The Fate and Distribution of Autologous Bone Marrow Mesenchymal Stem Cells with Intra-Arterial Infusion in Osteonecrosis of the Femoral Head in Dogs
This study aimed to investigate if autologous bone marrow mesenchymal stem cells (MSCs) could treat osteonecrosis of the femoral head (ONFH) and what the fate and distribution of the cells are in dogs. Twelve Beagle dogs were randomly divided into two groups: MSCs group and SHAM op...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/8616143 |
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| author | Hongting Jin Taotao Xu Qiqing Chen Chengliang Wu Pinger Wang Qiang Mao Shanxing Zhang Jiayi Shen Peijian Tong |
| author_facet | Hongting Jin Taotao Xu Qiqing Chen Chengliang Wu Pinger Wang Qiang Mao Shanxing Zhang Jiayi Shen Peijian Tong |
| author_sort | Hongting Jin |
| collection | DOAJ |
| description | This study aimed to investigate if autologous bone marrow mesenchymal stem cells (MSCs)
could treat osteonecrosis of the femoral head (ONFH) and what the fate and distribution of the
cells are in dogs. Twelve Beagle dogs were randomly divided into two groups: MSCs group and
SHAM operated group. After three weeks, dogs in MSCs group and SHAM operated group were
intra-arterially injected with autologous MSCs and 0.9% normal saline, respectively. Eight
weeks after treatment, the necrotic volume of the femoral heads was significantly reduced in
MSCs group. Moreover, the trabecular bone volume was increased and the empty lacunae rate was
decreased in MSCs group. In addition, the BrdU-positive MSCs were unevenly distributed in femoral
heads and various vital organs. But no obvious abnormalities were observed. Furthermore, most of
BrdU-positive MSCs in necrotic region expressed osteocalcin in MSCs group and a few expressed
peroxisome proliferator-activated receptor-γ (PPAR-γ). Taken together, these data
indicated that intra-arterially infused MSCs could migrate into the necrotic field of femoral heads
and differentiate into osteoblasts, thus improving the necrosis of femoral heads. It suggests that
intra-arterial infusion of autologous MSCs might be a feasible and relatively safe method for the treatment of femoral head necrosis. |
| format | Article |
| id | doaj-art-429e2aa668ac48f1acbd30074cbf8bf5 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-429e2aa668ac48f1acbd30074cbf8bf52025-02-03T01:09:09ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/86161438616143The Fate and Distribution of Autologous Bone Marrow Mesenchymal Stem Cells with Intra-Arterial Infusion in Osteonecrosis of the Femoral Head in DogsHongting Jin0Taotao Xu1Qiqing Chen2Chengliang Wu3Pinger Wang4Qiang Mao5Shanxing Zhang6Jiayi Shen7Peijian Tong8Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, ChinaZhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, ChinaZhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, ChinaZhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, ChinaZhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, ChinaDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, ChinaZhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, ChinaZhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, ChinaDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, ChinaThis study aimed to investigate if autologous bone marrow mesenchymal stem cells (MSCs) could treat osteonecrosis of the femoral head (ONFH) and what the fate and distribution of the cells are in dogs. Twelve Beagle dogs were randomly divided into two groups: MSCs group and SHAM operated group. After three weeks, dogs in MSCs group and SHAM operated group were intra-arterially injected with autologous MSCs and 0.9% normal saline, respectively. Eight weeks after treatment, the necrotic volume of the femoral heads was significantly reduced in MSCs group. Moreover, the trabecular bone volume was increased and the empty lacunae rate was decreased in MSCs group. In addition, the BrdU-positive MSCs were unevenly distributed in femoral heads and various vital organs. But no obvious abnormalities were observed. Furthermore, most of BrdU-positive MSCs in necrotic region expressed osteocalcin in MSCs group and a few expressed peroxisome proliferator-activated receptor-γ (PPAR-γ). Taken together, these data indicated that intra-arterially infused MSCs could migrate into the necrotic field of femoral heads and differentiate into osteoblasts, thus improving the necrosis of femoral heads. It suggests that intra-arterial infusion of autologous MSCs might be a feasible and relatively safe method for the treatment of femoral head necrosis.http://dx.doi.org/10.1155/2016/8616143 |
| spellingShingle | Hongting Jin Taotao Xu Qiqing Chen Chengliang Wu Pinger Wang Qiang Mao Shanxing Zhang Jiayi Shen Peijian Tong The Fate and Distribution of Autologous Bone Marrow Mesenchymal Stem Cells with Intra-Arterial Infusion in Osteonecrosis of the Femoral Head in Dogs Stem Cells International |
| title | The Fate and Distribution of Autologous Bone Marrow Mesenchymal Stem Cells with Intra-Arterial Infusion in Osteonecrosis of the Femoral Head in Dogs |
| title_full | The Fate and Distribution of Autologous Bone Marrow Mesenchymal Stem Cells with Intra-Arterial Infusion in Osteonecrosis of the Femoral Head in Dogs |
| title_fullStr | The Fate and Distribution of Autologous Bone Marrow Mesenchymal Stem Cells with Intra-Arterial Infusion in Osteonecrosis of the Femoral Head in Dogs |
| title_full_unstemmed | The Fate and Distribution of Autologous Bone Marrow Mesenchymal Stem Cells with Intra-Arterial Infusion in Osteonecrosis of the Femoral Head in Dogs |
| title_short | The Fate and Distribution of Autologous Bone Marrow Mesenchymal Stem Cells with Intra-Arterial Infusion in Osteonecrosis of the Femoral Head in Dogs |
| title_sort | fate and distribution of autologous bone marrow mesenchymal stem cells with intra arterial infusion in osteonecrosis of the femoral head in dogs |
| url | http://dx.doi.org/10.1155/2016/8616143 |
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