The Fate and Distribution of Autologous Bone Marrow Mesenchymal Stem Cells with Intra-Arterial Infusion in Osteonecrosis of the Femoral Head in Dogs
This study aimed to investigate if autologous bone marrow mesenchymal stem cells (MSCs) could treat osteonecrosis of the femoral head (ONFH) and what the fate and distribution of the cells are in dogs. Twelve Beagle dogs were randomly divided into two groups: MSCs group and SHAM op...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/8616143 |
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| Summary: | This study aimed to investigate if autologous bone marrow mesenchymal stem cells (MSCs)
could treat osteonecrosis of the femoral head (ONFH) and what the fate and distribution of the
cells are in dogs. Twelve Beagle dogs were randomly divided into two groups: MSCs group and
SHAM operated group. After three weeks, dogs in MSCs group and SHAM operated group were
intra-arterially injected with autologous MSCs and 0.9% normal saline, respectively. Eight
weeks after treatment, the necrotic volume of the femoral heads was significantly reduced in
MSCs group. Moreover, the trabecular bone volume was increased and the empty lacunae rate was
decreased in MSCs group. In addition, the BrdU-positive MSCs were unevenly distributed in femoral
heads and various vital organs. But no obvious abnormalities were observed. Furthermore, most of
BrdU-positive MSCs in necrotic region expressed osteocalcin in MSCs group and a few expressed
peroxisome proliferator-activated receptor-γ (PPAR-γ). Taken together, these data
indicated that intra-arterially infused MSCs could migrate into the necrotic field of femoral heads
and differentiate into osteoblasts, thus improving the necrosis of femoral heads. It suggests that
intra-arterial infusion of autologous MSCs might be a feasible and relatively safe method for the treatment of femoral head necrosis. |
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| ISSN: | 1687-966X 1687-9678 |