The anti-cancerous mechanism of licochalcone A on human hepatoma cell HepG2 based on the miRNA omics
To explore the function of licochalcone A as an anticancer phytochemical on HepG2 cells and investigate its potential mechanisms, we analyzed the microRNAs (miRNAs) expression profile of HepG2 cells in response to licochalcone A (70 μmol/L) in vitro. 102 dysregulated miRNAs were detected, and SP1 wa...
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Tsinghua University Press
2023-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002580 |
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| author | Jun Wang Xiuxiu Zhang Zhijing Ni Elnur Elam Kiran Thakur Kexin Li Chuyan Wang Jianguo Zhang Zhaojun Wei |
| author_facet | Jun Wang Xiuxiu Zhang Zhijing Ni Elnur Elam Kiran Thakur Kexin Li Chuyan Wang Jianguo Zhang Zhaojun Wei |
| author_sort | Jun Wang |
| collection | DOAJ |
| description | To explore the function of licochalcone A as an anticancer phytochemical on HepG2 cells and investigate its potential mechanisms, we analyzed the microRNAs (miRNAs) expression profile of HepG2 cells in response to licochalcone A (70 μmol/L) in vitro. 102 dysregulated miRNAs were detected, and SP1 was expected as the transcription factor that regulates the functions of most screened miRNAs. A sum of 431 targets, the overlap of predicted mRNAs from TargetScan, miRDB, and miRtarbase were detected as the targets for these dysregulated miRNAs. FoxO signaling pathway was the hub pathway for the targets. A protein-protein interaction network was structured on the STRING platform to discover the hub genes. Among them, PIK3R1, CDC42, ESR1, SMAD4, SUMO1, KRAS, AGO1, etc. were screened out. Afterwards, the miRNA-target networks were established to screen key dysregulated miRNAs. Two key miRNAs (hsa-miR-133b and hsa-miR-145-5p) were filtered. Finally, the miRNA-target-transcription factor networks were constructed for these key miRNAs. The networks for these key miRNAs included three and two transcription factors, respectively. These identified miRNAs, transcription factors, targets, and regulatory networks may offer hints to understand the molecular mechanism of licochalcone A as a natural anticarcinogen. |
| format | Article |
| id | doaj-art-426f1afc102e4966af59c21b31e7f5b9 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-426f1afc102e4966af59c21b31e7f5b92025-02-03T04:05:21ZengTsinghua University PressFood Science and Human Wellness2213-45302023-07-0112411361148The anti-cancerous mechanism of licochalcone A on human hepatoma cell HepG2 based on the miRNA omicsJun Wang0Xiuxiu Zhang1Zhijing Ni2Elnur Elam3Kiran Thakur4Kexin Li5Chuyan Wang6Jianguo Zhang7Zhaojun Wei8School of Biological Food and Environment, Hefei University, Hefei 230601, China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Biological Science and Engineering, Ningxia Key Laboratory for the Development and Application of Microbial Resources in Extreme Environments, North Minzu University, Yinchuan 750021, ChinaSchool of Biological Science and Engineering, Ningxia Key Laboratory for the Development and Application of Microbial Resources in Extreme Environments, North Minzu University, Yinchuan 750021, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China; School of Biological Science and Engineering, Ningxia Key Laboratory for the Development and Application of Microbial Resources in Extreme Environments, North Minzu University, Yinchuan 750021, ChinaSchool of Biological Food and Environment, Hefei University, Hefei 230601, ChinaSchool of Biological Food and Environment, Hefei University, Hefei 230601, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China; School of Biological Science and Engineering, Ningxia Key Laboratory for the Development and Application of Microbial Resources in Extreme Environments, North Minzu University, Yinchuan 750021, China; Corresponding authors at: School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China; School of Biological Science and Engineering, Ningxia Key Laboratory for the Development and Application of Microbial Resources in Extreme Environments, North Minzu University, Yinchuan 750021, China; Corresponding authors at: School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.To explore the function of licochalcone A as an anticancer phytochemical on HepG2 cells and investigate its potential mechanisms, we analyzed the microRNAs (miRNAs) expression profile of HepG2 cells in response to licochalcone A (70 μmol/L) in vitro. 102 dysregulated miRNAs were detected, and SP1 was expected as the transcription factor that regulates the functions of most screened miRNAs. A sum of 431 targets, the overlap of predicted mRNAs from TargetScan, miRDB, and miRtarbase were detected as the targets for these dysregulated miRNAs. FoxO signaling pathway was the hub pathway for the targets. A protein-protein interaction network was structured on the STRING platform to discover the hub genes. Among them, PIK3R1, CDC42, ESR1, SMAD4, SUMO1, KRAS, AGO1, etc. were screened out. Afterwards, the miRNA-target networks were established to screen key dysregulated miRNAs. Two key miRNAs (hsa-miR-133b and hsa-miR-145-5p) were filtered. Finally, the miRNA-target-transcription factor networks were constructed for these key miRNAs. The networks for these key miRNAs included three and two transcription factors, respectively. These identified miRNAs, transcription factors, targets, and regulatory networks may offer hints to understand the molecular mechanism of licochalcone A as a natural anticarcinogen.http://www.sciencedirect.com/science/article/pii/S2213453022002580Licochalcone AHepG2 cellsDysregulated miRNAsTranscription factorsTargetsRegulatory networks |
| spellingShingle | Jun Wang Xiuxiu Zhang Zhijing Ni Elnur Elam Kiran Thakur Kexin Li Chuyan Wang Jianguo Zhang Zhaojun Wei The anti-cancerous mechanism of licochalcone A on human hepatoma cell HepG2 based on the miRNA omics Food Science and Human Wellness Licochalcone A HepG2 cells Dysregulated miRNAs Transcription factors Targets Regulatory networks |
| title | The anti-cancerous mechanism of licochalcone A on human hepatoma cell HepG2 based on the miRNA omics |
| title_full | The anti-cancerous mechanism of licochalcone A on human hepatoma cell HepG2 based on the miRNA omics |
| title_fullStr | The anti-cancerous mechanism of licochalcone A on human hepatoma cell HepG2 based on the miRNA omics |
| title_full_unstemmed | The anti-cancerous mechanism of licochalcone A on human hepatoma cell HepG2 based on the miRNA omics |
| title_short | The anti-cancerous mechanism of licochalcone A on human hepatoma cell HepG2 based on the miRNA omics |
| title_sort | anti cancerous mechanism of licochalcone a on human hepatoma cell hepg2 based on the mirna omics |
| topic | Licochalcone A HepG2 cells Dysregulated miRNAs Transcription factors Targets Regulatory networks |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002580 |
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