Non-Invasive Point-of-Care Detection of Methamphetamine and Cocaine via Aptamer-Based Lateral Flow Test
Drug abuse is a major public problem in the workplace, traffic, and forensic issues, which requires a standardized test device to monitor on-site drug use. For field testing, the most important requirements are portability, sensitivity, non-invasiveness, and quick results. Motivated by this problem,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-01-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/1/31 |
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| author | Bilge Erkocyigit Ezgi Man Ece Efecan Ozge Ozufuklar Deniz Devecioglu Basak Bagci Ebru Aldemir Hakan Coskunol Serap Evran Emine Guler Celik |
| author_facet | Bilge Erkocyigit Ezgi Man Ece Efecan Ozge Ozufuklar Deniz Devecioglu Basak Bagci Ebru Aldemir Hakan Coskunol Serap Evran Emine Guler Celik |
| author_sort | Bilge Erkocyigit |
| collection | DOAJ |
| description | Drug abuse is a major public problem in the workplace, traffic, and forensic issues, which requires a standardized test device to monitor on-site drug use. For field testing, the most important requirements are portability, sensitivity, non-invasiveness, and quick results. Motivated by this problem, a point of care (POC) test based on lateral flow assay (LFA) was developed for the detection of cocaine (COC) and methamphetamine (MET) in saliva which has been selected as the matrix for this study due to its rapid and non-invasive collection process. In the design strategy of an LFA test, the use of gold nanoparticles (AuNPs) with strong optical properties has been combined with the advantages of selecting aptamers under in vitro conditions, making it a highly specific and stable recognition probe for the detection of small molecules in saliva. The developed aptamer-based LFA in a competitive format, was able to detect COC and MET in synthetic saliva at concentrations as low as 5.0 ng/mL. After analytical performance studies, the test system also detected COC and MET in real patient samples, which was verified by chromatographic methods. |
| format | Article |
| id | doaj-art-4276c2b687f84b669f0767ec104a20b9 |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-4276c2b687f84b669f0767ec104a20b92025-01-24T13:25:29ZengMDPI AGBiosensors2079-63742025-01-011513110.3390/bios15010031Non-Invasive Point-of-Care Detection of Methamphetamine and Cocaine via Aptamer-Based Lateral Flow TestBilge Erkocyigit0Ezgi Man1Ece Efecan2Ozge Ozufuklar3Deniz Devecioglu4Basak Bagci5Ebru Aldemir6Hakan Coskunol7Serap Evran8Emine Guler Celik9Department of Biotechnology, Institute of Natural Sciences, Ege University, 35040 Izmir, TürkiyeDepartment of Biochemistry, Faculty of Science, Ege University, 35040 Izmir, TürkiyeDepartment of Biotechnology, Institute of Natural Sciences, Ege University, 35040 Izmir, TürkiyeDepartment of Biotechnology, Institute of Natural Sciences, Ege University, 35040 Izmir, TürkiyeDepartment of Psychiatry, Ataturk Educational and Research Hospital, Katip Celebi University, 35360 Izmir, TürkiyeDepartment of Psychiatry, Ataturk Educational and Research Hospital, Katip Celebi University, 35360 Izmir, TürkiyeDepartment of Psychiatry, Faculty of Medicine, Izmir Tinaztepe University, 35400 Izmir, TürkiyeDepartment of Psychiatry, Faculty of Medicine, Ege University, 35040 Izmir, TürkiyeDepartment of Biochemistry, Faculty of Science, Ege University, 35040 Izmir, TürkiyeDepartment of Bioengineering, Faculty of Engineering, Ege University, 35040 Izmir, TürkiyeDrug abuse is a major public problem in the workplace, traffic, and forensic issues, which requires a standardized test device to monitor on-site drug use. For field testing, the most important requirements are portability, sensitivity, non-invasiveness, and quick results. Motivated by this problem, a point of care (POC) test based on lateral flow assay (LFA) was developed for the detection of cocaine (COC) and methamphetamine (MET) in saliva which has been selected as the matrix for this study due to its rapid and non-invasive collection process. In the design strategy of an LFA test, the use of gold nanoparticles (AuNPs) with strong optical properties has been combined with the advantages of selecting aptamers under in vitro conditions, making it a highly specific and stable recognition probe for the detection of small molecules in saliva. The developed aptamer-based LFA in a competitive format, was able to detect COC and MET in synthetic saliva at concentrations as low as 5.0 ng/mL. After analytical performance studies, the test system also detected COC and MET in real patient samples, which was verified by chromatographic methods.https://www.mdpi.com/2079-6374/15/1/31lateral flow assayaptamerpoint-of-caresaliva drug testabuse drug testing |
| spellingShingle | Bilge Erkocyigit Ezgi Man Ece Efecan Ozge Ozufuklar Deniz Devecioglu Basak Bagci Ebru Aldemir Hakan Coskunol Serap Evran Emine Guler Celik Non-Invasive Point-of-Care Detection of Methamphetamine and Cocaine via Aptamer-Based Lateral Flow Test Biosensors lateral flow assay aptamer point-of-care saliva drug test abuse drug testing |
| title | Non-Invasive Point-of-Care Detection of Methamphetamine and Cocaine via Aptamer-Based Lateral Flow Test |
| title_full | Non-Invasive Point-of-Care Detection of Methamphetamine and Cocaine via Aptamer-Based Lateral Flow Test |
| title_fullStr | Non-Invasive Point-of-Care Detection of Methamphetamine and Cocaine via Aptamer-Based Lateral Flow Test |
| title_full_unstemmed | Non-Invasive Point-of-Care Detection of Methamphetamine and Cocaine via Aptamer-Based Lateral Flow Test |
| title_short | Non-Invasive Point-of-Care Detection of Methamphetamine and Cocaine via Aptamer-Based Lateral Flow Test |
| title_sort | non invasive point of care detection of methamphetamine and cocaine via aptamer based lateral flow test |
| topic | lateral flow assay aptamer point-of-care saliva drug test abuse drug testing |
| url | https://www.mdpi.com/2079-6374/15/1/31 |
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