonium oxidation rate and lower apparent ammonium and oxygen affinity constants than Nitrosomonas-dominant sludge (i.e., 33.5 ± 2.1 mg-N/h/g-MLVSS vs. 139.9 ± 26.7 mg-N/h/g-MLVSS, 1.1 ± 0.1 g-N/m3 vs. 17.6 ± 4.6 g-N/m3, and 0.017 ± 0.002 g-O2/m3 vs. 0.037 ± 0.013 g-O2/m3, respectively), proving the nature of comammox bacteria as a K-strategist. Overall, this study not only provided useful insights into the effective enrichment of comammox bacteria in floccular sludge but also further revealed the interactions between comammox bacteria and AOB, thereby contributing to the future development of comammox-inclusive biological nitrogen removal technologies for sustainable wastewater treatment. |
| format | Article |
| id | doaj-art-42a1e115ca1f4d83a77082ccd4d9aae5 |
| institution | Kabale University |
| issn | 2589-9147 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Water Research X |
| spelling | doaj-art-42a1e115ca1f4d83a77082ccd4d9aae52025-01-27T04:22:07ZengElsevierWater Research X2589-91472025-05-0127100305Comparative enrichment of complete ammonium oxidation bacteria in floccular sludge reactors: Sequencing batch reactor vs. continuous stirred tank reactorYing Zhu0Jiaying Hou1Fangang Meng2Meiying Xu3Limin Lin4Linyan Yang5Xueming Chen6College of Environment and Safety Engineering, Fuzhou University, Fuzhou 350116, PR ChinaCollege of Environment and Safety Engineering, Fuzhou University, Fuzhou 350116, PR ChinaGuangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology (Sun Yat-sen University), Guangzhou 510275, PR ChinaState Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR ChinaSchool of Engineering, Westlake University, Hangzhou, Zhejiang 310030, PR ChinaSchool of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, PR ChinaCollege of Environment and Safety Engineering, Fuzhou University, Fuzhou 350116, PR China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology (Sun Yat-sen University), Guangzhou 510275, PR China; State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China; Corresponding author.This study attempted to compare the enrichment of complete ammonium oxidation (comammox) bacteria, which are affiliated with Nitrospira and not able to generate nitrous oxide (N2O, a potent greenhouse gas) through biological pathways, in two commonly-utilized configurations of floccular sludge reactors, i.e., sequencing batch reactor (SBR) and continuous stirred tank reactor (CSTR), under the ammonium condition of mainstream wastewater (i.e., 40.0 g-N/m3). The results in terms of nitrification performance and microbial analyses during 216-d operation showed that compared with SBR offering a fluctuating but generally higher in-situ ammonium concentration (i.e., 1.0–6.0 g-N/m3) which was favorable for the growth of ammonium-oxidizing bacteria (AOB, belonging to Nitrosomonas in this study), CSTR managed to lower the in-situ ammonium level to < 2.0 g-N/m3, thus creating a competitive advantage for comammox bacteria with a highly oligotrophic lifestyle. Such an argument was further supported by dedicated batch tests which revealed that Nitrospira-dominant sludge had a lower maximum ammonium oxidation rate and lower apparent ammonium and oxygen affinity constants than Nitrosomonas-dominant sludge (i.e., 33.5 ± 2.1 mg-N/h/g-MLVSS vs. 139.9 ± 26.7 mg-N/h/g-MLVSS, 1.1 ± 0.1 g-N/m3 vs. 17.6 ± 4.6 g-N/m3, and 0.017 ± 0.002 g-O2/m3 vs. 0.037 ± 0.013 g-O2/m3, respectively), proving the nature of comammox bacteria as a K-strategist. Overall, this study not only provided useful insights into the effective enrichment of comammox bacteria in floccular sludge but also further revealed the interactions between comammox bacteria and AOB, thereby contributing to the future development of comammox-inclusive biological nitrogen removal technologies for sustainable wastewater treatment.http://www.sciencedirect.com/science/article/pii/S2589914725000052Ammonium-oxidizing bacteria (AOB)Complete ammonium oxidation (comammox) bacteriaContinuous stirred tank reactor (CSTR)r/K-strategistsSequencing batch reactor (SBR) |
| spellingShingle | Ying Zhu Jiaying Hou Fangang Meng Meiying Xu Limin Lin Linyan Yang Xueming Chen Comparative enrichment of complete ammonium oxidation bacteria in floccular sludge reactors: Sequencing batch reactor vs. continuous stirred tank reactor Water Research X Ammonium-oxidizing bacteria (AOB) Complete ammonium oxidation (comammox) bacteria Continuous stirred tank reactor (CSTR) r/K-strategists Sequencing batch reactor (SBR) |
| title | Comparative enrichment of complete ammonium oxidation bacteria in floccular sludge reactors: Sequencing batch reactor vs. continuous stirred tank reactor |
| title_full | Comparative enrichment of complete ammonium oxidation bacteria in floccular sludge reactors: Sequencing batch reactor vs. continuous stirred tank reactor |
| title_fullStr | Comparative enrichment of complete ammonium oxidation bacteria in floccular sludge reactors: Sequencing batch reactor vs. continuous stirred tank reactor |
| title_full_unstemmed | Comparative enrichment of complete ammonium oxidation bacteria in floccular sludge reactors: Sequencing batch reactor vs. continuous stirred tank reactor |
| title_short | Comparative enrichment of complete ammonium oxidation bacteria in floccular sludge reactors: Sequencing batch reactor vs. continuous stirred tank reactor |
| title_sort | comparative enrichment of complete ammonium oxidation bacteria in floccular sludge reactors sequencing batch reactor vs continuous stirred tank reactor |
| topic | Ammonium-oxidizing bacteria (AOB) Complete ammonium oxidation (comammox) bacteria Continuous stirred tank reactor (CSTR) r/K-strategists Sequencing batch reactor (SBR) |
| url | http://www.sciencedirect.com/science/article/pii/S2589914725000052 |
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