IL-10 and socs3 Are Predictive Biomarkers of Dengue Hemorrhagic Fever
Background. Cytokines play important roles in the physiopathology of dengue infection; therefore, the suppressors of cytokine signaling (socs) that control the type and timing of cytokine functions could be involved in the origin of immune alterations in dengue. Objective. To explore the association...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/5197592 |
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| author | Lilian Karem Flores-Mendoza Tania Estrada-Jiménez Virginia Sedeño-Monge Margarita Moreno María del Consuelo Manjarrez Guadalupe González-Ochoa Lourdes Millán-Pérez Peña Julio Reyes-Leyva |
| author_facet | Lilian Karem Flores-Mendoza Tania Estrada-Jiménez Virginia Sedeño-Monge Margarita Moreno María del Consuelo Manjarrez Guadalupe González-Ochoa Lourdes Millán-Pérez Peña Julio Reyes-Leyva |
| author_sort | Lilian Karem Flores-Mendoza |
| collection | DOAJ |
| description | Background. Cytokines play important roles in the physiopathology of dengue infection; therefore, the suppressors of cytokine signaling (socs) that control the type and timing of cytokine functions could be involved in the origin of immune alterations in dengue. Objective. To explore the association of cytokine and socs levels with disease severity in dengue patients. Methods. Blood samples of 48 patients with confirmed dengue infection were analyzed. Amounts of interleukins IL-2, IL-4, IL-6, and IL-10, interferon- (IFN-) γ, and tumor necrosis factor- (TNF-) α were quantified by flow cytometry, and the relative expression of socs1 and socs3 mRNA was quantified by real-time RT-PCR. Results. Increased levels of IL-10 and socs3 and lower expression of socs1 were found in patients with dengue hemorrhagic fever (DHF) with respect to those with dengue fever (DF) (p<0.05). Negative correlations were found between socs1 and both IL-10 and socs3 (p<0.01). The cutoff values of socs3 (>199.8-fold), socs1 (<1.94-fold), and IL-10 (>134 pg/ml) have the highest sensitivity and specificity to discriminate between DF and DHF. Conclusion. Simultaneous changes in IL-10 and socs1/socs3 could be used as prognostic biomarkers of dengue severity. |
| format | Article |
| id | doaj-art-429a2f7cbf424769ab4af3faee7688e3 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-429a2f7cbf424769ab4af3faee7688e32025-02-03T01:09:08ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/51975925197592IL-10 and socs3 Are Predictive Biomarkers of Dengue Hemorrhagic FeverLilian Karem Flores-Mendoza0Tania Estrada-Jiménez1Virginia Sedeño-Monge2Margarita Moreno3María del Consuelo Manjarrez4Guadalupe González-Ochoa5Lourdes Millán-Pérez Peña6Julio Reyes-Leyva7Centro de Investigación Biomédica de Oriente, HGZ5, Instituto Mexicano del Seguro Social, Km 4.5 Carretera Atlixco-Metepec, 74360 Metepec, PUE, MexicoCentro de Investigación Biomédica de Oriente, HGZ5, Instituto Mexicano del Seguro Social, Km 4.5 Carretera Atlixco-Metepec, 74360 Metepec, PUE, MexicoDepartamento de Ciencias de la Salud, Universidad Popular Autónoma del Estado de Puebla, 21 Sur 1103, 72410 Puebla, PUE, MexicoHospital General de Zona No. 5, Instituto Mexicano del Seguro Social, Km 4.5 Carretera Atlixco-Metepec, 74360 Metepec, PUE, MexicoHospital General de Zona No. 5, Instituto Mexicano del Seguro Social, Km 4.5 Carretera Atlixco-Metepec, 74360 Metepec, PUE, MexicoDepartamento de Ciencias Químico Biológicas y Agropecuarias, División de Ciencias e Ingeniería, Universidad de Sonora, 85880 Navojoa, SON, MexicoLaboratorio de Bioquímica y Biología Molecular, Centro de Química, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edif. 103 F, CU-BUAP, San Manuel, 72570 Puebla, PUE, MexicoCentro de Investigación Biomédica de Oriente, HGZ5, Instituto Mexicano del Seguro Social, Km 4.5 Carretera Atlixco-Metepec, 74360 Metepec, PUE, MexicoBackground. Cytokines play important roles in the physiopathology of dengue infection; therefore, the suppressors of cytokine signaling (socs) that control the type and timing of cytokine functions could be involved in the origin of immune alterations in dengue. Objective. To explore the association of cytokine and socs levels with disease severity in dengue patients. Methods. Blood samples of 48 patients with confirmed dengue infection were analyzed. Amounts of interleukins IL-2, IL-4, IL-6, and IL-10, interferon- (IFN-) γ, and tumor necrosis factor- (TNF-) α were quantified by flow cytometry, and the relative expression of socs1 and socs3 mRNA was quantified by real-time RT-PCR. Results. Increased levels of IL-10 and socs3 and lower expression of socs1 were found in patients with dengue hemorrhagic fever (DHF) with respect to those with dengue fever (DF) (p<0.05). Negative correlations were found between socs1 and both IL-10 and socs3 (p<0.01). The cutoff values of socs3 (>199.8-fold), socs1 (<1.94-fold), and IL-10 (>134 pg/ml) have the highest sensitivity and specificity to discriminate between DF and DHF. Conclusion. Simultaneous changes in IL-10 and socs1/socs3 could be used as prognostic biomarkers of dengue severity.http://dx.doi.org/10.1155/2017/5197592 |
| spellingShingle | Lilian Karem Flores-Mendoza Tania Estrada-Jiménez Virginia Sedeño-Monge Margarita Moreno María del Consuelo Manjarrez Guadalupe González-Ochoa Lourdes Millán-Pérez Peña Julio Reyes-Leyva IL-10 and socs3 Are Predictive Biomarkers of Dengue Hemorrhagic Fever Mediators of Inflammation |
| title | IL-10 and socs3 Are Predictive Biomarkers of Dengue Hemorrhagic Fever |
| title_full | IL-10 and socs3 Are Predictive Biomarkers of Dengue Hemorrhagic Fever |
| title_fullStr | IL-10 and socs3 Are Predictive Biomarkers of Dengue Hemorrhagic Fever |
| title_full_unstemmed | IL-10 and socs3 Are Predictive Biomarkers of Dengue Hemorrhagic Fever |
| title_short | IL-10 and socs3 Are Predictive Biomarkers of Dengue Hemorrhagic Fever |
| title_sort | il 10 and socs3 are predictive biomarkers of dengue hemorrhagic fever |
| url | http://dx.doi.org/10.1155/2017/5197592 |
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