Caffeic Acid Phenethyl Ester Regulates PPAR’s Levels in Stem Cells-Derived Adipocytes
Hypertrophic obesity inhibits activation of peroxisome proliferators-activated receptor gamma (PPARγ), considered the key mediator of the fully differentiated and insulin sensitive adipocyte phenotype. We examined the effects of Caffeic Acid Phenethyl Ester (Cape), isolated from propolis, a honeybee...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2016/7359521 |
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| author | Luca Vanella Daniele Tibullo Justyna Godos Francesca Romana Pluchinotta Claudia Di Giacomo Valeria Sorrenti Rosaria Acquaviva Alessandra Russo Giovanni Li Volti Ignazio Barbagallo |
| author_facet | Luca Vanella Daniele Tibullo Justyna Godos Francesca Romana Pluchinotta Claudia Di Giacomo Valeria Sorrenti Rosaria Acquaviva Alessandra Russo Giovanni Li Volti Ignazio Barbagallo |
| author_sort | Luca Vanella |
| collection | DOAJ |
| description | Hypertrophic obesity inhibits activation of peroxisome proliferators-activated receptor gamma (PPARγ), considered the key mediator of the fully differentiated and insulin sensitive adipocyte phenotype. We examined the effects of Caffeic Acid Phenethyl Ester (Cape), isolated from propolis, a honeybee hive product, on Adipose Stem Cells (ASCs) differentiation to the adipocyte lineage. Finally we tested the effects of Cape on insulin-resistant adipocytes. Quantification of Oil Red O-stained cells showed that lipid droplets decreased following Cape treatment as well as radical oxygen species formation. Additionally, exposure of ASC to high glucose levels decreased adiponectin and increased proinflammatory cytokines mRNA levels, which were reversed by Cape-mediated increase of insulin sensitivity. Cape treatment resulted in decreased triglycerides synthesis and increased beta-oxidation. Exposure of ASCs to Lipopolysaccharide (LPS) induced a reduction of PPARγ, an increase of IL-6 levels associated with a well-known stimulation of lipolysis; Cape partially attenuated the LPS-mediated effects. These observations reveal the main role of PPARγ in the adipocyte function and during ASC differentiation. As there is now substantial interest in functional food and nutraceutical products, the observed therapeutic value of Cape in insulin-resistance related diseases should be taken into consideration. |
| format | Article |
| id | doaj-art-428b908d03254ba5bfc1cfbb84742ab2 |
| institution | Kabale University |
| issn | 1687-4757 1687-4765 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | PPAR Research |
| spelling | doaj-art-428b908d03254ba5bfc1cfbb84742ab22025-02-03T01:24:52ZengWileyPPAR Research1687-47571687-47652016-01-01201610.1155/2016/73595217359521Caffeic Acid Phenethyl Ester Regulates PPAR’s Levels in Stem Cells-Derived AdipocytesLuca Vanella0Daniele Tibullo1Justyna Godos2Francesca Romana Pluchinotta3Claudia Di Giacomo4Valeria Sorrenti5Rosaria Acquaviva6Alessandra Russo7Giovanni Li Volti8Ignazio Barbagallo9Department of Drug Science, Biochemistry Section, University of Catania, Viale Andrea Doria 6, 95125 Catania, ItalyDepartment of Surgery and Medical Specialties, Section of Hematology, University of Catania, Via Salvatore Citelli 6, 95124 Catania, ItalyDepartment of Drug Science, Biochemistry Section, University of Catania, Viale Andrea Doria 6, 95125 Catania, ItalyIRCCS “S. Donato” Hospital, San Donato Milanese, Piazza Edmondo Malan, 20097 Milan, ItalyDepartment of Drug Science, Biochemistry Section, University of Catania, Viale Andrea Doria 6, 95125 Catania, ItalyDepartment of Drug Science, Biochemistry Section, University of Catania, Viale Andrea Doria 6, 95125 Catania, ItalyDepartment of Drug Science, Biochemistry Section, University of Catania, Viale Andrea Doria 6, 95125 Catania, ItalyDepartment of Drug Science, Biochemistry Section, University of Catania, Viale Andrea Doria 6, 95125 Catania, ItalyDepartment of Biomedicine and Biotechnologies, University of Catania, Viale Andrea Doria 6, 95125 Catania, ItalyDepartment of Drug Science, Biochemistry Section, University of Catania, Viale Andrea Doria 6, 95125 Catania, ItalyHypertrophic obesity inhibits activation of peroxisome proliferators-activated receptor gamma (PPARγ), considered the key mediator of the fully differentiated and insulin sensitive adipocyte phenotype. We examined the effects of Caffeic Acid Phenethyl Ester (Cape), isolated from propolis, a honeybee hive product, on Adipose Stem Cells (ASCs) differentiation to the adipocyte lineage. Finally we tested the effects of Cape on insulin-resistant adipocytes. Quantification of Oil Red O-stained cells showed that lipid droplets decreased following Cape treatment as well as radical oxygen species formation. Additionally, exposure of ASC to high glucose levels decreased adiponectin and increased proinflammatory cytokines mRNA levels, which were reversed by Cape-mediated increase of insulin sensitivity. Cape treatment resulted in decreased triglycerides synthesis and increased beta-oxidation. Exposure of ASCs to Lipopolysaccharide (LPS) induced a reduction of PPARγ, an increase of IL-6 levels associated with a well-known stimulation of lipolysis; Cape partially attenuated the LPS-mediated effects. These observations reveal the main role of PPARγ in the adipocyte function and during ASC differentiation. As there is now substantial interest in functional food and nutraceutical products, the observed therapeutic value of Cape in insulin-resistance related diseases should be taken into consideration.http://dx.doi.org/10.1155/2016/7359521 |
| spellingShingle | Luca Vanella Daniele Tibullo Justyna Godos Francesca Romana Pluchinotta Claudia Di Giacomo Valeria Sorrenti Rosaria Acquaviva Alessandra Russo Giovanni Li Volti Ignazio Barbagallo Caffeic Acid Phenethyl Ester Regulates PPAR’s Levels in Stem Cells-Derived Adipocytes PPAR Research |
| title | Caffeic Acid Phenethyl Ester Regulates PPAR’s Levels in Stem Cells-Derived Adipocytes |
| title_full | Caffeic Acid Phenethyl Ester Regulates PPAR’s Levels in Stem Cells-Derived Adipocytes |
| title_fullStr | Caffeic Acid Phenethyl Ester Regulates PPAR’s Levels in Stem Cells-Derived Adipocytes |
| title_full_unstemmed | Caffeic Acid Phenethyl Ester Regulates PPAR’s Levels in Stem Cells-Derived Adipocytes |
| title_short | Caffeic Acid Phenethyl Ester Regulates PPAR’s Levels in Stem Cells-Derived Adipocytes |
| title_sort | caffeic acid phenethyl ester regulates ppar s levels in stem cells derived adipocytes |
| url | http://dx.doi.org/10.1155/2016/7359521 |
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